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公开(公告)号：US20120271033A1
公开(公告)日：2012-10-25
申请号：US13497332
申请日：2010-10-05
Applicant: Albert Collinson , Peter Wagner , Matti Sällberg , Anders Vahlne , Gregor Schürmann , Robert Kamen
Inventor： Albert Collinson , Peter Wagner , Matti Sällberg , Anders Vahlne , Gregor Schürmann , Robert Kamen
CPC classification number: C07K14/00 , C12N15/1062 , C40B30/04 , C40B40/08 , C40B50/06
Abstract: Disclosed are methods for identifying high affinity adaptor molecules that bind to both a circulating antibody and a target molecule and redirect the specificity of the circulating antibody to the target molecule. Exemplary high affinity adaptor molecules are also provided.
Abstract translation: 公开了用于鉴定结合循环抗体和靶分子并将循环抗体的特异性重定向到靶分子的高亲和性衔接子分子的方法。 还提供了示例性的高亲和性衔接子分子。
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公开(公告)号：US08288123B2
公开(公告)日：2012-10-16
申请号：US11326710
申请日：2006-01-05
Applicant: Andrew Buchanan , Lutz Jermutus
Inventor： Andrew Buchanan , Lutz Jermutus
CPC classification number: C12N15/1062 , C12N15/1041
Abstract: A method of providing a subject polypeptide variant with improved stability compared with a parent subject polypeptide, employing translation and selection using an RNA expression system, wherein two or more stability selection pressures are applied simultaneously during translation, two or more stability selection pressures are applied simultaneously during selecting, or at least one stability selection pressure is applied during translation and continues to be applied during selecting, and at least one further stability selection pressure is applied during selecting.
Abstract translation: 提供与亲本受试者多肽相比具有改善的稳定性的受试者多肽变体的方法，其使用使用RNA表达系统的翻译和选择，其中在翻译期间同时施加两个或更多个稳定性选择压力，同时施加两个或更多稳定性选择压力 或在选择期间施加至少一个稳定性选择压力，并且在选择期间继续施加稳定性选择压力，并且在选择期间施加至少一个其它稳定性选择压力。
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公开(公告)号：US20120208720A1
公开(公告)日：2012-08-16
申请号：US13502487
申请日：2010-10-21
Applicant: Kenji Kashiwagi , Patrick C. Reid
Inventor： Kenji Kashiwagi , Patrick C. Reid
CPC classification number: C12N15/1062 , C40B40/08 , C40B50/06
Abstract: Provided are linkers suitable for preparing a conjugate of a nucleic acid and a peptide as a translation product thereof in a reconstituted cell-free translation system in genotype-phenotype mapping (display methods), said linkers comprising a single-stranded structure region having a side chain base pairing with the base at the 3′-end of an mRNA at one end and a peptidyl acceptor region containing an amino acid attached to an oligo RNA consisting of a nucleotide sequence of ACCA via an ester bond at the other end, characterized in that the ester bond is formed by using an artificial RNA catalyst. Also provided are display methods using [mRNA]-[linker]-[peptide] conjugates assembled via such linkers.
Abstract translation: 提供了适合于在基因型 - 表型定位（显示方法）的重建无细胞翻译系统中制备核酸和肽作为其翻译产物的缀合物的接头，所述接头包含具有侧面的单链结构区域 在一端的mRNA的3'-末端与碱基配对的链碱基和含有通过另一端通过酯键由ACCA的核苷酸序列连接的寡核苷酸连接的氨基酸的肽基受体区域，其特征在于 通过使用人造RNA催化剂形成酯键。 还提供了使用通过这种接头组装的[mRNA] - [接头] - [肽]缀合物的显示方法。
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公开(公告)号：US20060183200A1
公开(公告)日：2006-08-17
申请号：US11326710
申请日：2006-01-05
Applicant: Andrew Buchanan , Lutz Jermutus
Inventor： Andrew Buchanan , Lutz Jermutus
CPC classification number: C12N15/1062 , C12N15/1041
Abstract: A method of providing a subject polypeptide variant with improved stability compared with a parent subject polypeptide, employing translation and selection using an RNA expression system, wherein two or more stability selection pressures are applied simultaneously during translation, two or more stability selection pressures are applied simultaneously during selecting, or at least one stability selection pressure is applied during translation and continues to be applied during selecting, and at least one further stability selection pressure is applied during selecting.
