公开(公告)号：US08635029B2

公开(公告)日：2014-01-21

申请号：US12238216

申请日：2008-09-25

Applicant: Claes Gustafsson ,  Sridhar Govindarajan ,  Jeremy Minshull

Inventor： Claes Gustafsson ,  Sridhar Govindarajan ,  Jeremy Minshull

IPC: G06F7/00

CPC classification number: G06F19/18

Abstract: Methods, computer systems, and computer program products for biopolymer engineering. A variant set for a biopolymer of interest is constructed by identifying, using a plurality of rules, a plurality of positions in the biopolymer of interest and, for each respective position in the plurality of positions, substitutions for the respective position. The plurality of positions and the substitutions for each respective position in the plurality of positions collectively define a biopolymer sequence space. A variant set comprising a plurality of variants of the biopolymer of interest is selected. A property of all or a portion of the variants in the variant set is measured. A sequence-activity relationship is modeled between (i) one or more substitutions at one or more positions of the biopolymer of interest represented by the variant set and (ii) the property measured for all or the portion of the variants in the variant set. The variant set is redefined to comprise variants that include substitutions in the plurality of positions that are selected based on a function of the sequence-activity relationship.

Abstract translation: 方法，计算机系统和生物聚合物工程的计算机程序产品。 通过使用多个规则来识别感兴趣的生物聚合物中的多个位置，并且对于多个位置中的每个相应位置，通过识别相应位置的替代来构建用于感兴趣的生物聚合物的变体。 多个位置中的各个位置的多个位置和替换共同限定生物聚合物序列空间。 选择包含感兴趣的生物聚合物的多个变体的变体组。 测量变体集中全部或部分变体的属性。 在（i）由变体组表示的感兴趣的生物聚合物的一个或多个位置处的一个或多个取代和（ii）针对变体组中的全部或部分变体测量的特性的序列活性关系进行建模。 变体集被重新定义为包括在基于序列活动关系的函数选择的多个位置中的替换的变体。

公开(公告)号：US08058001B2

公开(公告)日：2011-11-15

申请号：US12557829

申请日：2009-09-11

Applicant: Andreas Crameri ,  Willem P. C. Stemmer ,  Jeremy Minshull ,  Steven H. Bass ,  Mark Welch ,  John E. Ness ,  Claes Gustafsson ,  Phillip A. Patten

Inventor： Andreas Crameri ,  Willem P. C. Stemmer ,  Jeremy Minshull ,  Steven H. Bass ,  Mark Welch ,  John E. Ness ,  Claes Gustafsson ,  Phillip A. Patten

IPC: C12Q1/68 ,  C12N15/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12P21/06

Abstract: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

Abstract translation: 提供重组核酸的方法，包括同源核酸。 还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。

公开(公告)号：US07999152B2

公开(公告)日：2011-08-16

申请号：US12534470

申请日：2009-08-03

Applicant: Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Cristina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Nicholas B. Duck ,  Rebecca Gorton ,  Billy Fred McCutchen ,  Roger Kemble

Inventor： Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Cristina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Nicholas B. Duck ,  Rebecca Gorton ,  Billy Fred McCutchen ,  Roger Kemble

IPC: A01H5/00 ,  C12N15/82 ,  C12N5/00 ,  C12N5/04 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C12N9/10 ,  C12P21/06

CPC classification number: C12N15/8275 ,  C12N9/1029 ,  C12N9/1092 ,  C12N15/8209 ,  C12Y205/01019

Abstract: Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

公开(公告)号：US20110083231A1

公开(公告)日：2011-04-07

申请号：US12952562

申请日：2010-11-23

Applicant: Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Cristina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Rebecca Gorton ,  Nicholas B. Duck

Inventor： Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Cristina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Rebecca Gorton ,  Nicholas B. Duck

IPC: C12N15/82

CPC classification number: C12N9/1029 ,  C12N15/8275

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract translation: 本文提供了新的蛋白质，包括能够催化草甘膦和其他结构相关蛋白质的乙酰化的蛋白质。 还提供了能够编码这些蛋白质的新型多核苷酸，包含这些新蛋白质和/或多核苷酸中的一种或多种的组合物，包含这些新化合物的重组细胞和转基因植物，涉及新化合物的多样化方法，以及使用该化合物的方法。 本文提供的一些新颖的方法和化合物可以用于产生对草甘膦有抗性的生物，如植物。

公开(公告)号：US07795030B2

公开(公告)日：2010-09-14

申请号：US12011767

申请日：2008-01-28

Applicant: Jeremy Minshull ,  Willem P. C. Stemmer

Inventor： Jeremy Minshull ,  Willem P. C. Stemmer

IPC: C12N15/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12N15/1027 ,  C12N15/1058

Abstract: The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.

