公开(公告)号：US07078041B2

公开(公告)日：2006-07-18

申请号：US10623429

申请日：2003-07-18

申请人： David M. Koelle ,  Zhi Liu ,  Lawrence Corey

发明人： David M. Koelle ,  Zhi Liu ,  Lawrence Corey

IPC分类号： A61K39/245

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5158 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/16622 ,  C12N2710/16634 ,  C12N2710/24143

摘要： Described is a method of identifying an immunologically active antigen of a virus that attacks skin, as well as a method of enriching a population of lymphocytes for T lymphocytes that are specific to a virus that attacks skin. Also provided are HSV antigens and epitopes that are useful for the prevention and treatment of HSV infection that have been identified via the methods of the invention. T-cells having specificity for antigens of the invention have demonstrated cytotoxic activity against cells loaded with virally-encoded peptide epitopes, and in many cases, against cells infected with HSV. The identification of immunogenic antigens responsible for T-cell specificity provides improved anti-viral therapeutic and prophylactic strategies. Compositions containing antigens or polynucleotides encoding antigens of the invention provide effectively targeted vaccines for prevention and treatment of HSV infection.

公开(公告)号：US07078029B2

公开(公告)日：2006-07-18

申请号：US10427739

申请日：2003-05-01

申请人： Neal A. DeLuca

发明人： Neal A. DeLuca

IPC分类号： A61K48/00 ,  C12N15/00 ,  C12N15/64

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/16622 ,  C12N2710/16643

摘要： The present invention provides an HSV having a genome with a mutation of a TAATGARAT sequence such that, in the presence of a ICP4 gene product, a native immediate early gene is expressed from the genome with delayed kinetics, the genome having a further inactivating mutation of each of the genes encoding ICP4.

公开(公告)号：US20060116340A1

公开(公告)日：2006-06-01

申请号：US10808042

申请日：2004-03-24

申请人： Alfred Lewin ,  David Bloom ,  Gregory Schultz ,  Sonal Tuli ,  Jia Liu

发明人： Alfred Lewin ,  David Bloom ,  Gregory Schultz ,  Sonal Tuli ,  Jia Liu

IPC分类号： A61K48/00 ,  C12N5/06 ,  C07H21/02

CPC分类号： C07K14/005 ,  C12N2510/02 ,  C12N2710/16622

摘要： Hammerhead ribozymes that target components critical to HSV replication (ICP4, UL20, UL30, and UL54) were synthesized and shown to efficiently cleave target RNA encoding a portion of these components in in vitro assays. Several cloned cell lines stably expressing these ribozymes were established. HSV-mediated plaque formation was dramatically reduced in cells stably transfected with an anti-HSV ribozyme compared to non-transfected cells.

摘要翻译： 合成针对HSV复制（ICP4，UL20，UL30和UL54）关键组分的锤头核酶，并显示出在体外测定中有效切割编码这些成分的一部分的靶RNA。 建立了稳定表达这些核酶的几种克隆细胞系。 与未转染细胞相比，用抗HSV核酶稳定转染的细胞中HSV介导的斑块形成显着降低。

公开(公告)号：US20060051368A1

公开(公告)日：2006-03-09

申请号：US11187652

申请日：2005-07-22

申请人： Patricia Spear ,  Sharmila Manoj ,  Cheryl Jogger ,  Dawn Myscofski ,  Miri Yoon

发明人： Patricia Spear ,  Sharmila Manoj ,  Cheryl Jogger ,  Dawn Myscofski ,  Miri Yoon

IPC分类号： A61K39/12 ,  C07H21/04 ,  C07K14/035 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K2039/525 ,  C12N2710/16622

摘要： The present invention relates to compositions and methods related to mutant herpes simplex virus nucleic acid and proteins that find use in analyzing, diagnosing, and regulating viral infection.

