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223 条记录 (耗时 0.041 秒)

    Modified SELEX processes without purified protein
    62.
    发明授权
    Modified SELEX processes without purified protein 有权
    没有纯化蛋白质的修饰SELEX过程

    公开(公告)号：US06730482B2

    公开(公告)日：2004-05-04

    申请号：US10096641

    申请日：2002-03-12

    申请人： Larry Gold ,  Dominic A. Zichi ,  Jonathan Drew Smith

    发明人： Larry Gold ,  Dominic A. Zichi ,  Jonathan Drew Smith

    IPC分类号： C12Q168

    CPC分类号： C12N15/1048 ,  C12Q1/6811 ,  C12Q2523/313

    摘要： This invention is directed towards a method for obtaining nucleic acid ligands against target proteins without directly purifying the target proteins. The method used in the invention is called SELEX, which is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The nucleic acid ligands of the invention are useful as diagnostic and therapeutic agents for diseases in which the targets proteins play a causative role.

    摘要翻译： 本发明涉及一种在不直接纯化靶蛋白的情况下获得针对靶蛋白的核酸配体的方法。 本发明中使用的方法称为SELEX，它是通过扩充浓缩的配体的系统演化的缩写。 本发明的核酸配体可用作靶蛋白起作用的疾病的诊断和治疗剂。

    Method for the automated generation of nucleic acid ligands
    64.
    发明授权
    Method for the automated generation of nucleic acid ligands 失效
    自动生成核酸配体的方法

    公开(公告)号：US06716580B2

    公开(公告)日：2004-04-06

    申请号：US09815171

    申请日：2001-03-22

    申请人： Larry Gold ,  Dominic A. Zichi ,  Robert D. Jenison ,  Daniel J. Schneider

    发明人： Larry Gold ,  Dominic A. Zichi ,  Robert D. Jenison ,  Daniel J. Schneider

    IPC分类号： C12Q168

    CPC分类号： C12N15/1048

    摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a robotic manipulator controlled by a computer. The invention also includes methods and reagents to obviate the need for size-fractionation of amplified candidate nucleic acids before beginning the next round of the SELEX process.

    摘要翻译： 本发明包括用于执行自动化SELEX的方法和装置。 SELEX过程的步骤由工作表面上的一个或多个工作站由计算机控制的机器人操纵器执行。 本发明还包括在开始下一轮SELEX过程之前消除对扩增的候选核酸的大小分级的需要的方法和试剂。

    Bivalent binding molecules of 7 transmembrane G protein-coupled receptors
    65.
    发明授权
    Bivalent binding molecules of 7 transmembrane G protein-coupled receptors 失效
    7跨膜G蛋白偶联受体的二价结合分子

    公开(公告)号：US06682886B1

    公开(公告)日：2004-01-27

    申请号：US09118525

    申请日：1998-07-17

    申请人： Larry Gold

    发明人： Larry Gold

    IPC分类号： C12Q168

    CPC分类号： G01N33/74 ,  C12Q1/6811 ,  G01N2500/00 ,  C12Q2541/101

    摘要： Described herein are methods for identifying and preparing bivalent binding molecules to 7 transmembrane G protein-coupled receptors. The methods disclosed herein are based on the SELEX method for generating high affinity nucleic acid ligands. SELEX is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The methods of this invention combine two or more binding domains to two or more different epitopes of the same 7 transmembrane G protein-coupled receptor. These bivalent binding molecules are useful as therapeutic and diagnostic agents.

    摘要翻译： 本文描述了用于鉴定和制备二价结合分子至7个跨膜G蛋白偶联受体的方法。 本文公开的方法基于用于产生高亲和力核酸配体的SELEX方法。 SELEX是指数浓缩的配体的系统演化的缩写。 本发明的方法将两个或多个结合结构域结合到相同的7跨膜G蛋白偶联受体的两个或多个不同的表位。 这些二价结合分子可用作治疗和诊断剂。

    Nucleic acid ligand diagnostic biochip
    67.
    发明授权
    Nucleic acid ligand diagnostic biochip 有权
    核酸配体诊断生物芯片

    公开(公告)号：US06458543B1

    公开(公告)日：2002-10-01

    申请号：US09723517

    申请日：2000-11-28

    申请人： Larry Gold ,  Dan Drolet ,  Dom Zichi ,  Sumedha Jayasena ,  Steve Creighton ,  Stanley Gill

    发明人： Larry Gold ,  Dan Drolet ,  Dom Zichi ,  Sumedha Jayasena ,  Steve Creighton ,  Stanley Gill

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/00 ,  Y10S977/924 ,  C12Q2565/514 ,  C12Q2565/101 ,  C12Q2541/101 ,  C12Q2525/205

    摘要： A Nucleic acid ligand “Biochip” is disclosed, consisting of a solid support to which one or more specific Nucleic acid ligands is attached in a spatially defined manner. Each Nucleic acid ligand binds specifically and avidly to a particular Target molecule contained within a Test mixture, such as a Bodily fluid. The Target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. In principle, the Biochip could be used to test any chemically complex mixture provided that Nucleic acid ligands to components suspected of being present in the mixture are attached to the Biochip. Thus, the Nucleic acid ligand Biochip will have a wider use in environmental testing, etc.

