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    Quantitative analysis of uric acid
    61.
    发明授权
    Quantitative analysis of uric acid 失效
    尿酸定量分析

    公开(公告)号：US4379840A

    公开(公告)日：1983-04-12

    申请号：US278677

    申请日：1981-06-29

    Applicant: Gunther Gorka ,  Klaus Stinshoff

    Inventor： Gunther Gorka ,  Klaus Stinshoff

    IPC: G01N33/50 ,  C12Q1/62 ,  C12N9/04 ,  C12N9/96 ,  C12Q1/30 ,  C12Q1/32

    CPC classification number: C12Q1/62 ,  Y10S435/81

    Abstract: This invention relates to a process for determining the uric acid content of biological material by contacting the biological material with a reagent composition comprised of uricase, catalase, aldehyde dehydrogenase, a lower alkanol, and nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotied phosphate, the improvement which comprises determining the uric acid content in the presence of 2-mercaptosuccinic acid.

    Abstract translation: 本发明涉及通过使生物材料与由尿酸酶，过氧化氢酶，醛脱氢酶，低级链烷醇和烟酰胺腺嘌呤二核苷酸或烟酰胺腺嘌呤二核苷酸磷酸酯组成的试剂组合物接触来测定生物材料的尿酸含量的方法， 包括在2-巯基琥珀酸存在下测定尿酸含量。

    Method and reagent for the quantitative determination of hydrogen peroxide and precursors thereof
    62.
    发明授权
    Method and reagent for the quantitative determination of hydrogen peroxide and precursors thereof 失效
    用于定量测定过氧化氢及其前体的方法和试剂

    公开(公告)号：US4260679A

    公开(公告)日：1981-04-07

    申请号：US62742

    申请日：1979-08-01

    Applicant: Mitsuru Tsuda ,  Toshio Tatano

    Inventor： Mitsuru Tsuda ,  Toshio Tatano

    IPC: G01N33/50 ,  C12Q1/28 ,  G01N31/22 ,  C12Q1/54 ,  C12Q1/60 ,  C12Q1/62

    CPC classification number: C12Q1/28 ,  G01N31/228 ,  C12Q2326/00 ,  C12Q2326/96 ,  Y10T436/144444 ,  Y10T436/206664

    Abstract: A method of and reagent for determining hydrogen peroxide, in which the hydrogen peroxide containing sample is treated with a colouring reagent comprising a peroxidase, a hydrogen donor and an electron or radical acceptor which react stoichiometrically with the hydrogen peroxide to produce a pigment which is then measured colorimetrically. In accordance with the invention the hydrogen donor is specifically a compound of the formula ##STR1## where R.sub.1 is CH.sub.3, C.sub.2 H.sub.5, CH.sub.2 OH, CH.sub.2 CH.sub.2 OH, CH.sub.2 CH.sub.2 NH.sub.2 or COCH.sub.3 ;R.sub.2 is CH.sub.2 OH, CH.sub.2 CH.sub.2 OH, CH.sub.2 CH.sub.2 NH.sub.2, CH.sub.2 NHCOCH.sub.3, CH.sub.2 CH.sub.2 NHCOCH.sub.3 or COCH.sub.3 ;R.sub.3 is H, CH.sub.3, C.sub.2 H.sub.5 or OCH.sub.3 ; andR.sub.4 is H or CH.sub.3.The method and reagent may also be used in the determination of other materials such as cholesterol, phospholipid, glucose and uric acid, by initial treatment with an oxidase thereby quantitatively to produce hydrogen peroxide which is then determined colorimetrically by the method of the invention.