摘要翻译： 本发明涉及可用于分析，诊断和调节病毒感染的突变单纯疱疹病毒核酸和蛋白质相关的组合物和方法。

公开(公告)号：US20050271620A1

公开(公告)日：2005-12-08

申请号：US10504460

申请日：2003-02-12

申请人： Susanne Brown ,  Joe Conner

发明人： Susanne Brown ,  Joe Conner

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K47/48 ,  A61K48/00 ,  A61P35/00 ,  C07K14/005 ,  C07K14/82 ,  C07K16/00 ,  C07K19/00 ,  C12N7/00 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K48/00 ,  C12N2710/16622

摘要： There is provided an HSV complex which comprises an avirulent HSV and a targeting agent which allows the HSV particle to infect and lyse a specific targeted cell. The inventors have found a way in which avirulent HSV can be targeted to disease cells, e.g. cancer cells, by incorporating an antibody binding domain into one or more viral glycoproteins.

摘要翻译： 提供了HSV复合物，其包含无毒性HSV和靶向剂，其允许HSV颗粒感染和裂解特异性靶向细胞。 本发明人已经找到了无毒性HSV可以靶向疾病细胞的方法，例如， 通过将抗体结合结构域掺入到一种或多种病毒糖蛋白中来形成癌细胞。

公开(公告)号：US20050208657A1

公开(公告)日：2005-09-22

申请号：US10865538

申请日：2004-06-09

申请人： Brian Dalby ,  Robert Bennett

发明人： Brian Dalby ,  Robert Bennett

IPC分类号： A61K48/00 ,  C07K14/035 ,  C12N15/85 ,  C12N15/87 ,  C12N15/74

CPC分类号： C07K14/005 ,  A61K48/0025 ,  C07K2319/00 ,  C12N15/85 ,  C12N15/87 ,  C12N2710/16622 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2840/20

摘要： The invention provides methods for modulating a cellular process by contacting a cell in culture with a cell process-modifying molecule attached to a translocating polypeptide. For example, in one embodiment, a cell in culture is transfected with a target gene by contacting the cell in culture with a polynucleotide (that contains the target gene) attached to a translocating polypeptide. In another embodiment, expression of a target gene product in a cell in culture that contains a target gene under control of one or more regulatory elements is modulated by contacting the cell in culture with one or more regulatory agents attached to a translocating polypeptide. The one or more regulatory agents are translocated into the cell in culture and interact therein with the one or more regulatory elements to modulate expression of the target gene product by the cell.

摘要翻译： 本发明提供了通过使培养物中的细胞与连接于易位多肽的细胞过程修饰分子接触来调节细胞过程的方法。 例如，在一个实施方案中，通过使培养中的细胞与附着于易位多肽的多核苷酸（含有靶基因）接触，用靶基因转染培养细胞。 在另一个实施方案中，通过使培养物中的细胞与连接于易位多肽的一种或多种调节剂接触来调节目标基因产物在含有在一种或多种调节元件控制下的靶基因的培养物中的细胞中的表达。 一种或多种调节剂在培养物中被转运到细胞中并与其中的一种或多种调节元件相互作用以调节细胞对靶基因产物的表达。

公开(公告)号：US20050118717A1

公开(公告)日：2005-06-02

申请号：US10617910

申请日：2003-07-10

申请人： Gillian Elliott

发明人： Gillian Elliott

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/16 ,  A61P33/02 ,  A61P35/00 ,  C07K14/035 ,  A61K48/00 ,  C12N15/869

CPC分类号： A61K38/162 ,  C07K14/005 ,  C12N2710/16622

摘要： Herpesviral VP22 proteins (and variants) are used to modify cell structure and cell division, by their newly found property of binding to microtubules in cells. Uses of VP22 to exploit this property include stabilisation of animal cellular microtubules in vivo and in vitro, e.g. to retard or arrest cell division or induce cell death. The microtubule binding function of VP22 can be exploited by reagent use in vitro to study microtubules or the cell cycle particularly at cell division, and pharmaceutically to retard or arrest cell division of cells such as neoplastic cells or protozoal parasite cells in vitro or in vivo.

