公开(公告)号：US07833783B2

公开(公告)日：2010-11-16

申请号：US11485240

申请日：2006-07-12

申请人： Deborah Haines ,  Gordon Allan ,  John Ellis ,  Brian Meehan ,  Edward Clark ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly ,  Li Wang ,  Lorne A. Babiuk ,  Andrew A. Potter ,  Philip Willson

发明人： Deborah Haines ,  Gordon Allan ,  John Ellis ,  Brian Meehan ,  Edward Clark ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly ,  Li Wang ,  Lorne A. Babiuk ,  Andrew A. Potter ,  Philip Willson

IPC分类号： C12N15/00 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/53 ,  A61K2039/552 ,  A61K2039/70 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10043 ,  C12Q1/701

摘要： The cloning of a novel PCVII viral genome is described as is expression of proteins derived from the PCVII genome. These proteins can be used in vaccine compositions for the prevention and treatment of PCVII infections, as well as in diagnostic methods for determining the presence of PCVII infections in a vertebrate subject. Polynucleotides derived from the viral genome can be used as diagnostic primers and probes.

摘要翻译： 新型PCVII病毒基因组的克隆描述为源自PCVII基因组的蛋白质的表达。 这些蛋白质可用于预防和治疗PCVII感染的疫苗组合物中，以及用于确定脊椎动物受试者中PCVII感染存在的诊断方法。 衍生自病毒基因组的多核苷酸可用作诊断引物和探针。

公开(公告)号：US07803613B2

公开(公告)日：2010-09-28

申请号：US11485525

申请日：2006-07-12

申请人： Deborah Haines ,  Gordon Allan ,  John Ellis ,  Brian Meehan ,  Edward Clark ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly ,  Li Wang ,  Lorne A. Babiuk ,  Andrew A. Potter ,  Philip Willson

发明人： Deborah Haines ,  Gordon Allan ,  John Ellis ,  Brian Meehan ,  Edward Clark ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly ,  Li Wang ,  Lorne A. Babiuk ,  Andrew A. Potter ,  Philip Willson

IPC分类号： C12N5/00 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/53 ,  A61K2039/552 ,  A61K2039/70 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10043 ,  C12Q1/701

摘要： The cloning of a novel PCVII viral genome is described as is expression of proteins derived from the PCVII genome. These proteins can be used in vaccine compositions for the prevention and treatment of PCVII infections, as well as in diagnostic methods for determining the presence of PCVII infections in a vertebrate subject. Polynucleotides derived from the viral genome can be used as diagnostic primers and probes.

公开(公告)号：US06517843B1

公开(公告)日：2003-02-11

申请号：US09583350

申请日：2000-05-31

申请人： John Albert Ellis ,  Gordon Allan Moore ,  Brian Meehan ,  Edward Clark ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  George Steve Krakowka ,  Jean-Christophe Francis Audonnet ,  Francis McNeilly

发明人： John Albert Ellis ,  Gordon Allan Moore ,  Brian Meehan ,  Edward Clark ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  George Steve Krakowka ,  Jean-Christophe Francis Audonnet ,  Francis McNeilly

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A61K2039/53 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/24043 ,  C12N2750/10022

摘要： Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6th and 8th and/or the 10th and 13th weeks of gestation, can prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and/or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

摘要翻译： 猪圆环病毒-2（PCV-2）是最近确定的药物，其中PCV-2相关疾病的潜在谱已经通过垂直和性传播的证据以及猪群体相关的生殖失败而扩大。 从经历了晚期堕胎和死胎的农场的排卵仔猪中分离出PCV-2。 在与PCV-2抗原的广泛免疫组织化学染色相关的一只仔猪中存在严重的弥漫性心肌炎。 不同量的PCV-2抗原也存在于多个胎儿的肝，肺和肾中。 包括来自PCV-2或感兴趣的表位的免疫原的组合物的雌性猪从这样的免疫原或在繁殖之前（例如在生命的前五周内）表达这种免疫原或感兴趣的表位的载体接种，或 在围产期，或一生以上，或怀孕期间，如6〜8和/或10〜13周的妊娠期间，可以预防与猪圆环病毒-2相关的心肌炎，流产和子宫内感染。 此外，可以进行具有上述组成的雄性和/或雌性猪的接种，以防止在交配期间PCV-2从雄性到雌性（或反之亦然）的传播。 因此，本发明涉及用于预防与猪圆环病毒-2相关的心肌炎，流产和子宫内感染的方法和组合物。

