公开(公告)号：US08501422B2

公开(公告)日：2013-08-06

申请号：US13120189

申请日：2009-09-23

Applicant: Csanky Eszter ,  Ralph Ruhl ,  Laszlo Takacs ,  William Hempel

Inventor： Csanky Eszter ,  Ralph Ruhl ,  Laszlo Takacs ,  William Hempel

IPC: G01N31/00 ,  G01N33/53

CPC classification number: G01N33/6893 ,  G01N2800/122

Abstract: The present invention relates to the treatment of pulmonary diseases. More specifically, the invention relates to new methods of detecting and treating chronic obstructive pulmonary disease (COPD). In particular, the invention relates to a method of measuring one or more lipid metabolites in human body fluids as an indicator/biomarker of the progress of chronic obstructive pulmonary disease. The present invention also relates to a method of detecting and/or monitoring chronic obstructive pulmonary disease in a subject, the method comprising measuring the level of at least one lipid metabolite in a sample from the subject, wherein said level is indicative of COPD. The present invention also relates to a method of assessing the efficacy of a COPD treatment in a subject, the method comprising a step of measuring the level of at least one lipid metabolite in a sample from the subject, wherein said level is indicative of COPD severity or status.

Abstract translation: 本发明涉及肺部疾病的治疗。 更具体地，本发明涉及检测和治疗慢性阻塞性肺疾病（COPD）的新方法。 特别地，本发明涉及测量人体液体中的一种或多种脂质代谢物作为慢性阻塞性肺疾病进展的指标/生物标志物的方法。 本发明还涉及检测和/或监测受试者中慢性阻塞性肺病的方法，所述方法包括测量来自受试者的样品中至少一种脂质代谢物的水平，其中所述水平指示COPD。 本发明还涉及一种评估受试者中COPD治疗功效的方法，所述方法包括测量来自受试者的样品中至少一种脂质代谢物的水平的步骤，其中所述水平指示COPD严重性 或状态。

公开(公告)号：US06277259B1

公开(公告)日：2001-08-21

申请号：US09298800

申请日：1999-04-23

Applicant: Andras Guttman ,  Laszlo Takacs

Inventor： Andras Guttman ,  Laszlo Takacs

IPC: G01N2726

CPC classification number: G01N27/44795 ,  G01N27/44773

Abstract: A proteome analyzer includes a separation cassette having a first dimension separation compartment for separation of protein samples by isoelectric focusing and a second dimension separation compartment for separation of protein samples by SDS-polymer network electrophoresis. The first dimension compartment is a reservoir in which a porous material having capillary channels is disposed. The protein samples are disposed in the capillary channels and, in the presence of a pH gradient, are focused spatially by isolectric focusing upon application of an electric field. The second dimension compartment consists of two glass or plastic plates separated by a separation medium. The separation medium is an ultra-thin layer of a low concentration linear polymer supported by an inert matrix. The spatially focused protein samples are contacted with the separation medium in the presence of an electric field to initiate second dimension separation. The migrating SDS-protein complexes are fluorescently labeled by a dye in the separation medium during second dimension separation, and are detected by an illumination and detection system as they pass through a detection area. The detected SDS-protein complexes are imaged and displayed by computer analysis as two-dimensional maps representing the proteins in the sample.

Abstract translation: 蛋白质组分析仪包括具有用于通过等电聚焦分离蛋白质样品的第一维分离室和用于通过SDS-聚合物网络电泳分离蛋白质样品的第二维分离室的分离盒。 第一尺寸隔室是其中布置有具有毛细通道的多孔材料的储存器。 将蛋白质样品置于毛细通道中，并且在pH梯度的存在下，通过施加电场的等电聚焦在空间上聚焦。 第二维室由两个玻璃或塑料板组成，分隔介质分开。 分离介质是由惰性基质负载的低浓度线性聚合物的超薄层。 空间聚焦的蛋白质样品在电场的存在下与分离介质接触以启动第二维分离。 迁移的SDS-蛋白复合物在第二维分离期间由分离介质中的染料荧光标记，并且在通过检测区域时由照射和检测系统检测。 检测到的SDS-蛋白复合物通过计算机分析成像和显示，作为代表样品中的蛋白质的二维图。

公开(公告)号：US20140364326A1

公开(公告)日：2014-12-11

申请号：US13981725

申请日：2012-01-30

Applicant: Mariana Guergova-Kuras ,  Yann Kieffer ,  Carole Malderez-Bloes ,  Alexandra Kremeurt ,  Laszlo Takacs

