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公开(公告)号：US08686225B2
公开(公告)日：2014-04-01
申请号：US13449233
申请日：2012-04-17
CPC分类号： G01N33/57492 , A23K10/30 , A23K20/147 , A23L7/198 , A23L7/20 , A23L33/105 , A23L33/19 , A23V2002/00 , B82Y30/00 , C07K14/415 , C07K14/485 , C07K14/65 , C07K14/79 , C07K14/8125 , C12N9/0065 , C12N9/2462 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8257 , G01N33/57484 , G01N2333/4725 , G01N2500/02 , A23V2300/21
摘要： The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, as well as to methods of making these isolated blood proteins and to therapeutic compositions comprising them.
摘要翻译： 本发明涉及在单子叶植物中产生并从其分离用于治疗组合物中的血液蛋白质，以及制备这些分离的血液蛋白质的方法以及包含它们的治疗组合物。
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公开(公告)号：US06288303B1
公开(公告)日：2001-09-11
申请号：US09105390
申请日：1998-06-25
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
IPC分类号： C12N1529
CPC分类号： C12N15/8222 , C07K14/8125 , C12N9/244 , C12N15/8223 , C12N15/8227 , C12N15/8234 , C12N15/8237 , C12N15/8257 , C12N15/8282 , C12Y302/01006
摘要： A group of novel rice &bgr;-glucanase genes, identified as &bgr;-glucanases 2-9 (Gns 2-9), and the corresponding &bgr;-glucanase enzymes, are disclosed. The genes, and the gene promoters, are useful in a variety of transgenic monocot plants, for achieving increased plant resistance to fungal infection, improved growth characteristics, and high levels of expression of heterologous protein in various tissues obtained from the plants.
摘要翻译： 公开了一组鉴定为β-葡聚糖酶2-9（Gns 2-9）的新型水稻β-葡聚糖酶基因和相应的β-葡聚糖酶。 基因和基因启动子可用于各种转基因植物单体植物，用于实现增加的植物对真菌感染的抗性，改善的生长特性以及从植物获得的各种组织中异源蛋白质的高水平表达。
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公开(公告)号：US6127145A
公开(公告)日：2000-10-03
申请号：US23339
申请日：1998-02-13
CPC分类号： C12N15/8257 , C12N15/8234 , C12N15/8235
摘要： A method for producing .alpha..sub.1 -antitrypsin (AAT) in plant cells is disclosed. Monocot plant cells transformed with an AAT coding sequence are cultivated under conditions efficient for protein expression and secretion. Also disclosed are a codon-optimized AAT coding sequence that is efficiently translated in plant cell culture and a novel AAT protein having the glycosylation pattern characteristic of plant cells and suitable for therapeutic use in humans.
摘要翻译： 公开了一种在植物细胞中产生α1-抗胰蛋白酶（AAT）的方法。 用AAT编码序列转化的单子叶植物细胞在对蛋白质表达和分泌有效的条件下培养。 还公开了在植物细胞培养物中有效翻译的密码子优化的AAT编码序列和具有植物细胞的糖基化模式特征并且适合于人类治疗用途的新型AAT蛋白质。
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公开(公告)号：US07417178B2
公开(公告)日：2008-08-26
申请号：US10639835
申请日：2003-08-12
申请人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
发明人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
CPC分类号： G01N33/57492 , A23K10/30 , A23K20/147 , A23L7/198 , A23L7/20 , A23L33/105 , A23L33/19 , A23V2002/00 , B82Y30/00 , C07K14/415 , C07K14/485 , C07K14/65 , C07K14/79 , C07K14/8125 , C12N9/0065 , C12N9/2462 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8257 , G01N33/57484 , G01N2333/4725 , G01N2500/02 , A23V2300/21
摘要： The invention is directed to seed and seed extract compositions containing levels of a human milk protein between 3-40% or higher of the total protein weight of the soluble protein extractable from the seed. Also disclosed is a method of producing the seed with high levels of extractable human milk protein. The method includes transforming a monocotyledonous plant with a chimeric gene having a protein-coding sequence encoding a protein normally present in human milk under the control of a seed maturation-specific promoter. The method may further includes a leader DNA sequence encoding a monocot seed-specific transit sequence capable to target a linked milk protein to a storage body.
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公开(公告)号：US06680425B1
公开(公告)日：2004-01-20
申请号：US09450515
申请日：1999-11-29
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
IPC分类号： C12N504
CPC分类号： C12N9/2422 , C12N15/8238
摘要： A chimeric plant promoter which is responsive to sugar depletion or deprivation is disclosed. The promoter includes a promoter element and, carried in the element, a heterologous sequence from the rice Amy3D that is responsive to sugar depletion. Also disclosed is a plant transfecting vector containing the sequence, and plant cell transfected with the vector.
