公开(公告)号：US09766206B2

公开(公告)日：2017-09-19

申请号：US14039995

申请日：2013-09-27

申请人： ProteinSimple

发明人： Tom W. Yang ,  David John Roach

IPC分类号： G01N27/447 ,  C07K1/28 ,  C07K1/26

CPC分类号： G01N27/44782 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44791

摘要： An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

公开(公告)号：US20170160230A1

公开(公告)日：2017-06-08

申请号：US15430312

申请日：2017-02-10

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Thomas JACKSON

IPC分类号： G01N27/447 ,  F16B2/18 ,  B01L3/00 ,  C07K1/26 ,  C07H1/06

CPC分类号： G01N27/44739 ,  B01L3/502 ,  B01L3/563 ,  B01L3/565 ,  B01L9/52 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/04 ,  B01L2200/0689 ,  B01L2300/041 ,  B01L2300/06 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2400/0421 ,  B33Y80/00 ,  C07H1/06 ,  C07K1/26 ,  F16B2/18 ,  G01N27/44704 ,  Y10T29/49117 ,  Y10T403/7009

摘要： An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. In one example embodiment, the expanding earn lock includes a base plate with a first surface adapted to engage the first buffer core assembly and a follower plate having second surface adapted to engage the second buffer core assembly, buffer dam or buffer displacement dam. The base plate and the follower plate are slidably coupled together and are designed for insertion between the first buffer core assembly and the second buffer core assembly, buffer dam or buffer displacement dam in the electrophoresis container. A cam is positioned between and moveably coupled with the base plate and the follower plate. The cam is movable from a first position to a second position to urge the first and second surfaces to secure the gel cassette to the first and second buffer core assemblies. Also provided herein is a buffer displacement dam. Also provided herein are kits and assemblies which incorporate the expanding cam and buffer displacement dam described herein.

公开(公告)号：US09605307B2

公开(公告)日：2017-03-28

申请号：US12658591

申请日：2010-02-08

申请人： Roger Chen ,  Randy Davis

发明人： Roger Chen ,  Randy Davis

IPC分类号： B01D59/02 ,  B01D59/42 ,  B01D59/50 ,  B01D61/42 ,  B01D61/58 ,  C02F1/469 ,  C07K1/26 ,  C08F2/58 ,  C25B7/00 ,  C25B15/00 ,  G01F1/64 ,  G01L1/20 ,  G01L9/18 ,  C12Q1/68 ,  B82Y5/00 ,  G01N33/487

CPC分类号： B29C67/20 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q2565/631

摘要： Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US09409942B2

公开(公告)日：2016-08-09

申请号：US13951285

申请日：2013-07-25

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Deb Chatterjee ,  Mary Longo ,  Elizabeth Flynn ,  Robert Oberfelder

IPC分类号： C07K9/00 ,  C07K1/26 ,  C07K1/107 ,  C07K1/13 ,  C12N9/02 ,  C12P21/02 ,  C07K14/00

CPC分类号： C07K1/26 ,  C07K1/1077 ,  C07K1/13 ,  C07K14/001 ,  C07K2319/00 ,  C07K2319/01 ,  C12N9/0036 ,  C12P21/02 ,  Y02P20/52

摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies thereby facilitating rapid isolation and purification of recombinant proteins. The invention further provides methods for producing protein molecular weight ladders for us in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

摘要翻译： 本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。 本发明的方法通过形成包含编码与包含配对蛋白的多肽有效连接的多肽的遗传序列的基因构建体进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体，从而促进快速分离和纯化 重组蛋白。 本发明还提供了在蛋白质凝胶电泳中为我们生产蛋白质分子量梯子的方法，以及通过这些方法制备的蛋白质和蛋白质分子量梯。

公开(公告)号：US09377438B2

公开(公告)日：2016-06-28

申请号：US14446379

申请日：2014-07-30

申请人： The Johns Hopkins University

发明人： Charles C. Young ,  Alex J. Proescher ,  Emily E. Smith

IPC分类号： G01N27/447 ,  C07K1/26 ,  G01N33/559 ,  C12M1/33 ,  G01N30/88

CPC分类号： G01N27/44717 ,  C07K1/26 ,  G01N27/44721 ,  G01N33/559 ,  G01N2030/8827 ,  Y10S435/973

摘要： A kit for separating and concentrating nucleic acid and protein targets includes labeled reagents which affect simultaneous co-purification and concentration of a nucleic acid and a protein, a gel isotachophoresis separation unit to which a sample comprising the nucleic acid and the protein is added, a detection unit for the detection of the presence of the nucleic acid and the protein, and instructions for use. The gel electrophoresis includes a gel box having a negative electrode side and a positive electrode side, the negative electrode side being filled with a first buffer comprising 2-Hydroxy-N-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid buffer and the positive electrode side being filled with a second buffer being different than the first buffer. The gel isotachophoresis separation is configured to subject the sample to isotachophoresis using a voltage.

