公开(公告)号：US5134065A

公开(公告)日：1992-07-28

申请号：US864070

申请日：1986-05-16

Applicant: Michael A. Sanzo ,  Arthur J. Wittwer ,  Jayne C. Marasa ,  Joseph Feder

Inventor： Michael A. Sanzo ,  Arthur J. Wittwer ,  Jayne C. Marasa ,  Joseph Feder

IPC: C07K14/81

CPC classification number: C07K14/8132

Abstract: A novel and unique plasminogen activator inhibitor fragment is obtained from human umbilical vein endothelial cells which has the following characteristics:A. it is derived from a native t-PA inhibitor that binds to and inhibits the activity of t-PA,B. it is dissociated from a complex formed between said native t-PA inhibitor and t-PA, said complex existing in two distinct interconvertible conformations with molecular weight of about 88 KDa and 105 KDa, respectively, and being partially reversible in the presence of fibrin,C. it has a molecular weight of about 40 KDa when dissociated from the complex, andD. it has a novel partial N-terminal amino acid sequence when dissociated from the complex.

Abstract translation: 从人脐静脉内皮细胞获得一种新颖独特的纤溶酶原激活物抑制剂片段，其具有以下特征：A.它衍生自结合并抑制t-PA，B的活性的天然t-PA抑制剂。它是 从所述天然t-PA抑制剂和t-PA之间形成的复合物解离，所述复合物分别存在于分子量约88KDa和105KDa的两种不同的可互变构象中，并且在纤维蛋白C存在下部分可逆。 当从络合物中解离时，其具有约40KDa的分子量，以及D.当从络合物解离时，其具有新的部分N-末端氨基酸序列。

公开(公告)号：US5132214A

公开(公告)日：1992-07-21

申请号：US849933

申请日：1986-04-09

Applicant: Joseph Feder ,  Nicholaos K. Harakas ,  Jon P. Schaumann ,  Daniel T. Connolly ,  Arthur J. Wittwer

Inventor： Joseph Feder ,  Nicholaos K. Harakas ,  Jon P. Schaumann ,  Daniel T. Connolly ,  Arthur J. Wittwer

IPC: C12N9/64 ,  A61K38/00 ,  C07K1/14 ,  C08B37/00 ,  C12N5/00 ,  C12N9/72 ,  C12R1/91

CPC classification number: C12N9/6459 ,  C08B37/00 ,  C12Y304/21069 ,  A61K38/00

Abstract: Plasminogen activators (PA) are obtained from cultured normal human colon cells which are adaptable to large scale production. A purified tissue PA (t-PA) is obtained from CCD-18Co normal human colon fibroblast cells which shows chemical differences from Bowes melanoma t-PA.

Abstract translation: 纤维蛋白溶酶原激活物（PA）是从适合大规模生产的培养的正常人结肠细胞获得的。 从CT-18Co正常人结肠成纤维细胞获得纯化的组织PA（t-PA），其显示与Bowes黑素瘤t-PA的化学差异。

公开(公告)号：US5036003A

公开(公告)日：1991-07-30

申请号：US240780

申请日：1988-09-02

Applicant: Jitka V. Olander ,  Daniel T. Connolly ,  Steven P. Adams ,  Joseph Feder

Inventor： Jitka V. Olander ,  Daniel T. Connolly ,  Steven P. Adams ,  Joseph Feder

IPC: C07K14/52 ,  C12P21/08

CPC classification number: C07K14/52

Abstract: A method of producing antibodies against vascular permeability factor (VPF) is disclosed which comprises immunizing a host animal with a peptide having an amino acid sequence as follows: ##STR1## or a fragment of said peptide containing an antigenic determinant of VPF.

Abstract translation: 公开了一种产生针对血管通透性因子（VPF）的抗体的方法，其包括用具有如下氨基酸序列的肽免疫宿主动物：或者含有抗原决定簇的所述肽的片段 的VPF。

公开(公告)号：US4851517A

公开(公告)日：1989-07-25

申请号：US159992

申请日：1988-02-24

Applicant: Joseph Feder ,  William R. Tolbert ,  Thomas W. Rademacher ,  Raj B. Parekh ,  Raymond A. Dwek

Inventor： Joseph Feder ,  William R. Tolbert ,  Thomas W. Rademacher ,  Raj B. Parekh ,  Raymond A. Dwek

IPC: A61K38/00 ,  C08B37/00 ,  C12N9/72

CPC classification number: C12N9/6459 ,  C08B37/00 ,  C12Y304/21069 ,  A61K38/00

Abstract: Glycosylated plasminogen activator (t-PA) having a glycosylation pattern significantly different than exhibited by t-PA from Bowes melanoma cells is obtained from cultured normal human colon fibroblast cells (CCD-18Co).

Abstract translation: 从培养的正常人结肠成纤维细胞（CCD-18Co）获得具有与Bowes黑素瘤细胞的t-PA显示不同的糖基化模式的糖基化纤溶酶原激活剂（t-PA）。

公开(公告)号：US4833085A

公开(公告)日：1989-05-23

申请号：US896362

申请日：1986-08-13

Applicant: Jon P. Schaumann ,  Jitka V. Olander ,  Nicholaos K. Harakas ,  Joseph Feder

Inventor： Jon P. Schaumann ,  Jitka V. Olander ,  Nicholaos K. Harakas ,  Joseph Feder

IPC: G01N33/577 ,  A61K39/395 ,  C07K1/22 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/30 ,  C07K16/40 ,  C12N5/00 ,  C12N15/00 ,  C12N15/02 ,  C12P21/00 ,  C12P21/08 ,  C12R1/91 ,  G01N33/53 ,  G01N33/573

CPC classification number: C07K16/3046 ,  C07K16/40

Abstract: A monoclonal antibody is disclosed which is specific for human colon fibroblast-derived tissue plasminogen activator (t-PA), and which is useful for immunoaffinity chromatography purification of t-PA and determination of t-PA in a biological sample.

