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    Extracellular vesicles comprising engineered fusion proteins
    2.
    发明授权
    Extracellular vesicles comprising engineered fusion proteins 有权

    公开(公告)号：US11578116B2

    公开(公告)日：2023-02-14

    申请号：US17377550

    申请日：2021-07-16

    申请人： DIADEM BIOTHERAPEUTICS INC.

    发明人： Mickey Pentecost ,  Wojciech Bartkowski

    IPC分类号： C07K14/705 ,  A61K47/69 ,  A61K47/58 ,  A61K47/62 ,  A61K47/54 ,  A61P27/02 ,  A61P37/06 ,  C12N9/14 ,  C07K14/75 ,  A61K38/00

    摘要： Described herein are compositions and techniques related to generation and therapeutic application of artificial synapses. Artificial synapses are engineered extracellular vesicles, including exosomes, which incorporate sticky binders on their surface to anchor signaling domains against biological targets, such as receptors. These engineered additives can be organized in genetic vector constructs, expressed in mammalian cells, wherein the sticky binders attach to extracellular vesicles such as exosomes, thereby presenting their joined signaling domains which are rapidly taken up by recipient cells. Artificial synapses adopt the hallmark biophysical and biochemical features of extracellular vesicles, allowing for rapid deployment and scale-up. Importantly, this strategy can allow for kinetically favorable signal generation and signal propagation. This includes, for example, increasing density of agonist presentation to support receptor clustering—an onerous barrier for traditional receptor targeting strategies.

    METHOD FOR PRODUCING FIBRIN SHEET
    3.
    发明申请
    METHOD FOR PRODUCING FIBRIN SHEET 有权

    公开(公告)号：US20220372437A1

    公开(公告)日：2022-11-24

    申请号：US17765637

    申请日：2020-09-09

    申请人： OSAKA UNIVERSITY

    发明人： Takaaki SAMURA ,  Shigeru MIYAGAWA ,  Yoshiki SAWA ,  Junya YOKOYAMA

    IPC分类号： C12N5/00 ,  C07K14/75

    摘要： A method for producing a fibrin sheet containing at least one selected from the group consisting of cells and drugs in a fibrin gel, the method comprising: a step 1 of applying a fibrinogen solution containing at least one selected from the group consisting of cells and drugs and fibrinogen dropwise onto a surface of a substrate made of a gelatin hydrogel; a step 2 of adding thrombin to the fibrinogen solution on the surface of the substrate; a step 3 of placing a support film on and in contact with a top surface of the fibrinogen solution to which the thrombin has been added; a step 4 of forming a fibrin sheet containing the at least one selected from the group consisting of cells and drugs in a fibrin gel between the substrate and the support film by a reaction between the fibrinogen and the thrombin; and a step 5 of melting the substrate at a temperature not lower than a melting temperature of the gelatin hydrogel to separate, from the substrate, the fibrin sheet supported by the support film.

    Fibrinogen-based tissue adhesive patches
    4.
    发明授权
    Fibrinogen-based tissue adhesive patches 有权

    公开(公告)号：US11071805B2

    公开(公告)日：2021-07-27

    申请号：US16041903

    申请日：2018-07-23

    申请人： SEALANTIUM MEDICAL LTD.

    发明人： Orgad Laub ,  Daniel Cohn ,  Eran Cohen ,  Matthew Zarek

    IPC分类号： A61L15/16 ,  A61L15/32 ,  C07K14/75 ,  A61L27/22 ,  A61L27/60 ,  A61L27/36 ,  A61L27/58 ,  C09J175/04 ,  A61L27/18 ,  A61F2/10

    摘要： A novel fibrinogen-based tissue adhesive patch is disclosed. The patch comprises a backing made from a non-permeable biocompatible polymer film into which a fibrinogen-based sealant is incorporated. In preferred embodiments of the invention, the biocompatible polymer film comprises units of a biocompatible block copolymer such as a polyethylene glycol-polycaprolactone-DL-lactide copolymer connected by urethane linkages, and the fibrinogen-based sealant comprises fibrinogen, thrombin, and CaCl2. In contrast to similar patches known in the art, the polymer backing serves to seal the tissue to which the patch is applied, and the sealant acts only to bind the patch to the affected tissue. The patch does not include any mesh, woven, or non-woven component. Methods of production and use of the patch are also disclosed.