知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

1918 条记录 (耗时 0.032 秒)

    mRNA display antibody library and methods
    74.
    发明申请
    mRNA display antibody library and methods 有权

    公开(公告)号：US20210261948A1

    公开(公告)日：2021-08-26

    申请号：US17316381

    申请日：2021-05-10

    申请人： NantBio, Inc.

    发明人： Clifford Anders Olson ,  Kayvan Niazi

    IPC分类号： C12N15/10 ,  C40B40/10 ,  C12N15/86 ,  C12N5/09 ,  C40B40/02 ,  C40B50/06

    摘要： Compositions, methods and uses of a recombinant virus and/or recombinant viral vector encoding a distinct antibody or antibody fragment generated from high-diversity nucleic acid library are presented. Preferably, the recombinant virus is genetically modified, low immunogenic virus, for example, an E2b-deleted adenovirus. The high-diversity nucleic acid library comprises or is derived from (1) a VH-CDR1/2 sub-library, (2) a plurality of VH-CDR3 sub-libraries, and (3) a VL sub-library, each of which comprises a plurality of members. Preferably, each member of the sub-libraries comprises at least one random cassette that has a plurality of degenerate base positions. In an especially preferred embodiment, at least portions of at least two members of the VH-CDR1/2 sub-library, the plurality of VH-CDR3 sub-libraries, and the VL sub-library are recombined to form an expression library member in an expression library, where each member of the expression library encodes a distinct antibody or antibody fragment.

    FUCOSE-BINDING PROTEIN, METHOD FOR PRODUCING SAME, AND USE OF SAME
    75.
    发明申请
    FUCOSE-BINDING PROTEIN, METHOD FOR PRODUCING SAME, AND USE OF SAME 有权

    公开(公告)号：US20210261629A1

    公开(公告)日：2021-08-26

    申请号：US17267891

    申请日：2019-08-08

    申请人： TOSOH CORPORATION ,  SAGAMI CHEMICAL RESEARCH INSTITUTE

    发明人： Masahiro HAYASHI ,  Takahiro MARUYAMA ,  Hiroyuki ITO ,  Aya ASAGOSHI ,  Kouta HATAYAMA ,  Megumi HOYA

    IPC分类号： C07K14/195 ,  B01J20/24 ,  B01J20/30 ,  C12N5/09 ,  C07H1/06 ,  B01D15/38

    摘要： The present invention aims to provide a fucose-binding protein that shows improved productivity in cases of expression in a host such as Escherichia coli, improved binding affinity to a fucose-containing sugar chain such as a sugar chain containing a structure composed of Fucα1-2Galβ1-3GlcNAc and/or Fucα1-2Galβ1-3GalNAc, and/or improved thermal stability. The above object is achieved by deleting a plurality of amino acid residues in the C-terminal side of the amino acid sequence of the fucose-binding protein BC2LCN of SEQ ID NO: 1, and, when necessary, substituting the glycine residue at position 36 in SEQ ID NO: 1 with a cysteine residue, substituting the glutamine residue at position 39 in SEQ ID NO: 1 with a leucine residue or methionine residue, substituting the glutamine residue at position 65 in SEQ ID NO: 1 with a leucine residue, substituting the cysteine residue at position 72 in SEQ ID NO: 1 with a glycine residue or alanine residue, substituting the glutamic acid residue at position 81 in SEQ ID NO: 1 with a cysteine residue, glutamine residue, histidine residue, or methionine residue, and/or substituting the glycine residue identified as the residue at position 36 in SEQ ID NO: 1 with a cysteine residue.