Abstract translation: 公开了对人结肠成纤维细胞衍生的组织纤溶酶原激活物（t-PA）特异的单克隆抗体，其可用于t-PA的免疫亲和层析纯化和生物样品中t-PA的测定。

公开(公告)号：US4268629A

公开(公告)日：1981-05-19

申请号：US126811

申请日：1980-03-03

Applicant: William R. Tolbert ,  Mau-Jung Kuo ,  Joseph Feder

Inventor： William R. Tolbert ,  Mau-Jung Kuo ,  Joseph Feder

IPC: C12P1/00

CPC classification number: C12P1/00 ,  Y10S435/948

Abstract: Process for the production of human angiogenic factor in vitro comprising growing the human diploid cell line IMR-90 on support surface in nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate angiogenic factor and recovering the resulting angiogenic factor from the cells or cell product.

Abstract translation: 用于在体外生产人血管生成因子的方法，包括在约35-38℃下在营养培养基中的载体表面上培养人二倍体细胞系IMR-90足够的时间以制备血管生成因子并回收所得的血管生成因子 从细胞或细胞产物。

公开(公告)号：US4210718A

公开(公告)日：1980-07-01

申请号：US974403

申请日：1978-12-29

Applicant: William R. Tolbert ,  Joseph Feder ,  Mau-Jung Kuo

Inventor： William R. Tolbert ,  Joseph Feder ,  Mau-Jung Kuo

IPC: C07K14/47 ,  C07K14/515 ,  C12P1/00

CPC classification number: C07K14/515 ,  C07K14/4748 ,  Y10S435/948

Abstract: Process for the production of human TAF in vitro comprising growing the human melanoma cell line A-375 in agitated, liquid suspension of nutrient culture medium at about 35.degree.-38.degree. C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.

Abstract translation: 用于在体外生产人TAF的方法包括在约35-38℃的营养培养基的搅拌的液体悬浮液中培养人黑素瘤细胞系A-375足够的时间以制备TAF并从所得的TAF中回收所得的TAF 细胞或细胞产物。

公开(公告)号：US4187149A

公开(公告)日：1980-02-05

申请号：US881458

申请日：1978-02-27

Applicant: William R. Tolbert ,  Joseph Feder

Inventor： William R. Tolbert ,  Joseph Feder

IPC: A61L29/04 ,  C12M1/26 ,  C12B1/00

CPC classification number: C12M33/04 ,  A61L29/041 ,  C12M33/14

Abstract: A cell culture sampling device is provided which comprises a double septum holder having an axial bore therethrough, upper and lower septa retaining members, said upper retaining member having lip means to retain immobilized an external septum and said lower retaining member having shoulder means to retain immobilized an internal septum, said lip and shoulder means being spaced apart to thereby define a fixed chamber between said immobilized septa.

Abstract translation: 提供了一种细胞培养取样装置，其包括具有穿过其中的轴向孔的双隔膜保持器，上隔膜和下隔片保持构件，所述上保持构件具有用于保持固定的外隔膜的唇部装置，并且所述下保持构件具有肩部装置以保持固定 内部隔膜，所述唇部和肩部装置间隔开，从而在所述固定的间隔之间限定固定室。

公开(公告)号：US4184916A

公开(公告)日：1980-01-22

申请号：US918898

申请日：1978-06-26

Applicant: William R. Tolbert ,  Joseph Feder ,  Richard C. Kimes

Inventor： William R. Tolbert ,  Joseph Feder ,  Richard C. Kimes

IPC: B01D29/11 ,  C12M3/02 ,  C12K9/00

CPC classification number: C12M23/08 ,  B01D29/11 ,  C12M27/02 ,  C12M29/04

Abstract: A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly having a hollow shaft means journaled for rotation in a stationary sleeve sub-assembly and a filter unit suspended downwardly therefrom, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means and adapted for coaxial rotation with said shaft means.

Abstract translation: 提供了一种细胞培养系统和装置，用于哺乳动物细胞的悬浮培养，其中可以添加新鲜培养基，并在连续或半连续的基础上过滤和抽出废培养基，而不会细胞破坏。 该装置包括中空的烧瓶组件，其具有中空轴装置，该空心轴装置用于在固定的套筒子组件中旋转并且从其向下悬挂的过滤器单元，所述过滤器单元通过所述中空部分的内部与烧瓶的顶部开口流体连通 轴装置并且适于与所述轴装置同轴旋转。

公开(公告)号：US4087327A

公开(公告)日：1978-05-02

申请号：US676100

申请日：1976-04-12

Applicant: Joseph Feder ,  Katharine Ku ,  Mau-Jung Kuo

Inventor： Joseph Feder ,  Katharine Ku ,  Mau-Jung Kuo

IPC: C12M3/04 ,  C12M3/06 ,  C12N5/02 ,  C12N5/06 ,  C12K9/00 ,  C12B1/10

CPC classification number: C12M23/04 ,  C12M29/16

Abstract: A cell culture reactor system is provided for the growth of cells in vitro which employs elongate hollow or solid fibers arranged in a shallow layer configuration as a matrix for cell attachment in which the flow path of the culture media is directed substantially uniformly through the fiber layer and substantially transverse to the plane of the elongate axes of the fibers.

Abstract translation: 提供细胞培养反应器系统用于体外细胞的生长，其采用以浅层构型布置的细长的中空或固体纤维作为细胞附着的基质，其中培养基的流动路径基本均匀地通过纤维层 并且基本上横向于纤维的细长轴的平面。