公开(公告)号：US20230407358A1

公开(公告)日：2023-12-21

申请号：US17800157

申请日：2021-02-18

Applicant: Translate Bio, Inc.

Inventor： Dustin Cooper ,  Frank DeRosa ,  Anusha Dias ,  Jeffrey S. Dubins ,  Xiaobo Gu ,  Khang Anh Tran

IPC: C12P19/34 ,  C12N9/12 ,  C12N15/10

CPC classification number: C12P19/34 ,  C12Y207/07006 ,  C12N9/1247 ,  C12N15/1068

Abstract: The present invention provides methods for preparing optimized DNA sequences as templates for in vitro transcription of mRNA. These DNA sequences are optimized to avoid premature termination of transcription by RNA polymerase. The invention also provides methods for preparing optimized DNA sequences that include one or more termination signal at their 3′ end to reduce or prevent non-templated “runoff” transcription.

公开(公告)号：US11834711B2

公开(公告)日：2023-12-05

申请号：US17484674

申请日：2021-09-24

Applicant: Karius, Inc.

Inventor： Timothy A Blauwkamp ,  Rene Sit ,  Igor D. Vilfan

IPC: C12Q1/6869 ,  C12N9/00 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12N9/12 ,  C12Q1/686 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12N9/1247 ,  C12N9/93 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6876 ,  C12Q2521/107 ,  C12Q2533/101 ,  C12Y207/07006 ,  C12Y207/07049 ,  C12Y605/01001 ,  C12Y605/01003 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2535/122

Abstract: The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

公开(公告)号：US20230348946A1

公开(公告)日：2023-11-02

申请号：US18221364

申请日：2023-07-12

Applicant: MICROSOFT TECHNOLOGY LICENSING, LLC

Inventor： Bichlien Hoang NGUYEN

IPC: C12P19/36 ,  C12Q1/6874 ,  B01J19/00

CPC classification number: C12P19/36 ,  B01J19/0046 ,  C12Q1/6874 ,  C12Y207/07031 ,  C12P19/34 ,  C12Y207/07006

Abstract: Polynucleotide synthesis performed with a substrate independent polymerase such as terminal deoxynucleotidyl transferase (TdT) is regulated by controlling the oxidation state of a metal cofactor. The oxidation state of the metal cofactor is changed to +2, thus activating the polymerase, by applying a voltage with electrodes or by introducing a chemical redox reagent. Addressable polynucleotide synthesis creates polynucleotides with different arbitrary sequences through use of spatial control of cofactor oxidation states to add nucleotides only at selected locations on an array. Control of metal oxidation states is regulated by selective activation of a microelectrode array, controlled addition of redox reagents to specific locations on the array, or controlled activation of photocatalysts at specific locations on the array. Scavengers in solution prevent cofactors distant from the selected locations from catalyzing polymerase activity and thereby maintain the localized effect of polymerase activation.

公开(公告)号：US20190211368A1

公开(公告)日：2019-07-11

申请号：US15768206

申请日：2016-10-17

Applicant: ModernaTX, Inc.

Inventor： Gabor BUTORA ,  Matthew STANTON ,  Edward John MIRACCO

IPC: C12P19/34 ,  C12N15/113 ,  C07H21/02 ,  C12N15/10 ,  C12N9/12 ,  A61K31/7125

CPC classification number: C12P19/34 ,  A61K31/7125 ,  C07H21/02 ,  C12N9/1247 ,  C12N15/1096 ,  C12N15/113 ,  C12N2310/317 ,  C12Y207/07006

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the novel cap analogs.

公开(公告)号：US09988612B2

公开(公告)日：2018-06-05

申请号：US15127617

申请日：2015-03-20

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Andrew D. Ellington ,  Adam J. Meyer

IPC: C12N9/12 ,  C12P19/34 ,  C12N15/115 ,  C12N15/11

CPC classification number: C12N9/1247 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/141 ,  C12N2310/16 ,  C12N2320/51 ,  C12N2330/00 ,  C12N2330/51 ,  C12P19/34 ,  C12Y207/07006

Abstract: Disclosed are T7 RNA polymerase variants with enhanced transcriptional activity. T7 RNA polymerase variants are known which have the ability to incorporate modified ribonucleotides into growing RNA molecules. However, these variants have relatively low levels of transcriptional activity. Presented herein are mutations that increase the transcriptional activity of the variants with broad substrate range.

