公开(公告)号：US11454618B2

公开(公告)日：2022-09-27

申请号：US16506280

申请日：2019-07-09

申请人： MLS ACQ, INC.

发明人： Martin L. Spartz ,  Anthony S. Bonanno ,  Peter P. Behnke

IPC分类号： G01N30/74 ,  G01N30/20 ,  G01N30/88 ,  G01N30/02 ,  G01N30/06 ,  G01N30/30 ,  G01N30/38 ,  G01N30/08

摘要： A system and method are disclosed for analyzing samples, which includes a spectrometry system for detecting components of a sample; a gas chromatography column for separating the components of a sample; a first sample unit for receiving a first sample from a sample source; and a second sample unit for receiving a second sample from a sample source. Each sample loop unit allows independent processing of samples in preparation for analysis.

公开(公告)号：US11415583B2

公开(公告)日：2022-08-16

申请号：US16675109

申请日：2019-11-05

申请人： National University Corporation Nagoya University ,  Hitachi, Ltd.

发明人： Hiroo Uchida ,  Akinari Hinoki ,  Minoru Sakairi ,  Mayumi Abe

IPC分类号： G01N33/574 ,  G16H50/30 ,  G16B40/10 ,  G01N30/06 ,  G01N30/02

摘要： Provided are a method, an apparatus, and a kit for detecting a neuroblastoma in a subject and/or for monitoring a therapeutic effect on the neuroblastoma, by measuring a urinary tumor marker(s) in a sample from the subject.

公开(公告)号：US20220196671A1

公开(公告)日：2022-06-23

申请号：US17554784

申请日：2021-12-17

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Andrew Kleinberg ,  Yuan Mao

IPC分类号： G01N33/68 ,  B01D15/16 ,  B01D15/30 ,  G01N30/72 ,  G01N30/06

摘要： Disclosed are methods for identification of one or more non-native disulfide bonds in a biomolecule (e.g, an antibody). In an example, a method includes performing a digestion of the biomolecule under non-reducing conditions to provide a sample comprising a plurality of biomolecule fragments, contacting the sample to a separation column, applying a first mobile phase gradient comprising trifluoroacetic acid (TFA) and a small molecule additive to the separation column, applying a second mobile phase gradient comprising TFA in acetonitrile (ACN) and a small molecule additive to the separation column, performing a partial reduction procedure on the eluted sample, applying the partially reduced eluted sample components to a mass spectrometer, and performing a mass spectrometric analysis on the partially reduced eluted sample components to identify the one or more non-native disulfide bonds in the biomolecule.

公开(公告)号：US11365214B2

公开(公告)日：2022-06-21

申请号：US16462464

申请日：2017-07-12

申请人： DALIAN INSTITUTE OF CHEMICAL PHYSICS, CHINESE ACADEMY OF SCIENCES

发明人： Lihua Zhang ,  Huiming Yuan ,  Zhigang Sui ,  Kaiguang Yang ,  Yukui Zhang

IPC分类号： C07K1/14 ,  C12Q1/37 ,  G01N1/40 ,  G01N30/14 ,  G01N30/72 ,  G01N30/06

摘要： The invention relates to a method for the treatment of body fluid proteins, by which proteins from body fluids such as blood or urine are extracted by adding a certain proportion of high molecular polymer solution under low temperature condition followed by denaturation and reduction by adding a certain concentration of surfactant and tris(2-carboxyethyl) phosphine (TCEP) under a high temperature condition. Subsequently, the iodoacetic acid brushes grafted on silica microspheres called as solid-phase alkylation reagents are added into protein solution, which can react rapidly with the protein sulfhydryl group. After centrifugation, the microspheres are obtained and repeatedly washed with methanol and buffer to remove interferences such as sugars, salts, surfactants, lipids to obtain high-purity proteins, and finally protease is added to digest proteins into peptides. After centrifugation, the peptide products are obtained, and directly analyzed by liquid chromatography-mass spectrometry (LC-MS) system. Compared with the traditional protein pretreatment method, the method has many advantages such as good anti-interference capability, easy operation and short pretreatment time.

公开(公告)号：US11359213B2

公开(公告)日：2022-06-14

申请号：US16897617

申请日：2020-06-10

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Shunhai Wang

IPC分类号： C12N15/86 ,  C12N7/00 ,  G01N30/02 ,  C07K1/20 ,  G01N30/06

摘要： Methods of determining the stoichiometry of a viral capsid and/or determining the heterogeneity of protein components in a viral capsid are disclosed.

公开(公告)号：US11327060B2

公开(公告)日：2022-05-10

申请号：US15938450

申请日：2018-03-28

申请人： Ajinomoto Co., Inc.

