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    Sequences to facilitate incorporation of DNA into the genome of an organism
    1.
    发明授权
    Sequences to facilitate incorporation of DNA into the genome of an organism 有权

    公开(公告)号：US11981907B2

    公开(公告)日：2024-05-14

    申请号：US17152210

    申请日：2021-01-19

    申请人： AGBIOME, INC.

    发明人： Vadim Beilinson ,  James R. Henriksen

    IPC分类号： C12N15/82 ,  A01N57/20 ,  C12N9/10

    CPC分类号： C12N15/8277 ,  A01N57/20 ,  C12N9/1029 ,  C12N15/8209 ,  C12Y203/01183

    摘要： Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA N of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.

    SEQUENCES TO FACILITATE INCORPORATION OF DNA INTO THE GENOME OF AN ORGANISM
    2.
    发明申请
    SEQUENCES TO FACILITATE INCORPORATION OF DNA INTO THE GENOME OF AN ORGANISM 审中-公开

    公开(公告)号：US20170369898A1

    公开(公告)日：2017-12-28

    申请号：US15535750

    申请日：2015-12-18

    申请人： AgBiome, Inc.

    发明人： Vadim Beilinson ,  James R. Henriksen

    IPC分类号： C12N15/82 ,  C12N9/10

    CPC分类号： C12N15/8277 ,  A01N57/20 ,  C12N9/1029 ,  C12N15/8209 ,  C12Y203/01183

    摘要： Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.

    Plant selectable marker and plant transformation method
    6.
    发明授权
    Plant selectable marker and plant transformation method 有权
    植物选择标记和植物转化方法

    公开(公告)号：US06781044B2

    公开(公告)日：2004-08-24

    申请号：US09932328

    申请日：2001-08-17

    申请人： Raymond L. Rodriguez ,  Ning Huang

    发明人： Raymond L. Rodriguez ,  Ning Huang

    IPC分类号： C12N1582

    CPC分类号： C12N15/8201 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/2422 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8222 ,  C12N15/8234 ,  C12N15/8242 ,  C12N15/8257 ,  C12Y203/01183 ,  C12Y302/01001

    摘要： A plant transformation vector for transforming host plant cells with a chimeric selectable marker gene is disclosed. The gene includes, operatively linked in sequence in a 5′ to 3′ direction, (i) a DNA promoter sequence from the rice beta-glucanase 9 (gns9) gene; (ii) a selectable marker gene, and (iii) a 3′ untranslated terminator region. Also disclosed are a vector pair containing the transformation vector, a method of obtaining transformed monocots whose seeds produce a selected heterologous protein during sed germination, and a plant whose cells are transformed with the chimeric selectable marker gene.

    摘要翻译： 公开了用嵌合选择标记基因转化宿主植物细胞的植物转化载体。 该基因包括在5'至3'方向依次有效连接，（i）来自水稻β-葡聚糖酶9（gns9）基因的DNA启动子序列; （ii）选择标记基因，和（iii）3'非翻译终止子区。 还公开了含有转化载体的载体对，获得种子在萌发期间产生选择的异源蛋白质的转化单子叶植物的方法，以及用嵌合选择标记基因转化其细胞的植物。

    PLANT TRANSACTIVATION INTERACTION MOTIFS AND USES THEREOF
    10.
    发明申请
    PLANT TRANSACTIVATION INTERACTION MOTIFS AND USES THEREOF 审中-公开
    植物交互互动动画及其用途

    公开(公告)号：US20130205421A1

    公开(公告)日：2013-08-08

    申请号：US13757674

    申请日：2013-02-01

    申请人： Dow AgroSciences LLC

    发明人： JOSEPH PETOLINO ,  JIANQUAN LI ,  STEVEN L. EVANS ,  RYAN C. BLUE

    IPC分类号： C12N15/62

    CPC分类号： C12N15/625 ,  C07K14/415 ,  C07K14/4705 ,  C07K2319/71 ,  C07K2319/81 ,  C12N15/8216 ,  C12N15/8217 ,  C12Y203/01183

    摘要： This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

    摘要翻译： 本公开涉及用于增加感兴趣的多核苷酸的表达的组合物和方法。 一些实施方案涉及已在植物中鉴定的新型反式激活多肽及其变体及其使用方法。 具体实施方案涉及在融合蛋白中使用至少一种DNA结合多肽来将至少一种反式激活多肽或其变体靶向包含感兴趣多核苷酸的核酸上的特异性结合位点，使得其表达可能增加。