公开(公告)号：US06391314B1

公开(公告)日：2002-05-21

申请号：US09161092

申请日：1998-09-25

申请人： Gordon Allan ,  Brian Meehan ,  Edward Clark ,  John Ellis ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly

发明人： Gordon Allan ,  Brian Meehan ,  Edward Clark ,  John Ellis ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/5252 ,  A61K2039/552 ,  A61K2039/55566 ,  C12N2710/10343 ,  C12N2710/16043 ,  C12N2710/24043 ,  C12N2750/10021 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10043 ,  C12N2750/10063 ,  C12N2750/10064

摘要： The invention relates to new porcine circovirus strains isolated from pulmonary or ganglionic samples obtained from farms affected by the post-weaning multisystemic wasting syndrome (PMWS). It relates to purified preparations of these strains, conventional attenuated or inactivated vaccines, recombinant live vaccines, plasmid vaccines and subunit vaccines, as well as reagents and diagnostic methods. It also relates to the DNA fragments which can be used for the production of subunits in an in vitro expression vector or as sequences to be integrated into a virus or plasmid type in vivo expression vector.

摘要翻译： 本发明涉及从受断乳多系统消耗综合征（PMWS）影响的农场获得的肺或神经节样品中分离的新的猪圆环病毒。 它涉及这些菌株的纯化制剂，常规减毒或灭活疫苗，重组活疫苗，质粒疫苗和亚单位疫苗，以及试剂和诊断方法。 它还涉及可用于在体外表达载体中产生亚单位的DNA片段或作为整合到病毒或质粒体内表达载体中的序列的DNA片段。

公开(公告)号：US6096535A

公开(公告)日：2000-08-01

申请号：US196187

申请日：1998-11-20

申请人： Raphael Darteil ,  Wayne Corapi ,  Jean-Christophe Fran.cedilla.is Audonnet ,  Gilles Emile Chappuis

发明人： Raphael Darteil ,  Wayne Corapi ,  Jean-Christophe Fran.cedilla.is Audonnet ,  Gilles Emile Chappuis

IPC分类号： C12N15/09 ,  A61K39/00 ,  A61K39/215 ,  A61P31/12 ,  C07K14/155 ,  C07K14/165 ,  C12N15/50

CPC分类号： C07K14/005 ,  G06F19/3406 ,  A61K2039/51 ,  A61K2039/525 ,  A61K39/00 ,  C12N2710/24143 ,  C12N2740/15022 ,  C12N2770/20022

摘要： The invention comprises the nucleotide sequences comprising the FIPV S gene, or a fragment of this gene, which are modified in at least one of the antigenic regions A1 and A2 which are involved in enhancement, as well as the use of these sequences for the expression of modified proteins, and for the construction of recombinant viruses or expression plasmids, and the use of the recombinant viruses as vaccines against feline infectious peritonitis, the use of the expression plasmids as immunizing composition by direct injection of the said plasmids into cats, and the use of the modified proteins as vaccine.

