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公开(公告)号：US08575433B2
公开(公告)日：2013-11-05
申请号：US12508142
申请日：2009-07-23
申请人： David Cerf , Ruth Cong , Michael Freeman , Kevin E. McBride , Takashi Yamamoto
发明人： David Cerf , Ruth Cong , Michael Freeman , Kevin E. McBride , Takashi Yamamoto
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.
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公开(公告)号：US20090067700A1
公开(公告)日：2009-03-12
申请号：US11979881
申请日：2007-11-09
申请人： Peter Maton , Bruce Voorhees , Arghir Suciu , Steve W. Worrell , Sam D. Finkelstein , David S. Fryd , Kevin E. McBride
发明人： Peter Maton , Bruce Voorhees , Arghir Suciu , Steve W. Worrell , Sam D. Finkelstein , David S. Fryd , Kevin E. McBride
CPC分类号： G06T7/0012 , A61B6/461 , A61B6/465 , A61B6/5229 , G06T11/00 , G06T2200/24 , G06T2207/10116 , G06T2207/30061 , G16H40/63
摘要： A user may be provided with identified regions of interest superimposed on a larger image, which may permit the user to individually examine the regions of interest. The regions of interest may be independently windowed and/or leveled and/or magnified according to parameters associated with the region of interest, which parameters may be generated by a computer-aided detection algorithm.
摘要翻译： 可以向用户提供叠加在较大图像上的识别的感兴趣区域，这可以允许用户单独检查感兴趣的区域。 感兴趣区域可以根据与感兴趣区域相关联的参数独立地加窗和/或放大和/或放大，哪些参数可以由计算机辅助检测算法产生。
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公开(公告)号：US06943243B2
公开(公告)日：2005-09-13
申请号：US10360123
申请日：2003-02-06
申请人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
发明人： Tim N. Oulmassov , Kevin E. McBride , Paula C. Miller , John C. Anderson , Lyle D. Crossland , Thomas H. Adams , Barbara A. Qurollo , Victoria Gavrias
IPC分类号： A01H5/00 , A01K67/027 , A61K31/7088 , A61K48/00 , A61P31/00 , C07D307/33 , C07H21/04 , C12N1/19 , C12N5/04 , C12N5/10 , C12N9/18 , C12N15/09 , C12N15/81 , C12N15/82 , C12N15/85 , C12Q1/02 , C12Q1/68 , C12N5/14
CPC分类号： C07D307/33 , A61K48/00 , C12N15/81 , C12N15/8214 , C12N15/8216 , C12N15/8217 , C12N15/8238 , C12N15/85 , C12N2830/00 , C12N2830/001 , C12N2830/002 , C12N2830/15 , C12N2830/30 , C12N2830/36 , C12N2840/105 , C12N2840/20 , C12N2840/203
摘要： DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter (“lux box”) or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.
摘要翻译： 用于控制真核细胞和生物体中的基因表达的DNA构建体和其它组合物和方法源自细菌群体感测系统。 来自luxI启动子的一种或多种顺式元件（“lux框”）或功能上相似的序列并入真核启动子中。 来自转录调节物的LuxR家族的受体蛋白质在结合酰化的高丝氨酸内酯（AHL）化合物时，与lux框相互作用，调节启动子的活性。
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公开(公告)号：US20110294216A1
公开(公告)日：2011-12-01
申请号：US13120895
申请日：2009-10-22
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
摘要翻译： 提供了与使用磺酰脲类阻遏物相关的组合物和方法。 组合物包括特异性结合操作者的多肽，其中特异性结合受磺酰脲化合物调节。 组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞，以及真核生物，包括包含多核苷酸的植物和种子，和/或由该方法产生的。 还提供了向细胞或生物体提供磺酰脲反应性阻遏物并调节目标多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。
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公开(公告)号：US20110212838A1
公开(公告)日：2011-09-01
申请号：US13107212
申请日：2011-05-13
IPC分类号： A01N47/36 , C12N1/21 , C12N5/10 , C12N15/82 , C12N15/63 , C07H21/00 , C12N1/00 , A01P21/00 , A01P13/00
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
摘要翻译： 提供了与使用磺酰脲类阻遏物相关的组合物和方法。 组合物包括特异性结合操作者的多肽，其中特异性结合受磺酰脲化合物调节。 组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞，以及真核生物，包括包含多核苷酸的植物和种子，和/或由该方法产生的。 还提供了向细胞或生物体提供磺酰脲反应性阻遏物并调节目标多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。