Abstract translation: 提供与亲本受试者多肽相比具有改善的稳定性的受试者多肽变体的方法，其使用使用RNA表达系统的翻译和选择，其中在翻译期间同时施加两个或更多个稳定性选择压力，同时施加两个或更多稳定性选择压力 或在选择期间施加至少一个稳定性选择压力，并且在选择期间继续施加稳定性选择压力，并且在选择期间施加至少一个其它稳定性选择压力。
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35.发明申请公开(公告)号：US20050089913A1
公开(公告)日：2005-04-28
申请号：US10960453
申请日：2004-10-07
Applicant: Richard Williams
Inventor： Richard Williams
CPC classification number: C12N15/1034 , C12N15/1062 , G01N33/58 , G01N33/6845 , G01N2458/10
Abstract: This invention relates generally to systems and methods for identifying and selecting, desired proteins or nucleic acid molecules by linking mRNA, with known or unknown sequences, to its translated protein to form a cognate pair. The cognate pair is selected based upon desired properties of the protein or the nucleic acid. This method also includes the evolution of a desired protein or nucleic acid molecule by amplifying the nucleic acid portion of the selected cognate pair, introducing variation into the nucleic acid, translating the nucleic acid, attaching the nucleic acid to its protein to form a second cognate pair, and re-selecting this cognate pair based upon desired properties. Modified mRNAs operable to crosslink to tRNAs are also provided. Methods of producing a psoralen monoadduct or a crosslink are also provided.
Abstract translation: 本发明一般涉及通过将mRNA与已知或未知序列连接到其翻译的蛋白质以形成同源对来鉴定和选择所需蛋白质或核酸分子的系统和方法。 基于蛋白质或核酸的所需性质选择同源对。 该方法还包括通过扩增所选择的同源对的核酸部分，引入核酸的变异，翻译核酸，将核酸连接到其蛋白质以形成第二个同源物，从而演化期望的蛋白质或核酸分子 并且基于所需的属性重新选择该同源对。 还提供了可操作以交联至tRNA的修饰的mRNA。 还提供了补骨脂素单加成物或交联剂的制备方法。
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Patent Agency Ranking
公开(公告)号：US20050069920A1
公开(公告)日：2005-03-31
申请号：US10860750
申请日：2004-06-03
Applicant: Andrew Griffiths , Dan Tawfik
Inventor： Andrew Griffiths , Dan Tawfik
IPC: C12N15/09 , C12N9/02 , C12N15/10 , C12P17/18 , C12Q1/32 , C12Q1/68 , G01N33/15 , G01N33/50 , C12N1/08
CPC classification number: C12N15/1058 , C12N15/1062 , C12N15/1075 , C12Q1/6811 , C12Q2563/159
Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising of the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.
Abstract translation: 本发明描述了一种用于分离编码具有所需活性的基因产物的一种或多种遗传元件的方法，包括以下步骤：（a）将遗传元件分隔成微胶囊; （b）表达遗传元件以在微胶囊内产生它们各自的基因产物; （c）分选产生具有所需活性的基因产物的遗传元件。 本发明能够通过本发明方法的重复诱变和迭代应用来体外进化核酸。
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公开(公告)号：US20050037392A1
公开(公告)日：2005-02-17
申请号：US10866237
申请日：2004-06-11
Applicant: Andrew Griffiths , Dan Tawfik , Armin Sepp
Inventor： Andrew Griffiths , Dan Tawfik , Armin Sepp
IPC: G01N21/78 , C07K2/00 , C12N9/00 , C12N15/09 , C12N15/10 , C12P1/00 , C12P21/00 , C12P21/02 , C12Q1/00 , C12Q1/26 , C12Q1/68 , C12Q1/6811 , G01N21/64 , G01N33/15 , G01N33/50 , C12P19/34
CPC classification number: C12N15/1075 , C12N15/1062 , C12N15/113 , C12Q1/6811
Abstract: The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having the desired activity using a change in the optical properties of the genetic elements. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
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38.发明申请System for rapid identification and selection of nucleic acids and polypeptides, and method thereof 有权用于快速鉴定和选择核酸和多肽的系统及其方法公开(公告)号：US20040229270A1
公开(公告)日：2004-11-18
申请号：US10847087
申请日：2004-05-17
Inventor： Richard B. Williams
IPC: C12Q001/68
CPC classification number: C12N15/1034 , C12N15/1062
Abstract: This invention relates generally to systems and methods for identifying and selecting, desired proteins or nucleic acid molecules by linking mRNA, with known or unknown sequences, to its translated protein to form a cognate pair. The cognate pair is selected based upon desired properties of the protein or the nucleic acid. This method also includes the evolution of a desired protein or nucleic acid molecule by amplifying the nucleic acid portion of the selected cognate pair, introducing variation into the nucleic acid, translating the nucleic acid, attaching the nucleic acid to its protein to form a second cognate pair, and re-selecting this cognate pair based upon desired properties. Modified MRNAs operable to crosslink to tRNAs are also provided. Methods of producing a psoralen monoadduct or a crosslink are also provided.
