公开(公告)号：US20040073967A1

公开(公告)日：2004-04-15

申请号：US10601084

申请日：2003-06-20

Applicant: Universiteit Leiden

Inventor： Paul Jan, J. Hooykaas ,  Haico van Attikum ,  Paul Bundock

IPC: A01H001/00 ,  C12N015/82

CPC classification number: C12N15/8213 ,  C12N15/81 ,  C12N15/902 ,  C12N15/905

Abstract: The invention relates to methods for directing integration of a nucleic acid of interest towards homologous recombination and uses thereof. The present invention discloses factors involved in integration of a nucleic acid by illegitimate recombination which provides a method of directing integration of a nucleic acid of interest to a predetermined site, whereby the nucleic acid has a homology at or around the predetermined site, in a eukaryote with a preference for non-homologous recombination comprising steering an integration pathway towards homologous recombination. Furthermore, the invention provides a method of directing integration of a nucleic acid of interest to a subtelomeric and/or telomeric region in a eukaryote with a preference for non-homologous recombination.

Abstract translation: 本发明涉及用于指导目的核酸整合以同源重组及其用途的方法。 本发明公开了通过非法重组整合核酸所涉及的因素，其提供了将感兴趣的核酸整合到预定位点的方法，由此核酸在预定位点处或周围在真核生物中具有同源性 优选非同源重组，包括转向同源重组的整合途径。 此外，本发明提供了将目的核酸整合到真核生物中的亚端粒和/或端粒区域并优先选择非同源重组的方法。

公开(公告)号：US12016918B2

公开(公告)日：2024-06-25

申请号：US16609136

申请日：2018-04-25

Applicant: UNIVERSITEIT LEIDEN ,  ACADEMISCH ZIEKENHUIS LEIDEN

Inventor： Dmitri V. Filippov ,  Geoffroy P. P. Gential ,  Gijsbert Van Der Marel ,  Ferry Ossendorp

IPC: A61K39/39 ,  A61K47/64 ,  C07K5/062 ,  A61K39/00 ,  A61K45/06

CPC classification number: A61K39/39 ,  A61K47/64 ,  C07K5/0606 ,  A61K2039/55516 ,  A61K45/06

Abstract: Adjuvant compounds are described, as well as their conjugates comprising peptide antigens or other immunomodulatory moieties. Also described are methods of modulating immune responses in a subject in need thereof, as well as methods of treating cancer, viral or bacterial infections comprising administering the adjuvant compounds or the conjugates to a subject in need thereof.

公开(公告)号：US20230025535A1

公开(公告)日：2023-01-26

申请号：US17786238

申请日：2020-12-21

Applicant: UNIVERSITEIT LEIDEN

Inventor： Pauline Marthe Gerardina VAN DEURSEN ,  Gregory Fabrice SCHNEIDER ,  Alexander KROS ,  Viorica TUDOR

IPC: G01N23/2202 ,  G01N1/28 ,  H01J37/20

Abstract: A thin film liquid cell suitable for transmission electron microscopy at room temperature is fabricated as follows. A thin film floating on a liquid is prepared. A droplet of the liquid with the thin film floating thereon is transferred to a support by means of a loop. The loop carries the droplet and the droplet carries the thin film during this transfer. Sufficient liquid from the droplet on the support is removed to form the thin film liquid cell.

公开(公告)号：US11378513B2

公开(公告)日：2022-07-05

申请号：US17052472

申请日：2019-05-03

Applicant: UNIVERSITEIT LEIDEN

Inventor： Frans Snik ,  Christoph Ulrich Keller

IPC: G01N21/21 ,  G01N21/31 ,  G01N21/17

Abstract: Apparatus (100) for determining presence of a gas (103) is provided, the apparatus (100) comprising: one or more retarders (109) to spectrally modulate polarisation of received radiation in accordance with a plurality of polarised spectral modulation profiles which are offset in phase from each other, the radiation output from the one or more retarders (109) comprising radiation having polarisation spectrally modulated in accordance with the said plurality of polarised spectral modulation profiles in a common beam of radiation; one or more polarisers (147, 148); and radiation detectors (142, 144) to detect radiation output from the one or more retarders (109) filtered for respective polarisation states by the one or more polarisers (147, 148), the detectors (142, 144) selectively and separately detecting on different detectors at the same time polarised radiation conforming to each of at least first and second of the said polarised spectral modulation profiles to thereby provide at least respective first and second polarisation-dependent radiation intensity measurements from which the presence of the gas (103) can be determined.

公开(公告)号：US11344877B2

公开(公告)日：2022-05-31

申请号：US14426920

申请日：2013-09-10

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Ender Yildirim

IPC: B01L3/00

Abstract: The present invention relates to an apparatus for controlling the shape and/or position of a moveable fluid-fluid meniscus, and methods of use, in particular a method to control the shape of a moveable fluid-fluid meniscus in an apparatus in which the meniscus is caused to align along a stable capillary barrier or phaseguide.

