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    Expression system incorporating a capsid promoter sequence as an enhancer
    1.
    发明授权
    Expression system incorporating a capsid promoter sequence as an enhancer 有权
    包含衣壳启动子序列作为增强子的表达系统

    公开(公告)号：US08460933B2

    公开(公告)日：2013-06-11

    申请号：US12084728

    申请日：2006-11-08

    申请人： Edward Peter Rybicki ,  Fiona Lesley Tanzer

    发明人： Edward Peter Rybicki ,  Fiona Lesley Tanzer

    IPC分类号： C12N15/67 ,  C12N15/85 ,  C12N5/16 ,  A61K48/00

    CPC分类号： C12N15/85 ,  A61K2039/53 ,  C12N2750/10043 ,  C12N2830/00 ,  C12N2830/30 ,  C12N2830/42 ,  C12N2830/60

    摘要： The invention provides a method for enhancing expression of a transgene in a host cell, which includes the steps of inserting a capsid promoter element (Pcap), or a sequence comprising the reverse complement of the capsid promoter element (PcapR), into a mammalian expression cassette upstream (5′) of a cytomegalovirus immediate/early enhancer/promoter region (Pcmv); inserting the transgene into the expression cassette downstream (3′) of the cytomegalovirus immediate/early enhancer/promoter region; inserting a vector containing the expression cassette into the host organism; and causing expression of the transgene. The capsid promoter element (Pcap) is typically from a circovirus, parvovirus or anellovirus. The transgene is typically expressed at a higher level than when expressed by a vector containing the expression cassette without the transcriptional control element. An expression cassette, vector, DNA vaccine, pharmaceutical composition and method of treatment are also claimed.

    摘要翻译： 本发明提供一种增强宿主细胞中转基因表达的方法，其包括将衣壳启动子元件（Pcap）或包含衣壳启动子元件（PcapR）的反向互补序列的序列插入到哺乳动物表达中的步骤 巨细胞病毒立即/早期增强子/启动子区（Pcmv）上游（5'）的盒; 将转基因插入巨细胞病毒立即/早期增强子/启动子区域下游（3'）的表达盒中; 将含有表达盒的载体插入宿主生物体中; 并引起转基因的表达。 衣壳启动子元件（Pcap）通常来自圆环病毒，细小病毒或anellovirus。 转录子通常以比不含转录控制元件的含有表达盒的载体表达的水平更高的水平表达。 还要求保护表达盒，载体，DNA疫苗，药物组合物和治疗方法。