Abstract translation: 本发明一般涉及通过将mRNA与已知或未知序列连接到其翻译的蛋白质以形成同源对来鉴定和选择所需蛋白质或核酸分子的系统和方法。 基于蛋白质或核酸的所需性质选择同源对。 该方法还包括通过扩增所选择的同源对的核酸部分，引入核酸的变异，翻译核酸，将核酸连接到其蛋白质以形成第二个同源物，从而演化期望的蛋白质或核酸分子 并且基于所需的属性重新选择该同源对。 还提供了可操作地与tRNA交联的修饰MRNA。 还提供了补骨脂素单加成物或交联剂的制备方法。
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公开(公告)号：US20030100004A1
公开(公告)日：2003-05-29
申请号：US10302456
申请日：2002-11-21
Inventor： Markus Kurz
IPC: C12Q001/68 , G01N033/53 , C12M001/34 , C12P021/06
CPC classification number: C12N15/1062 , C07K1/047 , C07K17/06
Abstract: Disclosed herein are methods for immobilizing a peptide or protein on a solid support. The method generally includes the following steps: (a) providing one or more templates attached to a solid support, wherein the one or more templates include (i) an RNA encoding a peptide and (ii) a peptide acceptor-linker linked to the RNA; and (b) subjecting the one or more templates to conditions that support translation and attachment of said peptide to said peptide acceptor, thereby synthesizing the one or more peptides on the solid support. Also disclosed herein are solid supports having at least one RNA-protein fusion component immobilized thereon, methods for generating protein arrays, and methods for screening molecules using these arrays.
Abstract translation: 本文公开了将肽或蛋白质固定在固体支持物上的方法。 该方法通常包括以下步骤：（a）提供连接到固体支持物上的一个或多个模板，其中所述一个或多个模板包括（i）编码肽的RNA和（ii）与RNA连接的肽受体 - 连接体 ; 和（b）使所述一个或多个模板经受支持所述肽转移和附着到所述肽受体的条件，由此合成固体支持物上的一种或多种肽。 本文还公开了其上固定有至少一种RNA-蛋白质融合组分的固体支持物，用于产生蛋白质阵列的方法，以及使用这些阵列筛选分子的方法。
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公开(公告)号：US20030082768A1
公开(公告)日：2003-05-01
申请号：US10177257
申请日：2002-06-20
Applicant: Whitehead Institute for Biomedical Research
Inventor： Donald Scott Baskerville , David P. Bartel
IPC: C12Q001/68 , C07H021/02 , C12N009/00
CPC classification number: C12N15/113 , C07K2319/00 , C12N15/1062 , C12N15/1075 , C12N2310/12 , C12N2310/3513
Abstract: Engineered mRNA useful in producing libraries of engineered mRNAs, polypeptide-engineered mRNA conjugates and diverse encoded polypeptide libraries, as well as novel ribozyes that join an mRNA to the translation product of the mRNA and methods of identifying members of diverse encoded polypeptide libraries which exhibit a desired activity. Also described are polypeptide-nucleic acid tag conjugates, methods of producing the conjugates and uses therefor.
Abstract translation: 可用于产生工程化mRNA文库，多肽工程改造的mRNA缀合物和多种已编码多肽文库的工程化mRNA，以及将mRNA加入mRNA翻译产物的新型核糖核酸酶，以及识别不同编码多肽文库成员的方法， 期望的活动。 还描述了多肽 - 核酸标签缀合物，产生缀合物的方法及其用途。
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