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    Methods for the detection of hepatitis B and C viruses
    3.
    发明授权
    Methods for the detection of hepatitis B and C viruses 有权
    检测乙型和丙型肝炎病毒的方法

    公开(公告)号：US07776542B1

    公开(公告)日：2010-08-17

    申请号：US09269897

    申请日：1998-08-04

    Applicant: Katsumi Aoyagi ,  Chiharu Ohue ,  Kumiko Iida ,  Tatsuji Kimura ,  Shintaro Yagi

    Inventor： Katsumi Aoyagi ,  Chiharu Ohue ,  Kumiko Iida ,  Tatsuji Kimura ,  Shintaro Yagi

    IPC: G01N33/53 ,  G01N33/569 ,  G01N33/576

    CPC classification number: G01N33/5767 ,  G01N33/56983 ,  G01N2333/02 ,  G01N2333/18

    Abstract: A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.

    Abstract translation: 一种治疗含病毒样品的方法，其特征在于用含有（1）阴离子表面活性剂和（2）两性表面活性剂，非离子表面活性剂或蛋白质变性剂的处理溶液处理病毒样品; 使用所述处理方法的病毒测定方法; 一种用于处理含病毒样品的方法，其特征在于用含有（1）离液序列离子的处理溶液和（2）酸化剂处理含有病毒的样品; 使用所述处理方法的病毒测定方法; 一种病毒测定方法，其特征在于，在具有10个或更多个碳原子的烷基和二级，三级或季胺的表面活性剂存在下，基于它们与它们的探针的结合来测量病毒抗原和病毒抗体，或 非离子表面活性剂，或它们两者; 以及用于实施所述方法的单克隆抗体和产生其的杂交瘤。

    Method for Detection of Hepatitus B Virus
    4.
    发明申请
    Method for Detection of Hepatitus B Virus 审中-公开
    乙肝病毒检测方法

    公开(公告)号：US20090017443A1

    公开(公告)日：2009-01-15

    申请号：US11596663

    申请日：2005-05-19

    Applicant: Chiharu Ohue ,  Noboru Maki ,  Tatsuji Kimura

    Inventor： Chiharu Ohue ,  Noboru Maki ,  Tatsuji Kimura

    IPC: C12Q1/70

    CPC classification number: G01N33/5761

    Abstract: To provide a method for detection or quantification of hepatitis B virus (HBV) antigens in serum and a simple and highly user-friendly method for sample treatment for use in the detection or quantification thereof. The method for treatment of a sample containing hepatitis B virus (HBV) is characterized in that release of HBV antigens and disruption of antibodies that bind to HBV antigens are carried out by treating a sample containing HBV with a treatment agent containing (1) an acidifying agent and (2) a protein denaturant or an amphoteric surfactant or cationic surfactant having an alkyl group and a tertiary amine or a quaternary ammonium salt within a molecule.

    Abstract translation: 提供血清中乙型肝炎病毒（HBV）抗原的检测或定量的方法，以及用于检测或定量的用于样品处理的简单且高度用户友好的方法。 用于治疗含有乙型肝炎病毒（HBV）的样品的方法的特征在于通过用含有（1）酸化的处理剂处理含有HBV的样品来进行HBV抗原的释放和与HBV抗原结合的抗体的破坏 试剂和（2）分子内具有烷基和叔胺或季铵盐的蛋白质变性剂或两性表面活性剂或阳离子表面活性剂。

    METHODS FOR DETECTION OR MEASUREMENT OF VIRUSES
    6.
    发明申请
    METHODS FOR DETECTION OR MEASUREMENT OF VIRUSES 审中-公开
    检测或测量病毒的方法

    公开(公告)号：US20110262892A1

    公开(公告)日：2011-10-27

    申请号：US12349928

    申请日：2009-01-07

    Applicant: Katsumi Aoyagi ,  Chiharu Ohue ,  Kumiko Iida ,  Tatsuji Kimura ,  Shintaro Yagi

    Inventor： Katsumi Aoyagi ,  Chiharu Ohue ,  Kumiko Iida ,  Tatsuji Kimura ,  Shintaro Yagi

    IPC: C12Q1/70 ,  C07K16/08

    CPC classification number: G01N33/5767 ,  G01N33/56983 ,  G01N2333/02 ,  G01N2333/18

    Abstract: A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.