Inventor： Mariana Guergova-Kuras ,  Yann Kieffer ,  Carole Malderez-Bloes ,  Alexandra Kremeurt ,  Laszlo Takacs

IPC: G01N33/574

CPC classification number: G01N33/57423 ,  G01N2333/4742

Abstract: The present invention relates to compositions and methods for detecting, managing or monitoring cancer. The invention also relates to antibodies specific for cancer markers, compositions and chips containing the same, as well as their uses for cancer detection, managing, monitoring, imaging or treatment, as well as for drug development. The invention is particularly suited for detecting, managing or monitoring lung cancer in human subjects.

Abstract translation: 本发明涉及用于检测，管理或监测癌症的组合物和方法。 本发明还涉及针对癌症标志物，含有其的组合物和芯片以及其用于癌症检测，管理，监测，成像或治疗以及药物开发的特异性抗体。 本发明特别适用于检测，管理或监测人类受试者的肺癌。

公开(公告)号：US20070293437A1

公开(公告)日：2007-12-20

申请号：US11665539

申请日：2005-10-21

Applicant: Andras Guttman ,  Laszlo Takacs

Inventor： Andras Guttman ,  Laszlo Takacs

IPC: A61K38/00 ,  G01N33/00 ,  G01N33/53

CPC classification number: G01N33/6803 ,  G01N2550/00

Abstract: The present invention relates to an efficient mAb panel-based expression profiling technology platform suitable for global and accurate measurement of proteins, peptides and metabolites in complex mixtures. The platform is comprised of new and well established technologies that are coupled in a unique fashion to provide a novel platform technology for (i) the discovery of differentially displayed elements of complex protein, peptide and metabolite mixtures and (ii) the development of robust mAb based assays that detect the differentially expressed elements.

Abstract translation: 本发明涉及一种有效的基于mAb面板的表达谱分析技术平台，适用于全面准确地测量复杂混合物中的蛋白质，肽和代谢物。 该平台由新的和成熟的技术组成，以独特的方式相结合，为（i）发现差异显示的复杂蛋白质，肽和代谢物混合物的元素提供了一种新的平台技术，以及（ii）开发强大的mAb 检测差异表达的元件。

公开(公告)号：US20070172887A1

公开(公告)日：2007-07-26

申请号：US10588392

申请日：2005-02-09

Applicant: Laszlo Takacs ,  Andras Guttrman ,  William Hancock ,  Barry Karger ,  Manuel Duval ,  Patrick Berna

Inventor： Laszlo Takacs ,  Andras Guttrman ,  William Hancock ,  Barry Karger ,  Manuel Duval ,  Patrick Berna

IPC: G01N33/53 ,  C12M3/00

CPC classification number: G01N33/6854 ,  B01L3/5027 ,  C07K16/18 ,  G01N33/54386 ,  G01N33/577 ,  G01N33/6845 ,  G01N2500/00

Abstract: A method or platform for monoclonal antibody based biomarker discovery is disclosed. The method according to the invention provides for the integration of analyte collection, hybridoma screening and nanovolume integrated mass spectrometry (NVIMS) to achieve a robust screening system that is capable, for example, of cutting 4-6 years off of the classical biomarker discovery and development process. The invention provides a platform for the rapid, high-throughput production, isolation and characterization of, e.g., disease specific biomarkers together with highly specific monoclonal antibodies. The method of the invention has a variety of applications such as, but not limited to, drug testing, biohazard applications, ecological applications, physiological applications and/or pathology screening applications. The method of the invention is also capable of being performed or used as or with a high-throughput screening process or system of the invention.

Abstract translation: 公开了用于基于单克隆抗体的生物标志物发现的方法或平台。 根据本发明的方法提供了分析物收集，杂交瘤筛选和纳瓦级综合质谱（NVIMS）的整合，以实现强大的筛选系统，其能够例如从经典生物标志物发现开始4-6年， 开发过程。 本发明提供了用于快速，高通量生产，分离和表征例如疾病特异性生物标志物以及高度特异性单克隆抗体的平台。 本发明的方法具有多种应用，例如但不限于药物测试，生物危害应用，生态应用，生理应用和/或病理学筛选应用。 本发明的方法还能够作为或与本发明的高通量筛选方法或系统一起使用或使用。

