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    Methods for producing a polypeptide using a consensus translational initiator sequence
    1.
    发明授权
    Methods for producing a polypeptide using a consensus translational initiator sequence 有权
    使用共有翻译起始序列产生多肽的方法

    公开(公告)号：US06461837B1

    公开(公告)日：2002-10-08

    申请号：US09717847

    申请日：2000-11-20

    申请人： Debbie S. Yaver ,  Daniel Alan Bellini

    发明人： Debbie S. Yaver ,  Daniel Alan Bellini

    IPC分类号： C12P2106

    CPC分类号： C12N15/80 ,  C07K2319/00 ,  C12N9/20 ,  C12N15/67

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a consensus translational initiator sequence foreign to the nucleic acid sequence; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated consensus translational initiator sequences and to constructs, vectors, and fungal host cells comprising the consensus translational initiator sequences operably linked to nucleic acid sequences encoding polypeptides.

    摘要翻译： 本发明涉及生产多肽的方法，其包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含编码多肽的第一核酸序列，其可操作地连接到 第二核酸序列，其包含与所述核酸序列不同的共有翻译起始序列; 和（b）从培养基中分离多肽。 本发明还涉及分离的共有翻译起始序列和构建体，载体和真菌宿主细胞，其包含与编码多肽的核酸序列可操作地连接的共有翻译起始序列。

    Coronamycins
    6.
    发明授权
    Coronamycins 失效
    冠心病

    公开(公告)号：US07387888B2

    公开(公告)日：2008-06-17

    申请号：US11064486

    申请日：2005-02-24

    申请人： Gary A. Strobel ,  Debbie S. Yaver ,  Uvidelio F. Castillo ,  David Ezra

    发明人： Gary A. Strobel ,  Debbie S. Yaver ,  Uvidelio F. Castillo ,  David Ezra

    IPC分类号： C12C1/00

    CPC分类号： C12R1/465 ,  A01N63/00 ,  A01N63/02 ,  A61K38/00 ,  C07K14/36 ,  C12P21/00

    摘要： The present invention relates to isolated strains of a Streptomyces spp. which are endophytes of dicotyledonous plants and to methods for selecting such strains. The present invention also relates to a biologically active compound called coronamycin obtained from endophytic Streptomycetes isolated from higher plants. The present invention further relates to compositions of such compounds and to methods of protecting plants against attack by a plant pathogen and methods of inhibiting bacterial growth, fungal growth, viral infection, growth of parasitic organisms, and cancer cell growth with such compositions.

    摘要翻译： 本发明涉及链霉菌属（Streptomyces spp。）的分离菌株。 其是双子叶植物的内生真菌和选择这些菌株的方法。 本发明还涉及从高等植物分离的内生菌链球菌获得的称为冠状病毒的生物活性化合物。 本发明进一步涉及这些化合物的组合物以及保护植物免受植物病原体攻击的方法以及用这些组合物抑制细菌生长，真菌生长，病毒感染，寄生生物生长和癌细胞生长的方法。

    Methods for isolating genes from microorganisms
    7.
    发明授权
    Methods for isolating genes from microorganisms 失效
    从微生物分离基因的方法

    公开(公告)号：US06942973B2

    公开(公告)日：2005-09-13

    申请号：US10095975

    申请日：2002-03-12

    申请人： Debbie S. Yaver ,  Randy M. Berka

    发明人： Debbie S. Yaver ,  Randy M. Berka

    IPC分类号： C12N15/10 ,  C12N15/30 ,  C12N15/52 ,  C12N15/74 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/08 ,  C12P21/02

    CPC分类号： C12Q1/6895 ,  C12N15/1034 ,  C12Q1/6809 ,  C12Q2600/158 ,  C12Q2565/501 ,  C12Q2565/102 ,  C12Q2525/179

    摘要： The present invention relates to methods for isolating a gene encoding an enzyme, comprising: (a) adding a mixture of labeled first nucleic acid probes from a microbial strain cultured on medium without the substrate, and labeled second nucleic acid probes from a microbial strain cultured on medium with the substrate, to an array of random nucleic acid fragments of the microbial strain where the labeled nucleic acids hybridize to complementary sequences of the genomic fragments in the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter; (b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot in the array; and (c) isolating a gene from the microbial strain that encodes an enzyme that degrades the substrate. The present invention also relates to isolated genes obtained by such methods.

    摘要翻译： 本发明涉及用于分离编码酶的基因的方法，其包括：（a）从培养基上培养的微生物菌株中加入标记的第一核酸探针的混合物，而不含底物，以及来自培养的微生物菌株的标记的第二核酸探针 在具有底物的培养基上的微生物菌株的随机核酸片段的阵列，其中标记的核酸与阵列中的基因组片段的互补序列杂交，其中第一核酸探针用第一报道分子标记， 核酸探针用第二报告物标记; （b）在条件下检查阵列，其中微生物菌株的基因的相对表达通过阵列中每个斑点的观察杂交报道信号确定; 和（c）从编码降解底物的酶的微生物菌株中分离基因。 本发明还涉及通过这些方法获得的分离基因。

    Methods for producing a polypeptide using a crippled translational initiator sequence
    8.
    发明授权
    Methods for producing a polypeptide using a crippled translational initiator sequence 失效

    公开(公告)号：US06548274B2

    公开(公告)日：2003-04-15

    申请号：US09760139

    申请日：2001-01-12

    申请人： Debbie S. Yaver ,  Daniel A. Bellini

    发明人： Debbie S. Yaver ,  Daniel A. Bellini

    IPC分类号： C12N1500

    CPC分类号： C12N15/905 ,  C12N15/80

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide; and (b) isolating the polypeptide from the cultivation medium; wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide in tandem with a second nucleic acid sequence comprising a crippled translational initiator sequence operably linked to a gene encoding a selectable marker, wherein the copy number of the first nucleic acid sequence has been increased by culturing the cell under conditions that select for multiple copies of the selectable marker. The present invention also relates to such fungal host cells and methods for obtaining such fungal host cells. The present invention further relates to nucleic acid constructs and vectors comprising a crippled translational initiator sequence operably linked to a gene encoding a selectable marker.