公开(公告)号：US6146857A

公开(公告)日：2000-11-14

申请号：US43830

申请日：1998-06-15

Applicant: Gilles Pauly ,  Marc Pauly ,  Jean-Marc Engasser ,  Mohamed Ghoul

Inventor： Gilles Pauly ,  Marc Pauly ,  Jean-Marc Engasser ,  Mohamed Ghoul

IPC: A61K8/00 ,  A61K8/11 ,  A61K8/72 ,  A61K8/73 ,  A61K8/99 ,  A61Q17/04 ,  A61Q19/00 ,  C08B37/18 ,  C12N1/00 ,  C12N1/06 ,  C12N1/14 ,  C12P19/04 ,  C12R1/86 ,  A61K7/035 ,  C12P1/02

CPC classification number: A61K8/73 ,  A61Q19/00 ,  C12N1/063 ,  C12P19/04 ,  A61Q17/04 ,  Y10S435/911 ,  Y10S435/921 ,  Y10S435/94

Abstract: A process is provided for the production of glycogens or an extract rich in glycogens from yeast cells, and a cosmetic composition containing them. A given quantity of yeast cells, from a specific culture or recovered as residues of a fermentation process, is subjected to an operation of enrichment in intracellular glycogens by culturing in two phases in the presence of a carbon source. The metabolism of the yeast cells is then stopped. The membranes of the yeast cells are then at least partially disintegrated to free intracellular substances, and the freed intracellular substances are subjected to at least one precipation to precipitate glycogens. A cosmetic composition comprising the glycogens is formulated in admixture with a dermatologically acceptable excipient.

Abstract translation: PCT No.PCT / FR96 / 02008 Sec。 371日期1998年6月15日第 102（e）1998年6月15日PCT PCT 1996年12月13日PCT公布。 公开号WO97 / 21828 日期1996年6月19日提供了用于从酵母细胞中制备糖原或富含糖原的提取物的方法和含有它们的化妆品组合物的方法。 给定量的来自特定培养物的酵母细胞或作为发酵过程的残留物回收的酵母细胞通过在碳源的存在下在两相中培养来进行细胞内糖原的富集操作。 然后停止酵母细胞的代谢。 然后将酵母细胞的膜至少部分地分解成游离细胞内物质，并且释放的细胞内物质经历至少一个沉淀以沉淀糖原。 含有糖原的化妆品组合物与皮肤病学可接受的赋形剂混合配制。

公开(公告)号：US20060115880A1

公开(公告)日：2006-06-01

申请号：US10537627

申请日：2003-11-22

Applicant: Mohamed Ghoul ,  Jean-Marc Engasser ,  Philippe Moussou ,  Gilles Pauly ,  Melika Ardhaoui ,  Aude Falcimaigne

Inventor： Mohamed Ghoul ,  Jean-Marc Engasser ,  Philippe Moussou ,  Gilles Pauly ,  Melika Ardhaoui ,  Aude Falcimaigne

IPC: C12P17/06

CPC classification number: C12P7/62 ,  C12P17/06

Abstract: Disclosed is a method for enzymatic synthesis of esters of flavonoid and flavonoid derivatives, according to which a reaction medium containing an organic solvent, a glycosylated flavonoid or aglycon flavonoid, an acyl group donor, and an enzymatic catalyst is produced, b) additional quantities of flavonoid and/or acyl donor are optionally added during the reaction, and c) the obtained esters are separated from the enzymatic particles and the solvent. The inventive method is characterized by maintaining the concentration of water and/or alcohol which are formed during the reaction to below 150 mM. Advantageously, the molar ratio of flavonoid to acyl donor in the reaction medium is maintained in the range from 0.01 to 20.00 during the reaction.

Abstract translation: 公开了一种用于酶促合成类黄酮和类黄酮衍生物的酯的方法，根据该方法，产生含有有机溶剂，糖基化黄酮或苷元类黄酮，酰基供体和酶催化剂的反应介质，b） 在反应期间任选地加入类黄酮和/或酰基供体，和c）将所得酯与酶微粒和溶剂分离。 本发明的方法的特征在于将反应期间形成的水和/或醇的浓度保持在150mM以下。 有利地，在反应期间，反应介质中类黄酮与酰基供体的摩尔比保持在0.01至20.00的范围内。

公开(公告)号：US06355455B1

公开(公告)日：2002-03-12

申请号：US09462427

申请日：2000-01-10

Applicant: Gilles Pauly ,  Jean-Marc Engasser ,  Mohammed Ghoul

Inventor： Gilles Pauly ,  Jean-Marc Engasser ,  Mohammed Ghoul

IPC: C12P762

CPC classification number: C12P7/64 ,  C12P19/02 ,  C12P19/44

Abstract: A method for the enzymatic synthesis of sucrose ester, comprises introducing, in an adapted reactor and so as to form a reaction medium, predetermined amounts of an organic solvent, a sugar or a sugar derivative, a compound donor of acyl groups and an enzymatic catalyst, the amount of at least one constituent of the reaction mixture being deficient, in controlled addition during the reaction of additional amounts of the deficient constituent(s), and finally purifying the resulting sucrose esters at least by separating the fine enzymatic particles from the solvent.

Abstract translation: 用于酶合成蔗糖酯的方法包括在适应的反应器中引入预定量的有机溶剂，糖或糖衍生物，酰基的化合物供体和酶催化剂 ，反应混合物中至少一种组分的量是不足的，在反应期间加入额外量的缺陷组分，最后纯化得到的蔗糖酯，至少通过从溶剂中分离出细的酶促颗粒 。