公开(公告)号：US20060012881A1

公开(公告)日：2006-01-19

申请号：US11186959

申请日：2005-07-22

Applicant: Gerald Mearini ,  Laszlo Takacs

Inventor： Gerald Mearini ,  Laszlo Takacs

IPC: G02B1/10

CPC classification number: C23C14/547 ,  C23C14/5833 ,  C23C14/5873

Abstract: An optical filter using alternating layers of materials with “low” and “high” indices of refraction and deposited with atomic layer control has been developed. The multilayered thin film filter uses, but is not limited to, alternating amorphous layers of atomically controlled Si (n=3.56) as the high index material and diamond-like carbon (DLC, n=2.0) as the low index material. The Si layers are grown with a self-limiting pulsed molecular beam deposition process which results in layer-by-layer growth and thickness control to within one atomic layer. The DLC layers are produced using an ion-based process and made atomically smooth using a modified Chemical Reactive-Ion Surface Planarization (CRISP) process. Intrinsic stress is monitored using an in-situ cantilever-based intrinsic stress optical monitor and adjusted during filter fabrication by deposition parameter modification. The resulting filter has sufficient individual layer thickness control and surface roughness to enable ˜12.5 GHz filters for next generation multiplexers and demultiplexers with more than 1000 channels in the wavelength range 1.31-1.62 μm.

公开(公告)号：US06893500B2

公开(公告)日：2005-05-17

申请号：US09865152

申请日：2001-05-24

Applicant: Gerald T. Mearini ,  Laszlo Takacs

Inventor： Gerald T. Mearini ,  Laszlo Takacs

IPC: C23C14/54 ,  C23C16/56 ,  C30B25/16

CPC classification number: C23C16/56 ,  C23C14/547

Abstract: A method of constructing optical filters using alternating layers of materials with “low” and “high” indices of refraction and deposited with atomic layer control. The multilayered thin film filter uses, but is not limited to, alternating layers of single crystal, polycrystalline or amorphous materials grown with self-limiting epitaxial deposition processes well known to the semiconductor industry. The deposition process, such as atomic layer epitaxy (ALE), pulsed chemical beam epitaxy (PCB E), molecular layer epitaxy (MLE) or laser molecular beam epitaxy (laser MBE) can result in epitaxial layer by layer growth and thickness control to within one atomic layer. The alternating layers are made atomically smooth using a Chemical Reactive-Ion Surface Planarization (CRISP) process. Intrinsic stress is monitored using an in-situ cantilever based intrinsic stress optical monitor and adjusted during filter fabrication by deposition parameter modification. The resulting filter has sufficient individual layer thickness control and surface roughness to enable ˜12.5 GHz filters for next generation multiplexers and demultiplexers with more than 1000 channels in the wavelength range 1.31-1.62 μm.

Abstract translation: 使用具有“低”和“高”折射率的材料的交替层来构造光学滤光器并用原子层控制沉积的方法。 多层薄膜滤光器使用但不限于用半导体工业公知的自限制外延沉积工艺生长的单晶，多晶或非晶材料的交替层。 原子层外延（ALE），脉冲化学束外延（PCB E），分子层外延（MLE）或激光分子束外延（激光MBE）等沉积过程可以使外延逐层生长和厚度控制到内部 一个原子层。 使用化学反应离子表面平面化（CRISP）方法将交替层制成原子平滑的。 使用基于原位悬臂的固有应力光学监测器监测内在应力，并通过沉积参数修改在过滤器制造期间进行调整。 所得到的滤波器具有足够的单独层厚度控制和表面粗糙度，以便在波长1.31-1.62μm的范围内为具有多于1000个通道的下一代多路复用器和解复用器提供〜12.5GHz滤波器。

公开(公告)号：US20050037371A1

公开(公告)日：2005-02-17

申请号：US10723522

申请日：2003-11-26

Applicant: Jean-Jacques Codani ,  Guillaume Dufresne ,  Manuel Duval ,  Eric Glemet ,  Hendrik Heus ,  Laszlo Takacs

Inventor： Jean-Jacques Codani ,  Guillaume Dufresne ,  Manuel Duval ,  Eric Glemet ,  Hendrik Heus ,  Laszlo Takacs

IPC: C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/22 ,  G06N3/00 ,  G06F19/00

CPC classification number: G16B30/00

Abstract: Methods and systems for comparing a first sequence and a second sequence, including associating errors with alignments of the first sequence and the second sequence, comparing the alignment errors to identify the alignment having the smallest error, and, based on the alignment having the smallest error, computing: a first percent identity relative to the first sequence, and a second percent identity relative to the second sequence.