发明人： Masashi Harada ,  Sachise Karakawa ,  Kazutaka Shimbo ,  Naoyuki Yamada

IPC分类号： G01N30/00 ,  G01N30/88 ,  C07C271/58 ,  C07D401/12 ,  C07D207/46 ,  C07D213/40 ,  G01N30/06 ,  G01N27/62 ,  B01D15/38 ,  C07C233/62 ,  C07B57/00 ,  B01D15/32 ,  C07C51/353 ,  C07C51/47 ,  C07C53/18 ,  C07C269/06 ,  C07C269/08 ,  G01N1/40 ,  G01N30/72

摘要： Enantiomers may be analyzed by: (1) reacting a mixture of a first compound and a second compound that are a pair of enantiomers with an axially chiral compound that is one of a pair of axially chiral isomers, to generate a derivative mixture containing a first derivative obtained by a reaction of the first compound with the axially chiral compound and a second derivative obtained by a reaction of the second compound with the axially chiral compound; (2) separating the first derivative and the second derivative in the derivative mixture; and (3) detecting the separated first derivative and second derivative by mass spectrometry.

公开(公告)号：US20220034771A1

公开(公告)日：2022-02-03

申请号：US16944622

申请日：2020-07-31

申请人： SHIMADZU CORPORATION

发明人： Ayaka MIYAMOTO ,  Toyohito WADA

IPC分类号： G01N1/44 ,  G01N30/06

摘要： An analytical device includes: a sample vaporization chamber into which a sample is to be injected and which, if the sample is a liquid sample, is configured to vaporize the liquid sample; a first temperature regulation unit and a second temperature regulation unit configured to regulate a temperature of the sample vaporization chamber; and a detection unit configured to detect the sample which is separated at a separation column that is connected with the sample vaporization chamber, the separation column being configured to separate the sample in a gas phase.

公开(公告)号：US20220026398A1

公开(公告)日：2022-01-27

申请号：US17309062

申请日：2019-10-21

申请人： Human Metabolomics Institute Inc.

发明人： Wei JIA ,  Guoxiang XIE ,  Lu WANG

IPC分类号： G01N30/06 ,  B01L3/00 ,  G01N30/72

摘要： The present invention discloses a quantitative detection method of multiple metabolic components in a biological sample and a metabolic chip used in the method. The detection method includes performing derivatization treatment on the biological sample and then detecting the derivatized biological sample by liquid chromatography-mass spectrometry. During derivatization treatment, 3-nitrophenylhydrazine is used as a derivatization reagent, and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide is used as a derivatization reaction catalyst. According to the detection method of the present invention, high-sensitivity detection can be achieved, multiple metabolic components of different magnitudes can be detected, the operation is simple and fast, and the method is applicable to clinical detection and scientific research examination. The metabolic chip of the present invention includes a chip carrier microtiter plate and related reagents, and quantitative detection of multiple metabolic components of different magnitudes such as amino acid, phenol, phenyl or benzyl derivative, indole, organic acid, fatty acid, sugar, and bile acid in the biological sample on the same microtiter plate can be achieved.

公开(公告)号：US20220011276A1

公开(公告)日：2022-01-13

申请号：US17358391

申请日：2021-06-25

申请人： Waters Technologies Corporation

发明人： Fabrice Gritti

IPC分类号： G01N30/06 ,  G01N30/16

摘要： A mixer arrangement for use in a chromatography system includes a first frequency targeting mixer including a first flow channel coupled between an inlet and an outlet and a second flow channel coupled between the inlet and the outlet, the second flow channel including a volume offset region configured to delay fluid propagation through the second flow channel, wherein the volume offset region is configured to reduce or eliminate fluidic compositional oscillations in a compositional solvent stream that depart from a desired composition at a first target frequency, and a residual noise targeting mixer fluidically connected in series to the frequency targeting mixer, the residual noise targeting mixer configured to dampen aperiodic baseline noise in the compositional solvent stream.

公开(公告)号：US11209449B2

公开(公告)日：2021-12-28

申请号：US15557345

申请日：2015-03-11

申请人： SHIMADZU CORPORATION

发明人： Masami Tomita

IPC分类号： G01N1/42 ,  G01N35/02 ,  G01N30/24 ,  G01N35/10 ,  B01D15/12 ,  B01D15/24 ,  G01N30/06 ,  G01N30/88 ,  G01N35/00

摘要： An autosampler includes: a sample cooling unit that is brought into thermally contact with a bottom surface of a sample rack so as to cool a sample accommodated in the sample rack; a condensed water receiver that has at least one hole on a bottom surface thereof, and is provided below the sample rack for receiving water condensed around the sample rack; a discharging passage configured in such a manner that a droplet falling from the at least one hole flows therein.