知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

298 条记录 (耗时 0.033 秒)

    Antibodies and method for determining deletions in HBV pre-S2 region
    5.
    发明授权
    Antibodies and method for determining deletions in HBV pre-S2 region 有权

    公开(公告)号：US09714284B2

    公开(公告)日：2017-07-25

    申请号：US14333346

    申请日：2014-07-16

    申请人： NATIONAL HEALTH RESEARCH INSTITUTES

    发明人： Wenya Huang ,  Ih-Jen Su ,  Yun-Ping Lee

    IPC分类号： C12N7/00 ,  C07K16/08 ,  G01N33/574 ,  G01N33/576

    CPC分类号： C07K16/082 ,  C07K2317/34 ,  G01N33/57438 ,  G01N33/5764 ,  G01N2800/085 ,  G01N2800/50 ,  G01N2800/54

    摘要： A HBS-specific antibody, a LHBS-specific antibody, a WT LHBS-specific antibody, an immunoassay kit comprising the antibodies, and a method of detecting pre-S2 deletion mutant LHBS using the immunoassay kit are disclosed herein. The method comprises incubating a biological sample with a first antibody to captured HBS proteins; detecting the LHBS and WT LHBS bound to the immobilized first antibody, respectively; and calculating the amount of the pre-S2 deletion mutant LHBS protein by subtracting the amount of the WT LHBS protein from that of the LHBS protein. Advantageously, by the method described herein, the amount of the pre-S2 deletion mutant LHBS, a potential high-risk marker for HCC incidence in chronic HBV carriers and recurrence in HCC patients after hepatectomy surgery, in a biological sample may be easily calculated without mutual influence between the WT and pre-S mutant LHBS while reducing the labor-intensive process for cloning each gene product before analysis.