Abstract translation: 用于比较第一序列和第二序列的方法和系统，包括将错误与第一序列和第二序列的比对相关联，比较对准误差以识别具有最小误差的对准，并且基于具有最小误差的对准 ，计算：相对于第一序列的第一百分比身份，以及相对于第二序列的第二百分比身份。

公开(公告)号：US06767475B2

公开(公告)日：2004-07-27

申请号：US09864583

申请日：2001-05-24

Applicant: Gerald T. Mearini ,  Laszlo Takacs

Inventor： Gerald T. Mearini ,  Laszlo Takacs

IPC: B44C122

CPC classification number: C23C14/5833 ,  C23C14/547 ,  C23C14/5873 ,  C23C16/56 ,  H01J37/3056 ,  H01J2237/3174 ,  H01L21/31051

Abstract: An oxygen ion process, Chemical Reactive-Ion Surface Planarization (CRISP), has been developed which enables planarization of thin film surfaces at the atomic level. Narrow/broad band filters produced with vacuum deposited multilayered thin films are designed to selectively reflect/transmit light at specific wavelengths. The optical performance is limited by the ability to control the individual layer thickness, the “roughness” of the individual layer surfaces and the stoichiometry of the layers. The process described herein will enable reduction of surface roughness at the interfaces of multilayered thin films to produce atomically smooth surfaces. The application of this process will result in the production of notch filters of less than 0.3 nm full width at half maximum (FWHM) centered at the desired wavelength. This will enable optical filters designed for telecommunication components such as next generation dense wavelength division multiplexer (DWDM) systems with significant performance improvement beyond the state-of-the-art.

Abstract translation: 已经开发了氧离子过程，化学反应离子表面平面化（CRISP），其能够在原子水平上平坦化薄膜表面。 用真空沉积的多层薄膜制成的窄带/宽带滤光片被设计成选择性地反射/透射特定波长的光。 光学性能受到控制单层厚度，单层表面的“粗糙度”和各层的化学计量的能力的限制。 本文所述的方法将能够减少多层薄膜的界面处的表面粗糙度，以产生原子光滑的表面。 该方法的应用将导致生产以所需波长为中心的半峰全宽（FWHM）小于0.3nm的陷波滤波器。 这将使得为诸如下一代密集波分多路复用器（DWDM）系统等电信部件设计的光学滤波器具有超越现有技术的显着性能改进。

公开(公告)号：US20120021532A1

公开(公告)日：2012-01-26

申请号：US13120189

申请日：2009-09-23

Applicant: Csanky Eszter ,  Ralph Ruhl ,  Laszlo Takacs ,  William Hempel

Inventor： Csanky Eszter ,  Ralph Ruhl ,  Laszlo Takacs ,  William Hempel

IPC: G01N33/53 ,  H01J49/26

CPC classification number: G01N33/6893 ,  G01N2800/122

Abstract: The present invention relates to the treatment of pulmonary diseases. More specifically, the invention relates to new methods of detecting and treating chronic obstructive pulmonary disease (COPD). In particular, the invention relates to a method of measuring one or more lipid metabolites in human body fluids as an indicator/biomarker of the progress of chronic obstructive pulmonary disease. The present invention also relates to a method of detecting and/or monitoring chronic obstructive pulmonary disease in a subject, the method comprising measuring the level of at least one lipid metabolite in a sample from the subject, wherein said level is indicative of COPD. The present invention also relates to a method of assessing the efficacy of a COPD treatment in a subject, the method comprising a step of measuring the level of at least one lipid metabolite in a sample from the subject, wherein said level is indicative of COPD severity or status.

Abstract translation: 本发明涉及肺部疾病的治疗。 更具体地，本发明涉及检测和治疗慢性阻塞性肺疾病（COPD）的新方法。 特别地，本发明涉及测量人体液体中的一种或多种脂质代谢物作为慢性阻塞性肺疾病进展的指标/生物标志物的方法。 本发明还涉及一种检测和/或监测受试者中慢性阻塞性肺病的方法，所述方法包括测量来自受试者的样品中至少一种脂质代谢物的水平，其中所述水平指示COPD。 本发明还涉及一种评价受试者中COPD治疗功效的方法，所述方法包括测量来自受试者的样品中至少一种脂质代谢物的水平的步骤，其中所述水平指示COPD严重性 或状态。