摘要翻译： 公开了对糖消耗或剥夺有反应的嵌合植物启动子。 启动子包括启动子元件，并且在元件中携带来自对糖消耗有反应的稻米Amy3D的异源序列。 还公开了含有该序列的植物转染载体和用载体转染的植物细胞。
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公开(公告)号：US06284956B1
公开(公告)日：2001-09-04
申请号：US09344438
申请日：1999-06-25
申请人： Raymond L Rodriguez , Ning Huang
发明人： Raymond L Rodriguez , Ning Huang
IPC分类号： C12N1529
CPC分类号： C12N15/8222 , C12N9/1029 , C12N9/1205 , C12N9/2422 , C12N15/8201 , C12N15/8207 , C12N15/8209 , C12N15/8234 , C12N15/8242 , C12N15/8257 , C12Y203/01183 , C12Y302/01001
摘要： A plant transformation expression cassette for transforming host plant cells with a selectable marker gene is disclosed. The cassette includes, operatively linked in sequence in a 5′ to 3′ direction, (i) a DNA promoter sequence from the rice beta-glucanase 9 (gns9) gene; (ii) a selectable marker gene, and (iii) a 3′ untranslated terminator region. Also disclosed are a expression cassette pair containing the transformation vector, a method of obtaining transformed monocots whose seeds produce a selected heterologous protein during sed germination, and a plant whose cells are transformed with the chimeric selectable marker gene.
摘要翻译： 公开了用选择性标记基因转化宿主植物细胞的植物转化表达盒。 盒子包括按照5'到3'方向顺序连接的顺序，（i）来自水稻β-葡聚糖酶9（gns9）基因的DNA启动子序列; （ii）选择标记基因，和（iii）3'非翻译终止子区。 还公开了含有转化载体的表达盒对，在种子发芽期间获得种子产生选择的异源蛋白质的转化单子叶植物的方法，以及用嵌合选择标记基因转化其细胞的植物。
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公开(公告)号：US4588689A
公开(公告)日：1986-05-13
申请号：US585752
申请日：1984-03-02
CPC分类号： C12N9/22 , Y10S435/91
摘要： A novel restriction endonuclease designated XcyI recognizes and cleaves the sequence 5'-C.dwnarw.CCGGG-3', where the arrow indicates the cleavage site. The enzyme may be obtained from Xanthamonas cyanopsidis.Xanthamonas cyanopsidis strain 13D5 was deposited at the American Type Culture Collection on Jan. 20, 1984, and granted accession No. 39587.
摘要翻译： 称为XcyI的新型限制性内切核酸酶识别并切割序列5'-C和darr＆CCGGG-3'，其中箭头表示切割位点。 该酶可以从蓝假单胞菌（Xanthamonas cyanopsidis）获得。 蓝假丝酵母菌株13D5于1984年1月20日保藏于美国典型培养物保藏中心，授予加拿社第39587号。
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公开(公告)号：US06991824B2
公开(公告)日：2006-01-31
申请号：US10077381
申请日：2002-02-14
申请人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
发明人： Ning Huang , Raymond L. Rodriguez , Frank E. Hagie
IPC分类号： A21D2/26
CPC分类号： G01N33/57492 , A23K10/30 , A23K20/147 , A23L7/198 , A23L7/20 , A23L33/105 , A23L33/19 , A23V2002/00 , B82Y30/00 , C07K14/415 , C07K14/485 , C07K14/65 , C07K14/79 , C07K14/8125 , C12N9/0065 , C12N9/2462 , C12N15/8216 , C12N15/8222 , C12N15/8234 , C12N15/8257 , G01N33/57484 , G01N2333/4725 , G01N2500/02 , A23V2300/21
摘要： The invention is directed to food and food additive compositions comprising one or more human milk proteins produced in the seeds of a transgenic plant and methods of making the same. The invention is further directed to improved infant formula comprising such food supplement composition.
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公开(公告)号：US5888789A
公开(公告)日：1999-03-30
申请号：US460720
申请日：1995-06-02
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
CPC分类号： C12N15/8222 , C12N15/8234 , C12N15/8242 , C12N15/8257
摘要： This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins by malting of monocot plant seeds. The heterologous genes are expressed during germination of the seeds and isolated from a malt. Also disclosed are chimeric genes, vectors and methods relating to the present invention. Protein production by cell culture techniques is also described.
摘要翻译： 本发明提供了植物系统中异源蛋白质的分泌。 特别地，本发明提供了单子叶植物种子发芽的异源蛋白质的生产。 异种基因在种子发芽过程中表达，并从麦芽中分离出来。 还公开了与本发明相关的嵌合基因，载体和方法。 还描述了通过细胞培养技术的蛋白质生产。
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公开(公告)号：US5693506A
公开(公告)日：1997-12-02
申请号：US153563
申请日：1993-11-16
申请人： Raymond L. Rodriguez
发明人： Raymond L. Rodriguez
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/29 , C12N15/82 , C12P21/02 , A01H1/00 , A01H1/04 , C07H21/04
CPC分类号： C12N15/8222 , C12N15/8234 , C12N15/8242 , C12N15/8257
摘要： This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins in plant cultu1res and seeds. Where seeds are the source of the protein, the heterologous genes are expressed during germination and isolated from a malt.
摘要翻译： 本发明提供了植物系统中异源蛋白质的分泌。 特别地，本发明提供在植物培养物和种子中产生异源蛋白质。 当种子是蛋白质的来源时，异源基因在萌发期间表达并从麦芽中分离出来。
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