公开(公告)号：US10746824B2

公开(公告)日：2020-08-18

申请号：US16081210

申请日：2017-03-01

Applicant: UNIVERSITEIT LEIDEN

Inventor： Arthur den Haan ,  Jelmer Wagenaar ,  Tjerk Oosterkamp ,  Rembrandt Donkersloot

IPC: G01R33/32 ,  G01N24/08 ,  G01N24/10 ,  G01R33/038 ,  G01Q60/52 ,  G01R33/60

Abstract: A magnetic resonance force detection apparatus, comprising: a sample carrier for carrying a sample to be tested; a magnetic field source configured to provide a magnetic field to a sample when it is carried by the sample carrier; a support for supporting either the sample carrier or the magnetic field source; a support-driving-mechanism configured to drive the support such that the sample carrier moves relative to the magnetic field source, such that the magnetic field is configured to cause the spins of one or more nuclei or electrons in the sample to flip, and wherein the flipping of spins exerts a force on the support; and a support-displacement-measuring-sensor configured to measure displacement of the support and generate a signal representative of the displacement of the support.

公开(公告)号：US10746745B2

公开(公告)日：2020-08-18

申请号：US15868201

申请日：2018-01-11

Applicant: Academisch Ziekenhuis Leiden ,  Universiteit Leiden

Inventor： Ericus Anna Leonardus Biessen ,  Theodorus Josephus Cornelis Van Berkel ,  Adriaan Otto Kraaijeveld

IPC: G01N33/53 ,  A61P9/04 ,  A61P9/10 ,  G01N33/68 ,  C12Q1/6883 ,  H01J49/00 ,  H01J49/16 ,  H01J49/40 ,  C07K14/52 ,  C07K16/24

Abstract: The present invention relates to the identification of chemokine biomarkers predictive of future acute coronary syndromes including unstable angina pectoris (UAP). The present invention also identifies particular chemokines as potential therapeutic targets for intervention in cardiovascular diseases.

公开(公告)号：US20200071650A1

公开(公告)日：2020-03-05

申请号：US16571552

申请日：2019-09-16

Applicant: UNIVERSITEIT LEIDEN

Inventor： Paul Vulto ,  Sebastiaan Johannes Trietsch ,  Heiko Jan van der Linden ,  Adrianus Theodorus Joore ,  Thomas Hankemeier

IPC: C12M1/00 ,  B01L3/00 ,  C12M1/42 ,  C12M3/06 ,  B01F13/00

Abstract: Apparatus for processing life-based organic particles, including particles selected from the list comprising cells, cellular spheroids, tissues, eukaryotes, micro-organisms, organs or embryos, comprises a hollow volume (10) that (a) is internally divided into at least first (14), second (16) and third (17) sub-volumes by at least two phaseguides (12, 13) formed inside the volume and (b) includes parts that are relatively upstream and relatively downstream when judged with reference to the movement of a meniscus or a bulk liquid in the volume (10). The apparatus includes at least first, second and third fluid conduits (19, 21, 22) connected to permit fluid communication between the upstream exterior of the volume (10) and a respective said sub-volume (14, 16, 17); and at least one further conduit (24) connected to permit fluid communication between the downstream exterior of the volume (10) and a said sub-volume. The first sub-volume (14) contains one or more life-based particles supported in or by a gel or gel-like substance; and the second sub-volume (16) communicates with the first sub-volume so as to permit transport of substances between the first and second sub-volumes (14, 16) and contains at least one gel or gel-like substance.

公开(公告)号：US20190390208A1

公开(公告)日：2019-12-26

申请号：US16087199

申请日：2017-03-23

Applicant: Universiteit Leiden ,  Academisch Ziekenhuis Leiden h.o.d.n. LUMC

Inventor： Marcel TIJSTERMAN ,  Maartje VAN KREGTEN ,  Paul HOOYKAAS

IPC: C12N15/82

Abstract: The present disclosure provides methods for transfecting plants and for expressing RNA or polypeptide molecules in plants. In particular, plants having reduced POLQ expression and/or activity are transfected in order to reduce random integration events. The disclosure further provides transfected plants and plant progeny produced by the methods disclosed herein.

公开(公告)号：US10222318B2

公开(公告)日：2019-03-05

申请号：US15510104

申请日：2015-09-09

Applicant: Universiteit Leiden ,  Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.

Inventor： Sanli Ebrahimi Pour Faez ,  Vahid Sandoghdar

IPC: G01N15/14 ,  G01N21/53 ,  G01N21/47 ,  G01N21/64 ,  B01L3/00 ,  G01N15/00

Abstract: There is provided a particle detection apparatus (30) comprising: a channel (32) including an inlet and at least one channel wall, the inlet permitting light to be introduced into the channel (32), the or each channel wall being arranged to define a channel path through which light may propagate; a light source (34) configured to introduce light into the channel (32) via the inlet, the channel (32) being shaped to guide the light to propagate along the channel path for illuminating a particle or a plurality of particles located in the channel path; and a monitoring device (36) configured to detect scattered light that is created by the illumination of the or each particle by the guided light and that leaves the channel (32) by passing through the or each channel wall.