    Abstract translation: 用于测定过氧化氢的方法和试剂，其中含过氧化氢的样品用包含过氧化物酶，氢供体和电子或自由基受体的着色剂处理，所述受体与化学计量与过氧化氢反应以产生颜料， 比色测定 根据本发明，氢供体特别是式IMA的化合物，其中R 1是CH 3，C 2 H 5，CH 2 OH，CH 2 CH 2 OH，CH 2 CH 2 NH 2或COCH 3; R2是CH2OH，CH2CH2OH，CH2CH2NH2，CH2NHCOCH3，CH2CH2NHCOCH3或COCH3; R3是H，CH3，C2H5或OCH3; R4是H或CH3。 方法和试剂也可以用于通过用氧化酶初始处理来测定其它物质如胆固醇，磷脂，葡萄糖和尿酸，从而定量地产生过氧化氢，然后用本发明的方法用比色法测定。

    Uric acid determination
    63.
    发明授权
    Uric acid determination 失效
    尿酸测定

    公开(公告)号：US4072627A

    公开(公告)日：1978-02-07

    申请号：US710801

    申请日：1976-08-02

    Applicant: E. Melvin Gindler

    Inventor： E. Melvin Gindler

    IPC: C12Q1/62 ,  G01N33/52 ,  C09K3/00 ,  G01N31/06 ,  G01N31/22 ,  G01N33/16

    CPC classification number: G01N33/52 ,  C12Q1/62 ,  Y10T436/148888

    Abstract: An aqueous solution useful in connection with a redox type spectrophotometric or colorimetric determination of uric acid in a biologic fluid is disclosed. The solution contains either a multivalent metallic ion reducible to a lower valence state by uric acid, a water soluble chelating compound capable of complexing with a metallic ion after reduction by uric acid to yield, in complexed form, a colored complex, or a combination of said ion and said chelating compound. The solution also contains, as an added constituent, imidazole, an alpha-amino acid, or a combination thereof. This constituent is present in an amount such that, when said solution contains a biologic fluid containing protein and uric acid, a buffer system such that the pH of the solution is 6 to 12, and a multivalent metal ion, the protein in the fluid does not significantly reduce said multivalent ions present in the solution.

    Abstract translation: 公开了与生物液中的尿酸的氧化还原型分光光度法或比色测定法有关的水溶液。 该溶液含有可以通过尿酸还原成低价态的多价金属离子，一种水溶性螯合化合物，其在与尿酸还原后能够与金属离子络合，以复合形式产生着色复合物，或组合 所述离子和所述螯合化合物。 该溶液还含有作为添加成分的咪唑，α-氨基酸或其组合。 该成分的存在量使得当所述溶液含有含有蛋白质和尿酸的生物液时，使得溶液的pH为6至12的缓冲系统和多价金属离子，流体中的蛋白质 不显着降低溶液中存在的所述多价离子。

    Reagent formulation for uric acid assay
    66.
    发明授权
    Reagent formulation for uric acid assay 失效
    尿酸测定试剂配方

    公开(公告)号：US3928137A

    公开(公告)日：1975-12-23

    申请号：US46743174

    申请日：1974-05-06

    Applicant: MALLINCKRODT INC

    Inventor： MONTE ALEXANDER A ,  CHIANG CHING

    IPC: C12Q1/62 ,  G01N33/72 ,  G01N31/14 ,  G01N33/16

    CPC classification number: G01N33/721 ,  C12Q1/62

    Abstract: An enzyme reagent formulation for use in assaying a biological specimen for uric acid is prepared as a granular, water-soluble, anhydrous, storage-stable mixture containing uricase, potassium chloride, mannitol, gum acacia, bovine serum albumin, glycine, sodium carbonate and a nitrogen containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst. The surfactant contains polyoxypropylene chains having an average molecular weight of between about 750 and about 6750, and polyoxyethylene chains constituting between about 10 and about 80 weight percent of the surfactant. The surfactant has an advantageous effect upon granulation, dissolution and storage stability of the reagent formulation.

    Abstract translation: 制备用于测定尿酸生物样本的酶试剂制剂，其为含有尿酸酶，氯化钾，甘露糖醇，阿拉伯胶，牛血清白蛋白，甘氨酸，碳酸钠的颗粒状，水溶性，无水，储存稳定的混合物。 通过在催化剂存在下通过乙二胺与环氧丙烷和环氧乙烷的顺序反应获得的含氮聚氧化烯非离子表面活性剂。 表面活性剂含有平均分子量在约750至约6750之间的聚氧丙烯链，构成表面活性剂约10至约80重量％的聚氧乙烯链。 表面活性剂对试剂制剂的造粒，溶解和储存稳定性具有有利的作用。

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