公开(公告)号：US20170298367A1

公开(公告)日：2017-10-19

申请号：US15514028

申请日：2015-09-21

Applicant: UNIVERSITE DE BOURGOGNE ,  INRA ,  INSERM

Inventor： Fabrice NEIERS ,  Renaud SEIGNEURIC ,  Loïc BRIAND ,  Carmen GARRIDO-FLEURY

IPC: C12N15/72 ,  C12N15/74 ,  C12N9/12 ,  C12N1/20 ,  C12P21/00 ,  C07K14/47

CPC classification number: C12N15/72 ,  C07K14/47 ,  C12N1/20 ,  C12N9/1247 ,  C12N15/74 ,  C12P21/00 ,  C12P21/02 ,  C12Y207/07006

Abstract: A method for the expression of a protein of interest by a bacterium, notable in that it comprises the culturing of a bacterium temporarily or continuously expressing an Hsp protein, in that said bacterium also comprises a nucleic acid sequence, encoding a protein of interest, under the control of a lac promoter and in that said bacterium is cultured in a medium which does not contain IPTG or a metabolized molecule in such a way as to automatically induce the induction of transcription from the lac promoter.

公开(公告)号：US20170233708A1

公开(公告)日：2017-08-17

申请号：US15518639

申请日：2015-10-22

Applicant: President and Fellows of Harvard College

Inventor： David R. LIU ,  Bryan DICKINSON ,  Michael S. PACKER ,  Ahmed Hussein BADREN

IPC: C12N9/50 ,  C12N9/12 ,  C12N15/62 ,  C12N7/00 ,  C12N15/86 ,  C12N15/52

CPC classification number: C12N9/50 ,  C07K2319/50 ,  C12N7/00 ,  C12N9/1247 ,  C12N15/1037 ,  C12N15/1058 ,  C12N15/52 ,  C12N15/62 ,  C12N15/86 ,  C12N2795/00022 ,  C12Y207/07006 ,  C12Y304/00

Abstract: Some aspects of this disclosure provide methods for phage-assisted continuous evolution (PACE) of proteases. Some aspects of this invention provide methods for evaluating and selecting protease inhibitors based on the likelihood of the emergence of resistant proteases as determined by the protease PACE methods provided herein. Some aspects of this disclosure provide strategies, methods, and reagents for protease PACE, including fusion proteins for translating a desired protease activity into a selective advantage for phage particles encoding a protease exhibiting such an activity and improved mutagenesis-promoting expression constructs. Evolved proteases that recognize target cleavage sites which differ from their canonical cleavage site are also provided herein.

公开(公告)号：US20170211050A1

公开(公告)日：2017-07-27

申请号：US15127617

申请日：2015-03-20

Applicant: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM

Inventor： Andrew D. Ellington ,  Adam J. Meyer

IPC: C12N9/12 ,  C12N15/115 ,  C12N15/11 ,  C12P19/34

CPC classification number: C12N9/1247 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/141 ,  C12N2310/16 ,  C12N2320/51 ,  C12N2330/00 ,  C12N2330/51 ,  C12P19/34 ,  C12Y207/07006

Abstract: Disclosed are T7 RNA polymerase variants with enhanced transcriptional activity. T7 RNA polymerase variants are known which have the ability to incorporate modified ribonucleotides into growing RNA molecules. However, these variants have relatively low levels of transcriptional activity. Presented herein are mutations that increase the transcriptional activity of the variants with broad substrate range.

公开(公告)号：US20170183635A1

公开(公告)日：2017-06-29

申请号：US15449827

申请日：2017-03-03

Applicant: The Regents of the University of California

Inventor： William M. Gelbart ,  Charles M. Knobler ,  Rees F. Garmann ,  Odisse Azizgolshani ,  Ruben D. Cadena-Nava

IPC: C12N7/00 ,  C12N9/12

CPC classification number: C12N7/00 ,  C07K14/005 ,  C12N7/045 ,  C12N9/1247 ,  C12N2770/14022 ,  C12N2770/14023 ,  C12N2770/14033 ,  C12N2770/14042 ,  C12Y207/07006

Abstract: The invention provides compositions of matter comprising a cowpea chlorotic mottle virus capsid protein (CCMV CP) and a ribonucleic acid, as well as methods for using such compositions. In such compositions, the cowpea chlorotic mottle virus capsid protein envelops the ribonucleic acid so as to for a capsid that can inhibit the degradation of the ribonucleic acid (e.g. by RNAses). A method of delivering a ribonucleic acid into the cytoplasm of a mammalian cell is also provided. Typically, the method comprises the steps of combining the mammalian cell with a composition of matter described herein under conditions selected to allow the cowpea chlorotic mottle virus capsid to contact the mammalian cell and deliver the ribonucleic acid into the cytoplasm of a mammalian cell.

公开(公告)号：US20170130255A1

公开(公告)日：2017-05-11

申请号：US15356132

申请日：2016-11-18

Applicant: ModernaTX, Inc.

Inventor： Yuxun Wang ,  Divakar Ramakrishnan ,  Antonin De Fougerolles ,  Susan Whoriskey

IPC: C12P19/34 ,  C12N9/12

CPC classification number: C12P19/34 ,  C12N9/1241 ,  C12N9/1247 ,  C12N9/1252 ,  C12Y207/07006 ,  C12Y207/07007 ,  C12Y207/0705

Abstract: The invention relates to compositions and methods for the design, evolution, preparation, and/or manufacture of enzymes for use with polynucleotides, primary transcripts and mmRNA molecules.