Abstract translation: 本发明一般涉及新代谢途径的演变以及通过本文中称为递归序列重组的方法增强生物处理。 递归序列重组需要执行重组和筛选或选择的“进化”个体基因，整个质粒或病毒，多基因簇或甚至全基因组的迭代循环。 这些技术不需要传统的代谢工程方法所需的广泛的分析和计算。

公开(公告)号：US20090298714A1

公开(公告)日：2009-12-03

申请号：US12534405

申请日：2009-08-03

Applicant: Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Christina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Rebecca Gorton ,  Nicholas B. Duck ,  Billy Fred McCutchen ,  Roger Kemble

Inventor： Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Christina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Rebecca Gorton ,  Nicholas B. Duck ,  Billy Fred McCutchen ,  Roger Kemble

IPC: C40B40/08 ,  C07H21/00 ,  C07H21/04 ,  C12N15/63

CPC classification number: C12N15/8275 ,  C12N9/1029 ,  C12N9/1092 ,  C12N15/8209 ,  C12Y205/01019

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

公开(公告)号：US20090298123A1

公开(公告)日：2009-12-03

申请号：US12184901

申请日：2008-08-01

Applicant: Lorraine J. Giver ,  Jeremy Minshull ,  Kurt Vogel

Inventor： Lorraine J. Giver ,  Jeremy Minshull ,  Kurt Vogel

IPC: C12P21/02 ,  C12N9/16 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

CPC classification number: C12N9/20 ,  A61K38/00 ,  C12N15/1034

Abstract: New lipase enzymes (both nucleic acids and polypeptides) are provided. Compositions which include these polypeptides, proteins, nucleic acids, recombinant cells, as well as methods involving the enzymes, antibodies to the enzymes, and methods of using the enzymes are also provided.

Abstract translation: 提供新的脂肪酶（核酸和多肽）。 还提供了包括这些多肽，蛋白质，核酸，重组细胞以及涉及酶的方法，酶的抗体以及使用酶的方法的组合物。

公开(公告)号：US07620502B2

公开(公告)日：2009-11-17

申请号：US11339090

申请日：2006-01-24

Applicant: Sergey A. Selifonov ,  Willem P. C. Stemmer ,  Claes Gustafsson ,  Matthew Tobin ,  Stephen del Cardayre ,  Phillip A. Patten ,  Jeremy Minshull ,  Lorraine J. Giver

Inventor： Sergey A. Selifonov ,  Willem P. C. Stemmer ,  Claes Gustafsson ,  Matthew Tobin ,  Stephen del Cardayre ,  Phillip A. Patten ,  Jeremy Minshull ,  Lorraine J. Giver

IPC: C01N33/48 ,  C12Q1/00 ,  C12Q1/48 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: G06F19/16 ,  B01J2219/007 ,  C40B50/02 ,  G06F19/14 ,  G06F19/18 ,  G06F19/22 ,  G06F19/24

Abstract: In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators are used to identify sets of oligonucleotides for in vitro recombination.

Abstract translation: 在计算机核酸重组方法中，利用遗传算子的相关综合系统用于鉴定用于体外重组的寡核苷酸组。

公开(公告)号：US07507569B2

公开(公告)日：2009-03-24

申请号：US10555484

申请日：2004-05-06

Applicant: Stefan Minning ,  Jurgen Carsten Franz Knotzel ,  Niels Henrik Sorensen ,  Jon E. Ness ,  Mark D. Welch ,  Lorraine J. Giver ,  Joel Cherry ,  Torben Vedel Borchert ,  Jeremy Minshull

Inventor： Stefan Minning ,  Jurgen Carsten Franz Knotzel ,  Niels Henrik Sorensen ,  Jon E. Ness ,  Mark D. Welch ,  Lorraine J. Giver ,  Joel Cherry ,  Torben Vedel Borchert ,  Jeremy Minshull

IPC: C12N9/54 ,  C12N15/57 ,  C12N15/74 ,  C11D3/386

CPC classification number: C11D3/38636 ,  C11D3/38681 ,  C12N9/52 ,  Y02P20/582

Abstract: Novel subtilases having an improved wash performance on egg stains are disclosed. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.

Abstract translation: 公开了具有改进的蛋渍洗涤性能的新型脱水酶。 当用于例如蛋白质时，这些脱水酶可用于在蛋渍上表现出优异或改善的洗涤性能。 清洁剂或洗涤剂组合物，例如衣物洗涤剂组合物和洗碟组合物，包括自动洗碗组合物。

公开(公告)号：US07462481B2

公开(公告)日：2008-12-09

申请号：US10427692

申请日：2003-04-30

Applicant: Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Christina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Rebecca Gorton ,  Nicholas B. Duck ,  Billy Fred McCutchen ,  Roger Kemble

Inventor： Linda A. Castle ,  Dan Siehl ,  Lorraine Giver ,  Jeremy Minshull ,  Christina Ivy ,  Yong Hong Chen ,  Phillip A. Patten ,  Rebecca Gorton ,  Nicholas B. Duck ,  Billy Fred McCutchen ,  Roger Kemble

IPC: C12N9/12 ,  C12N9/10 ,  C12N15/00 ,  C12P21/06 ,  C07H21/04 ,  A01H1/00 ,  A01H5/00

CPC classification number: C12N15/8275 ,  C12N9/1029 ,  C12N9/1092 ,  C12N15/8209 ,  C12Y205/01019

Abstract: Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Abstract translation: 本文提供了新的蛋白质，包括能够催化草甘膦和其他结构相关蛋白质的乙酰化的蛋白质。 还提供了能够编码这些蛋白质的新型多核苷酸，包含这些新蛋白质和/或多核苷酸中的一种或多种的组合物，包含这些新化合物的重组细胞和转基因植物，涉及新化合物的多样化方法，以及使用该化合物的方法。 本文提供的一些新颖的方法和化合物可以用于产生对草甘膦有抗性的生物，如植物。