    Abstract translation: 一种治疗含病毒样品的方法，其特征在于用含有（1）阴离子表面活性剂和（2）两性表面活性剂，非离子表面活性剂或蛋白质变性剂的处理溶液处理含病毒样品; 使用所述处理方法的病毒测定方法; 一种用于处理含病毒样品的方法，其特征在于用含有（1）离液序列离子的处理溶液和（2）酸化剂处理含有病毒的样品; 使用所述处理方法的病毒测定方法; 一种病毒测定方法，其特征在于，在具有10个或更多个碳原子的烷基和二级，三级或季胺的表面活性剂存在下，基于它们与其探针的结合度来测量病毒抗原和病毒抗体，或 非离子表面活性剂，或它们两者; 以及用于实施所述方法的单克隆抗体和产生其的杂交瘤。

    METHOD FOR DETECTION OF HEPATITIS B VIRUS
    7.
    发明申请
    METHOD FOR DETECTION OF HEPATITIS B VIRUS 审中-公开
    检测乙型肝炎病毒的方法

    公开(公告)号：US20100291546A1

    公开(公告)日：2010-11-18

    申请号：US12828209

    申请日：2010-06-30

    Applicant: Chiharu Ohue ,  Noboru Maki ,  Tatsuji Kimura

    Inventor： Chiharu Ohue ,  Noboru Maki ,  Tatsuji Kimura

    IPC: C12Q1/70 ,  C12N7/00

    CPC classification number: G01N33/5761

    Abstract: To provide a method for detection or quantification of hepatitis B virus (HBV) antigens in serum and a simple and highly user-friendly method for sample treatment for use in the detection or quantification thereof. The method for treatment of a sample containing hepatitis B virus (HBV) is characterized in that release of HBV antigens and disruption of antibodies that bind to HBV antigens are carried out by treating a sample containing HBV with a treatment agent containing (1) an acidifying agent and (2) a protein denaturant or an amphoteric surfactant or cationic surfactant having an alkyl group and a tertiary amine or a quaternary ammonium salt within a molecule.

    Abstract translation: 提供血清中乙型肝炎病毒（HBV）抗原的检测或定量的方法，以及用于检测或定量的用于样品处理的简单且高度用户友好的方法。 用于治疗含有乙型肝炎病毒（HBV）的样品的方法的特征在于通过用含有（1）酸化的处理剂处理含有HBV的样品来进行HBV抗原的释放和与HBV抗原结合的抗体的破坏 试剂和（2）分子内具有烷基和叔胺或季铵盐的蛋白质变性剂或两性表面活性剂或阳离子表面活性剂。

    HCV GENE
    8.
    发明申请
    HCV GENE 审中-公开
    HCV基因

    公开(公告)号：US20100173298A1

    公开(公告)日：2010-07-08

    申请号：US12597692

    申请日：2008-04-28

    Applicant: Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai ,  Chiharu Ohue

    Inventor： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai ,  Chiharu Ohue

    IPC: C12Q1/68 ,  C07H21/04 ,  C07K14/005 ,  C07H21/02 ,  C12N15/63 ,  C12N5/071 ,  C12N7/00

    CPC classification number: G01N33/5008 ,  C07K14/005 ,  C12N15/86 ,  C12N2770/24222 ,  C12N2770/24243 ,  C12Q1/707 ,  G01N33/5767 ,  G01N2333/186 ,  G01N2500/00

    Abstract: The present invention provides a hepatitis C virus gene, a replicon RNA derived from the gene, a replicon-replicating cell into which the replicon RNA is introduced, and a method for screening a drug using the replicon-replicating cell. By introducing a replicon RNA comprising (A) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 5; (B) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 7; (C) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 6; (D) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 8; or (E) a polynucleotide having a nucleic acid sequence having a homology of not less than 90% with the nucleic acid sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7; or a replicon RNA of the genotype 1b comprising nucleotides coding for the 1804th amino acid leucine and the 1966th amino acid lysine in the amino acid sequence of a hepatitis C virus polyprotein and a polynucleotide coding for an NS4B protein, into a cell, the replicon-replicating cell can be prepared. By using the replicon-replicating cell, the screening for the drug can be carried out.

    Abstract translation: 本发明提供丙型肝炎病毒基因，来源于该基因的复制子RNA，引入复制子RNA的复制子复制细胞和使用复制子复制细胞筛选药物的方法。 通过引入包含（A）具有SEQ ID NO：5所示核酸序列的多核苷酸的复制子RNA; （B）具有SEQ ID NO：7所示核酸序列的多核苷酸; （C）编码具有SEQ ID NO：6所示氨基酸序列的多肽的多核苷酸; （D）编码具有SEQ ID NO：8所示氨基酸序列的多肽的多核苷酸; 或（E）具有与SEQ ID NO：5或SEQ ID NO：7所示核酸序列同源性不低于90％的核酸序列的多核苷酸; 或基因型1b的复制子RNA，其包含编码丙型肝炎病毒多蛋白的氨基酸序列中的第1804位氨基酸亮氨酸和第1966位氨基酸赖氨酸的核苷酸和编码NS4B蛋白的多核苷酸的复制子RNA， 可以制备复制细胞。 通过使用复制子复制细胞，可以进行药物的筛选。

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