公开(公告)号：US5665534A

公开(公告)日：1997-09-09

申请号：US417276

申请日：1995-04-05

申请人： Danielle Marie Helene Jeanne Vandenbergh ,  Corine Martine Therese Ghislaine LeComte ,  Gilles-Emile Chappuis ,  Jean-Jacques Pin

发明人： Danielle Marie Helene Jeanne Vandenbergh ,  Corine Martine Therese Ghislaine LeComte ,  Gilles-Emile Chappuis ,  Jean-Jacques Pin

IPC分类号： C07K16/00 ,  C07K14/18 ,  C07K14/435 ,  C12N1/19 ,  C12N5/10 ,  C12N7/00 ,  C12N15/09 ,  C12N15/40 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/70 ,  C12R1/865 ,  C12R1/91 ,  C12R1/92 ,  G01N33/53 ,  G01N33/569

CPC分类号： C07K14/005 ,  G01N33/56983 ,  C12N2770/24322

摘要： The invention is directed to a method of detecting infection of a blood sample by various strains of bovine diarrhoea virus (BVD) including a first test wherein anti-bovine diarrhoea virus antibodies are detected by means of a recombinant antigen comprising the BVD p80 protein expressed by a eukaryotic host and a second test wherein viral particles are detected by means of antibodies directed against the BVD p80 viral protein. The method is particularly useful in detecting pathogenic conditions such as persistent viremias and acute infections caused by BVD viruses, and is particularly advantageous because it is an extremely sensitive and efficient test and because it is capable of detecting infections caused by any one of a wide variety of BVD strains. The recombinant antigen employed in the method is preferably encoded by the nucleotide sequence listed as SEQ ID No: 1.

摘要翻译： 本发明涉及一种通过各种牛腹泻病毒（BVD）检测血液样品感染的方法，该方法包括第一试验，其中通过包含由BVD p80蛋白表达的重组抗原检测抗牛腹泻病毒抗体 真核宿主和第二次测试，其中通过针对BVD p80病毒蛋白的抗体检测病毒颗粒。 该方法特别可用于检测诸如持续性病毒血症和由BVD病毒引起的急性感染等病原性病症，并且是特别有利的，因为它是非常敏感和有效的测试，并且因为它能够检测由各种各样的任何一种引起的感染 的BVD株。 该方法中使用的重组抗原优选由SEQ ID No：1列出的核苷酸序列编码。

公开(公告)号：US07803926B2

公开(公告)日：2010-09-28

申请号：US11522237

申请日：2006-09-15

申请人： Deborah Haines ,  Gordon Allan ,  John Ellis ,  Brian Meehan ,  Edward Clark ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly ,  Li Wang ,  Lorne A. Babiuk ,  Andrew A. Potter ,  Philip Willson

发明人： Deborah Haines ,  Gordon Allan ,  John Ellis ,  Brian Meehan ,  Edward Clark ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly ,  Li Wang ,  Lorne A. Babiuk ,  Andrew A. Potter ,  Philip Willson

IPC分类号： C12N15/00 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/53 ,  A61K2039/552 ,  A61K2039/70 ,  C12N7/00 ,  C12N15/86 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10043 ,  C12Q1/701

摘要： The cloning of a novel PCVII viral genome is described as is expression of proteins derived from the PCVII genome. These proteins can be used in vaccine compositions for the prevention and treatment of PCVII infections, as well as in diagnostic methods for determining the presence of PCVII infections in a vertebrate subject. Polynucleotides derived from the viral genome can be used as diagnostic primers and probes.

公开(公告)号：US07211379B2

公开(公告)日：2007-05-01

申请号：US10780318

申请日：2004-02-17

申请人： John Ellis ,  Gordon Moore Allan ,  Brian Meehan ,  Edward Clark ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  George Steve Krakowka ,  Jean-Christophe Francis Audonnet ,  Francis McNeilly

发明人： John Ellis ,  Gordon Moore Allan ,  Brian Meehan ,  Edward Clark ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  George Steve Krakowka ,  Jean-Christophe Francis Audonnet ,  Francis McNeilly

IPC分类号： C12Q1/70 ,  C07H21/04 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/552 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/16743 ,  C12N2710/24043 ,  C12N2750/10022 ,  C12N2750/10034

摘要： Provided is a method for reducing viral load of porcine circovirus type 2 (PCV-2) in a pig by inducing an immune response against PCV-2 through the administration of an immunogenic composition comprising a PCV-2 antigen. A preferred antigen is a vector containing a PCV-2 nucleotide sequence. In a particularly preferred embodiment, the PCV-2 nucleotide sequence is ORF4, ORF13, or ORF4 and ORF13. In some embodiments, the immunogenic composition includes one or more additional pig pathogens.

