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26 条记录 (耗时 0.026 秒)

    Expression of herbicide tolerance genes in plant plastids
    4.
    发明授权
    Expression of herbicide tolerance genes in plant plastids 失效
    除草剂耐受基因在植物质体中的表达

    公开(公告)号：US06492578B1

    公开(公告)日：2002-12-10

    申请号：US09351123

    申请日：1999-07-10

    申请人： Jeffrey M. Staub ,  Peter Hajdukiewicz ,  Kevin E. McBride ,  Narender Nehra ,  David J. Schaaf ,  David M. Stalker ,  Guangning Ye

    发明人： Jeffrey M. Staub ,  Peter Hajdukiewicz ,  Kevin E. McBride ,  Narender Nehra ,  David J. Schaaf ,  David M. Stalker ,  Guangning Ye

    IPC分类号： C12N1582

    CPC分类号： C12N9/1092 ,  C07K2319/00 ,  C12N9/14 ,  C12N9/88 ,  C12N15/8214 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8278

    摘要： Provided are constructs and methods for expressing herbicide tolerance genes in plastids of plant cells. Constructs include the components of a promoter functional in a plant plastid, a DNA sequence which is capable of conferring tolerance in a plant cell to at least one herbicide compound when said DNA sequence is transcribed in plastids of said plant cell and a transcription termination region. Herbicide tolerance is produced by transforming plastids with the constructs of the invention and growing plant cells comprising the transformed plastids under conditions wherein the DNA sequence is transcribed and plant plastids and cells containing the plastids are rendered tolerant to applications of at least one herbicide compound.

    摘要翻译： 提供了用于在植物细胞的质体中表达除草剂耐受基因的构建体和方法。 构建体包括在植物质体中起作用的启动子的组分，当所述DNA序列在所述植物细胞的质体和转录终止区中转录时，能够赋予植物细胞对至少一种除草剂化合物的耐受性的DNA序列。 通过用本发明的构建体转化质体和包含转化的质体的条件下生长的植物细胞产生除草剂耐受性，其中DNA序列被转录并且植物质体和包含质体的细胞对至少一种除草剂化合物的施用具有耐受性。

    Controlled expression of transgenic constructs in plant plastids
    6.
    发明授权
    Controlled expression of transgenic constructs in plant plastids 失效
    植物质体中转基因构建体的受控表达

    公开(公告)号：US5925806A

    公开(公告)日：1999-07-20

    申请号：US472719

    申请日：1995-06-06

    申请人： Kevin E. McBride ,  David M. Stalker

    发明人： Kevin E. McBride ,  David M. Stalker

    IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N5/14 ,  C12N15/33 ,  C12N15/52

    CPC分类号： C12N15/8217 ,  C12N15/8214 ,  C12N15/8216

    摘要： A novel compositions and methods useful for genetic engineering of plant cells to provide a method of controlling the timing or tissue pattern of expression of foreign DNA sequences inserted into the plant plastid genome are provided in the instant invention. Constructs include those for nuclear transformation which provide for expression of a viral single subunit RNA polymerase in plant cell tissues, and targeting of the expressed polymerase protein into the plant cell plastids. In addition, plastid expression constructs comprising a viral gene promoter region which is specific to the RNA polymerase expressed from the nuclear expression constructs described above, and a DNA sequence of interest to be expressed in the transformed plastid cells are provided. Plant cells and plants comprising the nuclear and/or plastid constructs described herein are of interest. Of particular interest is a method of controlling expression of the inserted plastid gene constructs in a tissue and/or developmental specific manner in plants comprising both the nuclear polymerase construct and the plastid expression constructs.

    摘要翻译： 在本发明中提供了一种用于植物细胞的遗传工程的新型组合物和方法，以提供控制插入植物质体基因组中的外来DNA序列的表达的时间或组织模式的方法。 构建体包括提供用于在植物细胞组织中表达病毒单亚基RNA聚合酶的核转化的构建体，以及将表达的聚合酶蛋白靶向植物细胞质体。 此外，提供了包含由上述核表达构建体表达的RNA聚合酶特异的病毒基因启动子区和在转化的质体细胞中表达的目标DNA序列的质体表达构建体。 包含本文所述的核和/或质体构建体的植物细胞和植物是令人感兴趣的。 特别感兴趣的是在包含核聚合酶构建体和质体表达构建体的植物中以组织和/或发育特异性方式控制插入的质体基因构建体的表达的方法。

    Use of ovary-tissue transcriptional factors for altering plant color
    9.
    发明授权
    Use of ovary-tissue transcriptional factors for altering plant color 失效
    使用卵巢组织转录因子改变植物的颜色

    公开(公告)号：US06222097B1

    公开(公告)日：2001-04-24

    申请号：US08984320

    申请日：1997-12-03

    申请人： Kevin E. McBride ,  David M. Stalker

    发明人： Kevin E. McBride ,  David M. Stalker

    IPC分类号： A01H100

    CPC分类号： C07K14/415 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/0071 ,  C12N15/8218 ,  C12N15/8222 ,  C12N15/8233 ,  C12N15/825

    摘要： Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest in ovary tissue, particularly in very early fruit development. The DNA constructs comprise a transcriptional initiation regulatory region associated with gene expression in ovary tissue from immediately prior to anthesis through flower senescence.

    摘要翻译： 提供了新的DNA构建体，其可以用作分子探针或插入到植物宿主中以提供对于在卵巢组织中感兴趣的DNA序列的转录的修饰，特别是在非常早期的果实发育中。 DNA构建体包含与通过花衰老开花之前的卵巢组织中与基因表达相关的转录起始调节区。

    Enhanced expression in a plant plastid
    10.
    发明授权
    Enhanced expression in a plant plastid 失效
    在植物质体中增强表达

    公开(公告)号：US5866421A

    公开(公告)日：1999-02-02

    申请号：US593205

    申请日：1996-01-29

    申请人： Kevin E. McBride ,  David M. Stalker

    发明人： Kevin E. McBride ,  David M. Stalker

    IPC分类号： A01H5/00 ,  C07K14/325 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82 ,  C12P21/02 ,  C12N5/14

    CPC分类号： C12N15/8286 ,  C07K14/325 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8223

    摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.

    摘要翻译： 用于植物细胞遗传工程的新型组合物和方法可用于提供蛋白质的植物或植物细胞的质体中增加的表达，该蛋白质产生当在不存在选择转化的方法的情况下生长植物或植物细胞时存在的表型 细胞。 苏云金芽孢杆菌细菌原毒素在植物叶绿体中的表达。