公开(公告)号：US20050208657A1

公开(公告)日：2005-09-22

申请号：US10865538

申请日：2004-06-09

Applicant: Brian Dalby ,  Robert Bennett

Inventor： Brian Dalby ,  Robert Bennett

IPC: A61K48/00 ,  C07K14/035 ,  C12N15/85 ,  C12N15/87 ,  C12N15/74

CPC classification number: C07K14/005 ,  A61K48/0025 ,  C07K2319/00 ,  C12N15/85 ,  C12N15/87 ,  C12N2710/16622 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2840/20

Abstract: The invention provides methods for modulating a cellular process by contacting a cell in culture with a cell process-modifying molecule attached to a translocating polypeptide. For example, in one embodiment, a cell in culture is transfected with a target gene by contacting the cell in culture with a polynucleotide (that contains the target gene) attached to a translocating polypeptide. In another embodiment, expression of a target gene product in a cell in culture that contains a target gene under control of one or more regulatory elements is modulated by contacting the cell in culture with one or more regulatory agents attached to a translocating polypeptide. The one or more regulatory agents are translocated into the cell in culture and interact therein with the one or more regulatory elements to modulate expression of the target gene product by the cell.

Abstract translation: 本发明提供了通过使培养物中的细胞与连接于易位多肽的细胞过程修饰分子接触来调节细胞过程的方法。 例如，在一个实施方案中，通过使培养中的细胞与附着于易位多肽的多核苷酸（含有靶基因）接触，用靶基因转染培养细胞。 在另一个实施方案中，通过使培养物中的细胞与连接于易位多肽的一种或多种调节剂接触来调节目标基因产物在含有在一种或多种调节元件控制下的靶基因的培养物中的细胞中的表达。 一种或多种调节剂在培养物中被转运到细胞中并与其中的一种或多种调节元件相互作用以调节细胞对靶基因产物的表达。

公开(公告)号：US06773920B1

公开(公告)日：2004-08-10

申请号：US09937837

申请日：2002-01-07

Applicant: Brian Dalby ,  Robert P. Bennett

Inventor： Brian Dalby ,  Robert P. Bennett

IPC: C12N1587

CPC classification number: C07K14/005 ,  A61K48/0025 ,  C07K2319/00 ,  C12N15/85 ,  C12N15/87 ,  C12N2710/16622 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2840/20

Abstract: The invention provides methods for modulating a cellular process by contacting a cell in culture with a cell process-modifying molecule attached to a translocating polypeptide. For example, in one embodiment, a cell in culture is transfected with a target gene by contacting the cell in culture with a polynucleotide (that contains the target gene) attached to a translocating polypeptide. In another embodiment, expression of a target gene product in a cell in culture that contains a target gene under control of one or more regulatory elements is modulated by contacting the cell in culture with one or more regulatory agents attached to a translocating polypeptide. The one or more regulatory agents are translocated into the cell in culture and interact therein with the one or more regulatory elements to modulate expression of the target gene product by the cell.

Abstract translation: 本发明提供了通过使培养物中的细胞与连接于易位多肽的细胞过程修饰分子接触来调节细胞过程的方法。 例如，在一个实施方案中，通过使培养中的细胞与附着于易位多肽的多核苷酸（含有靶基因）接触，用靶基因转染培养细胞。 在另一个实施方案中，通过使培养物中的细胞与连接于易位多肽的一种或多种调节剂接触来调节目标基因产物在含有在一种或多种调节元件控制下的靶基因的培养物中的细胞中的表达。 一种或多种调节剂在培养物中被转运到细胞中并与其中的一种或多种调节元件相互作用以调节细胞对靶基因产物的表达。

公开(公告)号：US20120100522A1

公开(公告)日：2012-04-26

申请号：US13081436

申请日：2011-04-06

Applicant: Michael Saghbini ,  Michael Hogan ,  Chunnian Shi ,  Brian Dalby ,  David Wong

Inventor： Michael Saghbini ,  Michael Hogan ,  Chunnian Shi ,  Brian Dalby ,  David Wong

IPC: C12N5/078 ,  C12N1/20 ,  C12N5/071 ,  C12N7/00 ,  C12N1/00 ,  C09K3/00 ,  C12N1/14

CPC classification number: C12Q1/6806 ,  A01N1/021 ,  C07K17/02 ,  C12N1/04 ,  C12N9/96 ,  G01N33/54313

Abstract: The present invention provides compositions and methods that enable the stabilization and storage of samples by contacting a sample with an assembly of particles, and reducing the water activity level of the contacted sample. By reducing the water activity level of the sample, the assembly of particles minimizes the degradation of the sample. Stabilizers may or may not be added to the assembly of particles to further minimize the degradation of the sample. Subsequently to storage in the assembly of particles, the samples are recoverable by eluting the assembly of particles with a fluid solution. In one embodiment, the entire assembly of particles will dissolve into the solution. In another embodiment, only part of the assembly of particles will dissolve into the solution. The assembly of particles provides the advantage that while it is porous, it comprises non-porous particulate material.

Abstract translation: 本发明提供了通过使样品与颗粒组合接触并降低接触样品的水活度水平来使样品稳定和储存的组合物和方法。 通过降低样品的水分活度水平，颗粒的组装使样品的降解最小化。 稳定剂可以或可以不被添加到颗粒组合中以进一步使样品的降解最小化。 随后存储在颗粒的组装中，可以通过用流体溶液洗脱颗粒的组合来回收样品。 在一个实施例中，颗粒的整个组件将溶解到溶液中。 在另一个实施方案中，只有部分颗粒组合将溶解到溶液中。 颗粒的组合提供了这样的优点：当它是多孔的时，其包括无孔颗粒材料。