摘要翻译： 本发明提供了一种通过通过施用包含PCV-2抗原的免疫原性组合物诱导针对PCV-2的免疫应答来降低猪圆环病毒2型（PCV-2）的病毒载量的方法。 优选的抗原是含有PCV-2核苷酸序列的载体。 在特别优选的实施方案中，PCV-2核苷酸序列是ORF4，ORF13或ORF4和ORF13。 在一些实施方案中，免疫原性组合物包括一种或多种另外的猪病原体。

公开(公告)号：US07122192B2

公开(公告)日：2006-10-17

申请号：US10624049

申请日：2003-07-21

申请人： Gordon Allan ,  Brian Meehan ,  Edward Clark ,  John Ellis ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly

发明人： Gordon Allan ,  Brian Meehan ,  Edward Clark ,  John Ellis ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly

IPC分类号： A61K39/12

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/5252 ,  A61K2039/552 ,  A61K2039/55566 ,  C12N2710/10343 ,  C12N2710/16043 ,  C12N2710/24043 ,  C12N2750/10021 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10043 ,  C12N2750/10063 ,  C12N2750/10064

摘要： The invention relates to new porcine circovirus strains isolated from pulmonary or ganglionic samples obtained from farms affected by the post-weaning multisystemic wasting syndrome (PMWS). It relates to purified preparations of these strains, conventional attenuated or inactivated vaccines, recombinant live vaccines, plasmid vaccines and subunit vaccines, as well as reagents and diagnostic methods. It also relates to the DNA fragments which can be used for the production of subunits in an in vitro expression vector or as sequences to be integrated into a virus or plasmid type in vivo expression vector.

公开(公告)号：US06368601B1

公开(公告)日：2002-04-09

申请号：US09082558

申请日：1998-05-21

申请人： Gordon Allan ,  Brian Meehan ,  Edward Clark ,  John Ellis ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly

发明人： Gordon Allan ,  Brian Meehan ,  Edward Clark ,  John Ellis ,  Deborah Haines ,  Lori Hassard ,  John Harding ,  Catherine Elisabeth Charreyre ,  Gilles Emile Chappuis ,  Francis McNeilly

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A23C19/0321 ,  A23C19/0323 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K48/00 ,  A61K2039/51 ,  A61K2039/5252 ,  A61K2039/552 ,  A61K2039/55566 ,  C12N2750/10021 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10043

摘要： The invention relates to new porcine circovirus strains isolated from pulmonary or ganglionic samples obtained from farms affected by the post-weaning multisystemic wasting syndrome (PMWS). It relates to purified preparations of these strains, conventional attenuated or inactivated vaccines, recombinant live vaccines, plasmid vaccines and subunit vaccines, as well as reagents and diagnostic methods. It also relates to the DNA fragments which can be used for the production of subunits in an in vitro expression vector or as sequences to be integrated into a virus or plasmid type in vivo expression vector.

摘要翻译： 本发明涉及从受断乳多系统消耗综合征（PMWS）影响的农场获得的肺或神经节样品中分离的新的猪圆环病毒。 它涉及这些菌株的纯化制剂，常规减毒或灭活疫苗，重组活疫苗，质粒疫苗和亚单位疫苗，以及试剂和诊断方法。 它还涉及可用于在体外表达载体中产生亚单位的DNA片段或作为整合到病毒或质粒体内表达载体中的序列的DNA片段。