摘要翻译： 用于分离和浓缩核酸和蛋白质靶标的试剂盒包括影响核酸和蛋白质的同时共同纯化和浓缩的标记试剂，添加了包含核酸和蛋白质的样品的凝胶等电泳分离单元， 用于检测核酸和蛋白质的存在的检测单元，以及使用说明书。 凝胶电泳包括具有负极侧和正极侧的凝胶盒，负极侧填充有包含2-羟基-N-（三（羟甲基）甲基）-3-氨基丙磺酸缓冲液的第一缓冲液， 正极侧被填充有与第一缓冲器不同的第二缓冲器。 凝胶等速电泳分离被配置为使样品使用电压进行等速电泳。

公开(公告)号：US09063082B2

公开(公告)日：2015-06-23

申请号：US13512354

申请日：2010-11-26

申请人： Erik Bjerneld ,  Jan-Olof Johansson ,  Ronnie Palmgren ,  Elsemarie Bjellqvist

发明人： Bengt Bjellqvist ,  Erik Bjerneld ,  Jan-Olof Johansson ,  Ronnie Palmgren

IPC分类号： C08F2/46 ,  G01N27/447 ,  C07K1/26

CPC分类号： G01N27/44747 ,  C07K1/26 ,  C08F2/46

摘要： The invention relates to a method to produce gels for electrophoresis, wherein at least two monomer solutions are printed in a pattern on a substrate and the printed substrate is exposed to electromagnetic or ionising radiation so as to initiate polymerisation. The gels are useful for electrophoretic separation of proteins, peptides and/or nucleic acids.

摘要翻译： 本发明涉及一种生产电泳用凝胶的方法，其中至少两种单体溶液以衬底上的图案印刷，并将印刷的基材暴露于电磁辐射或电离辐射以引发聚合。 凝胶可用于蛋白质，肽和/或核酸的电泳分离。

公开(公告)号：US09017536B2

公开(公告)日：2015-04-28

申请号：US12448279

申请日：2007-12-26

申请人： Kazuyuki Oishi ,  Izumi Omoto ,  Eriko Kusaka ,  Toshiki Kawabe

发明人： Kazuyuki Oishi ,  Izumi Omoto ,  Eriko Kusaka ,  Toshiki Kawabe

IPC分类号： B01D57/02 ,  G01N27/447 ,  C07K1/26

CPC分类号： G01N27/44747 ,  C07K1/26 ,  G01N2333/805

摘要： An object of the present invention is to provide a method for measuring hemoglobin that enables short-time, high-accuracy measurement of hemoglobin, in particular stable hemoglobin A1c, which is used as a diagnostic indicator of diabetes mellitus, and an electrophoresis apparatus that is suitably used in this measurement method. The present invention provides a method for measuring hemoglobins using electrophoresis, which includes: using a migration path having an inner surface coated with a cationic substance to be immobilized on the inner surface or a migration path having an inner surface made of a cationic material; and using a buffer solution containing a water-soluble polymer having an anionic group as an electrophoresis buffer solution.

摘要翻译： 本发明的目的在于提供一种测定血红蛋白的方法，其能够进行用作糖尿病诊断指标的血红蛋白，特别是稳定血红蛋白A1c的短时间，高精度测定，以及电泳装置 适用于该测量方法。 本发明提供一种使用电泳测定血红蛋白的方法，其包括：使用具有被固定在内表面的阳离子性物质的内表面的迁移路径或具有由阳离子性物质构成的内表面的迁移路径; 并使用含有具有阴离子基团的水溶性聚合物的缓冲溶液作为电泳缓冲溶液。

公开(公告)号：US08926813B2

公开(公告)日：2015-01-06

申请号：US13567595

申请日：2012-08-06

申请人： John S. Oliver

发明人： John S. Oliver

IPC分类号： C07K1/26 ,  G01N33/487 ,  G01N27/327

CPC分类号： G01N33/48721 ,  G01N27/3278 ,  G01N27/4473 ,  Y10S977/957

摘要： Devices and methods for detecting the length of analytes, and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals.

摘要翻译： 提供了用于检测分析物的长度和/或测序分析物的装置和方法，其中当分析物穿过流体通道时获得两个或多个电信号。 检测与生物聚合物杂交的探针和/或分析物（例如，生物聚合物）的长度的相对位置不依赖于给定电信号的检测事件之间的绝对时间来确定与生物聚合物相关联的距离。 相反，多个信号作为时间的函数获得），其对应于沿着生物聚合物通过的流体通道的已知位置处的多个检测器体积，并且距离由多个信号确定。

公开(公告)号：US08747637B2

公开(公告)日：2014-06-10

申请号：US12170287

申请日：2008-07-09

申请人： Shantanu Bhattacharya ,  Shubhra Gangopadhyay ,  Keshab Gangopedhyay ,  Nripen Chanda ,  Paul Sharp

发明人： Shantanu Bhattacharya ,  Shubhra Gangopadhyay ,  Keshab Gangopedhyay ,  Nripen Chanda ,  Paul Sharp

IPC分类号： C07K1/26 ,  B01D57/02 ,  H01B1/22 ,  G01N27/447

CPC分类号： G01N27/44747 ,  G01N27/44704

摘要： The invention relates to compositions and methods of using electrophoresis separation matrices. The invention provides nano-particle comprising separation matrices having increased conductivity at low voltage.

摘要翻译： 本发明涉及使用电泳分离基质的组合物和方法。 本发明提供包含在低电压下具有增加的电导率的分离基质的纳米颗粒。

公开(公告)号：US20130153419A1

公开(公告)日：2013-06-20

申请号：US13331946

申请日：2011-12-20

申请人： LEI LI ,  DENNIS YEE

发明人： LEI LI ,  DENNIS YEE

IPC分类号： C12P21/00 ,  C07K1/26 ,  C09K3/00 ,  C07K2/00 ,  C07K14/00

CPC分类号： C12P21/02

摘要： The invention provides methods of generating physical analog polypeptides, and polypeptides generated employing the methods of the invention, that have the same amino acid composition of a reference protein, but have an unrelated primary sequence. The invention further provides compositions and methods employing such proteins.

摘要翻译： 本发明提供产生物理类似物多肽的方法，以及使用本发明方法产生的具有参考蛋白的相同氨基酸组成但具有不相关的一级序列的多肽。 本发明还提供了使用这种蛋白质的组合物和方法。