    摘要翻译： 披露了一种核酸配体“生物芯片”，由一种或多种特定的核酸配体以空间限定的方式连接的固体支持物组成。 每个核酸配体特异性地和特异性地结合到包含在测试混合物例如体液内的特定靶分子。 靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。 原则上，生物芯片可以用于测试任何化学复合物混合物，只要核酸配体与怀疑存在于混合物中的组分连接在Biochip上即可。 因此，核酸配体生物芯片将在环境测试等方面有更广泛的应用。

    Transcription-free selex
    68.
    发明授权
    Transcription-free selex 失效
    无转录检查

    公开(公告)号：US06387620B1

    公开(公告)日：2002-05-14

    申请号：US09362578

    申请日：1999-07-28

    申请人： Jonathan Drew Smith ,  Larry Gold

    发明人： Jonathan Drew Smith ,  Larry Gold

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6811 ,  C12Q2537/155 ,  C12Q2531/113 ,  C12Q2521/501

    摘要： Methods are provided for the production of nucleic acid ligands against target molecules using a procedure known as Transcription-free Systematic Evolution of Ligands by EXponential enrichment (Transcription-free SELEX). The Transcription-free SELEX method assembles nucleic acid ligands from fragments of synthetic nucleic acids by annealing those fragments to a complementary template, and then ligating the fragments together.

    摘要翻译： 提供了使用已知为通过扩增浓缩（无转录的SELEX）的配体的无转录系统进化的方法生产针对靶分子的核酸配体的方法。 无转录的SELEX方法通过将这些片段退火成互补模板，然后将片段连接在一起，将合成核酸片段的核酸配体组装起来。

    Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
    69.
    发明授权
    Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction 有权
    通过核酸配体 - 配体信标相互作用均匀检测靶

    公开(公告)号：US06261783B1

    公开(公告)日：2001-07-17

    申请号：US09581326

    申请日：2000-08-11

    申请人： Sumedha Jayasena ,  Larry Gold

    发明人： Sumedha Jayasena ,  Larry Gold

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  C12Q1/6818 ,  C12Q1/682 ,  Y10T436/143333 ,  C12Q2561/12 ,  C12Q2537/101

    摘要： A method for detecting a target molecule in a test mixture suspected of containing said target molecule is described. The nucleic acid ligand capable of binding to the target molecule has a first sequence A and a second sequence B, which are partially complementary sequences that form an imperfect intramolecular duplex, which unwinds upon the binding of the target to the nucleic acid ligand. Sequences A and B are able to participate in extramolecular hybridization reactions only when the duplex is unwound. Three different cascade nucleic acids contain a first sequence and a second sequence, which are partially complementary sequences. At least one sequence is exactly complementary to A or B. The second sequence may be complementary to A or B, or may be a third sequence C, or its complement. The test mixture suspected of containing the target molecule is contacted with the nucleic acid ligand, causing the duplex of the nucleic acid ligand to unwind such that sequences A and B become available for extramolecular hybridization. This mixture is contacted with the first, second, and third cascade nucleic acids so that the unpaired A and B sequences triggers a cascade of intermolecular hybridization involving the cascade nucleic acids in which intermolecular hybridization takes place between A and its complement, B and its complement, and between C its complement, leading to the formation of a multimolecular hybridization complex. The presence of the multimolecular hybridization complex is then detected.

    摘要翻译： 描述了一种用于检测怀疑含有所述靶分子的试验混合物中的靶分子的方法。 能够结合靶分子的核酸配体具有第一序列A和第二序列B，它们是形成不完全分子内双链体的部分互补序列，其解离靶核酸与核酸配体的结合。 序列A和B只有当双链体解卷时才能参与分子内杂交反应。 三种不同的级联核酸含有第一序列和第二序列，其是部分互补序列。 至少一个序列与A或B完全互补。第二个序列可以与A或B互补，或者可以是第三个序列C或其补体。 怀疑含有靶分子的试验混合物与核酸配体接触，导致核酸配体的双链体解开，使得序列A和B可用于分子内杂交。 该混合物与第一级，第二级和第三级联核酸接触，使得不成对的A和B序列引发级联的分子间杂交，其涉及级联核酸，其中分子间杂交发生在A与其补体之间，B及其补体 ，和C之间的补体，导致形成多分子杂交复合物。 然后检测多分子杂交复合物的存在。