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公开(公告)号：US06271444B1
公开(公告)日：2001-08-07
申请号：US09113690
申请日：1998-07-10
申请人： Kevin E. McBride , Jeffrey M. Staub
发明人： Kevin E. McBride , Jeffrey M. Staub
IPC分类号： C12N504
CPC分类号： C12N9/1092 , C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Provided are methods for increasing the production of protein in a plant cell by transforming plastids of plant cells with a construct comprising a promoter functional in a plant plastid, a ribosome binding site, DNA sequence of interest and a transcription termination region, and growing plant cells comprising the transformed plastids under conditions wherein the DNA encoding sequence is transcribed in the plastid. Also provided are methods for increasing protein production by fusing a coding sequence to a gene of interest to a secondary protein for cleavage or targetting of the protein of interest within the plastid, whereby high levels of expression of protein is achieved.
摘要翻译： 提供了通过用植物质体，核糖体结合位点，感兴趣的DNA序列和转录终止区功能的启动子和转录终止区的构建体转化植物细胞的质体来增加植物细胞中蛋白质的产生的方法，以及生长的植物细胞 在其中DNA编码序列在质体中转录的条件下包含转化的质体。 还提供了通过将编码序列融合到目的基因的二级蛋白质用于在质体内切割或靶向目标蛋白质来增加蛋白质产生的方法，由此实现了高水平的蛋白质表达。
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公开(公告)号：US06222097B1
公开(公告)日：2001-04-24
申请号：US08984320
申请日：1997-12-03
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
IPC分类号： A01H100
CPC分类号： C07K14/415 , C07K2319/00 , C07K2319/02 , C12N9/0071 , C12N15/8218 , C12N15/8222 , C12N15/8233 , C12N15/825
摘要： Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest in ovary tissue, particularly in very early fruit development. The DNA constructs comprise a transcriptional initiation regulatory region associated with gene expression in ovary tissue from immediately prior to anthesis through flower senescence.
摘要翻译： 提供了新的DNA构建体，其可以用作分子探针或插入到植物宿主中以提供对于在卵巢组织中感兴趣的DNA序列的转录的修饰，特别是在非常早期的果实发育中。 DNA构建体包含与通过花衰老开花之前的卵巢组织中与基因表达相关的转录起始调节区。
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公开(公告)号：US5866421A
公开(公告)日：1999-02-02
申请号：US593205
申请日：1996-01-29
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/8214 , C12N15/8216 , C12N15/8223
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.
摘要翻译： 用于植物细胞遗传工程的新型组合物和方法可用于提供蛋白质的植物或植物细胞的质体中增加的表达，该蛋白质产生当在不存在选择转化的方法的情况下生长植物或植物细胞时存在的表型 细胞。 苏云金芽孢杆菌细菌原毒素在植物叶绿体中的表达。
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公开(公告)号：US5545817A
公开(公告)日：1996-08-13
申请号：US209649
申请日：1994-03-11
申请人： Kevin E. McBride , David M. Stalker
发明人： Kevin E. McBride , David M. Stalker
CPC分类号： C12N15/8286 , C07K14/325 , C12N15/8214 , C12N15/8216 , C12N15/8223
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide increased expression in the plastids of a plant or plant cell of a protein which produces a phenotype which is present when the plant or plant cell is grown in the absence of means for selecting transformed cells. Expression of the Bacillus thuringiensis bacterial protoxin in a plant chloroplast is exemplified.
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公开(公告)号：US08993315B2
公开(公告)日：2015-03-31
申请号：US13120895
申请日：2009-10-22
IPC分类号： C12N15/00 , C07H21/04 , C07K14/245 , C07K14/195 , C12N15/63 , C12N15/82
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
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