摘要翻译： 疱疹病毒VP22蛋白（和变体）用于通过其新发现的与细胞中微管结合的性质来修饰细胞结构和细胞分裂。 使用VP22来开发这种性质包括在体内和体外稳定动物细胞微管，例如。 延缓或阻止细胞分裂或诱导细胞死亡。 VP22的微管结合功能可以通过体外试剂用于研究微管或细胞周期，特别是细胞分裂，并药学上可以在体外或体内延缓或阻止细胞如肿瘤细胞或原生动物寄生虫细胞分裂。

公开(公告)号：US06893840B2

公开(公告)日：2005-05-17

申请号：US09977066

申请日：2001-10-12

申请人： Kent B. Thudium ,  Mark Selby

发明人： Kent B. Thudium ,  Mark Selby

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N7/00 ,  C12N15/67 ,  C12N15/85 ,  C12P21/02 ,  C12P21/06 ,  C07H21/04 ,  C12N5/00 ,  C12N15/00 ,  C12Q1/68

CPC分类号： C12N15/85 ,  C12N7/00 ,  C12N15/67 ,  C12N2710/16622 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2840/44 ,  C12N2840/445

摘要： Cytomegalovirus (CMV) Intron A fragments for expressing gene products are disclosed. Also described are expression vectors including the fragments, as well as methods of using the same.

摘要翻译： 公开了巨细胞病毒（CMV）用于表达基因产物的内含子A片段。 还描述了包括片段的表达载体，以及使用该片段的方法。

公开(公告)号：US20050089531A1

公开(公告)日：2005-04-28

申请号：US10398696

申请日：2001-10-05

申请人： Kazuo Kawakami ,  Masahiko Kishi ,  Masami Mochizuki

发明人： Kazuo Kawakami ,  Masahiko Kishi ,  Masami Mochizuki

IPC分类号： C12N15/09 ,  A61K39/245 ,  A61K48/00 ,  A61P31/04 ,  A61P31/12 ,  A61P31/22 ,  C07K14/035 ,  C07K14/04 ,  C12N7/04 ,  C12N15/38 ,  C12N15/869 ,  C12R1/93 ,  A61K39/12 ,  C12N7/00

CPC分类号： C12N7/00 ,  A61K48/00 ,  A61K2039/525 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/16622 ,  C12N2710/16722 ,  C12N2710/16743 ,  C12N2710/16761

摘要： An attenuated feline recombinant herpesvirus 1 (FHV-1), which is prepared by identifying gene regions in the genome wherein inserted foreign genes can be expressed without affecting the replication of FHV-1 and has least two types of foreign nucleic acid sequences inserted thereinto, usable as a vector virus or a vaccine. In this attenuated recombinant FHV-1, at least two types of foreign genes are inserted in such a manner as allowing the expression into two different gene regions exerting no lethal effect on the proliferation of the virus in the feline herpesvirus 1 genome.

摘要翻译： 一种减毒的猫重组疱疹病毒1（FHV-1），其通过鉴定基因组中的基因区域而制备，其中可以表达插入的外来基因而不影响FHV-1的复制，并且其中插入有至少两种类型的外源核酸序列， 可用作载体病毒或疫苗。 在这种减毒的重组FHV-1中，插入至少两种类型的外来基因，使得能够表达两个不同的基因区域，对猫疱疹病毒1基因组中病毒的增殖没有致死作用。

公开(公告)号：US06821519B2

公开(公告)日：2004-11-23

申请号：US10237551

申请日：2002-09-06

申请人： Craig H. Day ,  Nancy A. Hosken ,  Joseph M. Parsons

发明人： Craig H. Day ,  Nancy A. Hosken ,  Joseph M. Parsons

IPC分类号： A61K39245

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/53 ,  C07K2319/00 ,  C12N2710/16622 ,  G01N33/56994 ,  G01N2333/035

摘要： Compounds and methods for the diagnosis and treatment of HSV infection are provided. The compounds comprise polypeptides that contain at least one antigenic portion of an HSV polypeptide and DNA sequences encoding such polypeptides. Pharmaceutical compositions and vaccines comprising such polypeptides or DNA sequences are also provided, together with antibodies directed against such polypeptides. Diagnostic kits are also provided comprising such polypeptides and/or DNA sequences and a suitable detection reagent for the detection of HSV infection in patients and in biological samples.

摘要翻译： 提供了用于诊断和治疗HSV感染的化合物和方法。 所述化合物包含含有HSV多肽的至少一个抗原部分的多肽和编码此类多肽的DNA序列。 还提供了包含此类多肽或DNA序列的药物组合物和疫苗以及针对这些多肽的抗体。 还提供了诊断试剂盒，其包含这样的多肽和/或DNA序列和用于在患者和生物样品中检测HSV感染的合适的检测试剂。