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    Leporipox-based vector vaccines
    21.
    发明授权
    Leporipox-based vector vaccines 失效
    基于Leporipox的载体疫苗

    公开(公告)号：US06942864B2

    公开(公告)日：2005-09-13

    申请号：US10471300

    申请日：2002-03-07

    Applicant: Norman Spibey

    Inventor： Norman Spibey

    IPC: C12N15/09 ,  A61K39/00 ,  A61K39/12 ,  A61K39/125 ,  A61K39/21 ,  A61K39/23 ,  A61K39/275 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N15/863 ,  A61K39/295 ,  C12N7/01

    CPC classification number: A61K39/275 ,  A61K39/12 ,  A61K39/125 ,  A61K39/21 ,  A61K39/23 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/552 ,  C12N15/86 ,  C12N2710/24034 ,  C12N2710/24043 ,  C12N2740/13034 ,  C12N2750/14034 ,  C12N2770/16034

    Abstract: The present invention is directed to the use of a live, recombinant leporipox virus comprising exogenous DNA, which is operably linked to at least one expression control element and which is incorporated in a non-essential region of the virus genome, in the manufacture of a vector vaccine for the treatment and/or prophylaxis of infectious diseases in non-lepori species. The invention furthermore relates to a live, recombinant leporipox virus comprising exogenous DNA operably linked to at least one expression control element and incorporated in a non-essential region of the virus genome characterized in that said exogenous DNA encodes at least one antigen of a non-lepori pathogen. Due to its restricted host-range the recombinant leporipox virus is non-pathogenic in non-susceptible hosts such as non-lepori vertebrates. Vaccination with said recombinant leporipox virus induced an antigen or immunogenic response in the vaccinated non-lepori host even though productive replication of the virus was not observed in the host.

    Abstract translation: 本发明涉及包含外源DNA的活的重组leporipox病毒的用途，所述外源DNA可操作地连接至至少一种表达控制元件，并且其结合到病毒基因组的非必需区域中，用于制备 用于治疗和/或预防非球孢属物种中的传染病的载体疫苗。 本发明还涉及一种活的重组leporipox病毒，其包含与至少一种表达控制元件可操作地连接并并入病毒基因组的非必需区域的外源DNA，其特征在于，所述外源DNA编码至少一种非 - 细菌病原体 由于其宿主范围受限，重组leporipox病毒在非敏感宿主如非细菌脊椎动物中是非致病性的。 使用所述重组leporipox病毒接种疫苗在非接种疫苗的宿主中诱导抗原或免疫原性反应，尽管在宿主中没有观察到病毒的生产性复制。

    Cell cultures
    22.
    发明授权
    Cell cultures 失效
    细胞培养

    公开(公告)号：US4021302A

    公开(公告)日：1977-05-03

    申请号：US606025

    申请日：1975-08-20

    Applicant: Sidney Edwin Smith ,  Kevin Joseph O'Reilly ,  John Prydie

    Inventor： Sidney Edwin Smith ,  Kevin Joseph O'Reilly ,  John Prydie

    IPC: A61K39/15 ,  A61K39/23 ,  A61K39/265 ,  C12N7/00 ,  C12N7/08 ,  C12K9/00

    CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/23 ,  A61K39/265 ,  A61K2039/5254 ,  C12N2710/16734 ,  C12N2750/14034 ,  C12N2750/14064 ,  C12N2750/14334 ,  C12N2750/14364

    Abstract: Cell culture comprising a novel cell strain in suitable nutrient medium, wherein the novel cell strain is produced by disaggregated feline embryo tissue cell and serially passaging the same from about the 20th to about the 48th passage level, so as to be cultured and subcultured with the resultant cell strains remaining substantially diploid, typically fibroblastic and substantially free from morphological transformations. Subculturing is facilitated by treatment of a confluent sheet of the disaggregated cells chelating agent before each transfer into a new batch of nutrient medium.

    Abstract translation: 细胞培养物在合适的营养培养基中包含新的细胞株，其中新细胞株由分解的猫胚胎组织细胞产生，并从大约20至大约第48个传代水平连续地传代，以便培养和传代培养 所得细胞株基本上保留二倍体，通常成纤维细胞并且基本上没有形态转化。 通过在每次转移到新的营养培养基中之前处理分解的细胞螯合剂的汇合片来促进亚培养。

    PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS
    28.
    发明申请
    PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS 有权
    PORCINE TORQUE TENO VIRUS疫苗和诊断

    公开(公告)号：US20160216263A1

    公开(公告)日：2016-07-28

    申请号：US14946384

    申请日：2015-11-19

    Applicant: Virginia Tech Intellectual Properties, Inc.

    Inventor： Xiang-Jin MENG ,  Yaowei Huang

    IPC: G01N33/569

    CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/552 ,  C07K14/005 ,  C07K16/081 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/14034 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2333/085 ,  G01N2469/20

    Abstract: The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.

    Abstract translation: 本发明提供了含有编码猪扭转腱病毒（PTTV）基因型或亚型PTTV1a-VA，PTTV1b-VA，PTTV2b-VA和PTTV2c-VA的多核酸分子的四种纯化制剂。 本发明还提供含有感染性核酸基因组分子的感染性DNA克隆，生物功能质粒或病毒载体。 本发明进一步提供活的，减毒的载体表达和纯化的重组衣壳亚单位或灭活的病毒疫苗以防止PTTV感染。 本发明另外提供了包含PTTV特异性基因产物的亚单位疫苗，特别是用于保护免受PTTV感染的ORF1衣壳基因产物。 此外，本发明提供了使用PTTV1，PTTV2和个体PTTV1基因型的特异性引物通过聚合酶链反应（PCR）诊断PTTV感染的方法。 最后，本发明提供了通过免疫学方法诊断PTTV感染的方法，例如使用PTTV特异性抗原检测血清PTTV特异性抗体的酶联免疫吸附测定（ELISA）和蛋白质印迹。

    Raccoon poxvirus expressing rabies glycoproteins
    29.
    发明授权
    Raccoon poxvirus expressing rabies glycoproteins 有权
    表达狂犬病糖蛋白的浣熊痘病毒

    公开(公告)号：US08795681B2

    公开(公告)日：2014-08-05

    申请号：US12128296

    申请日：2008-05-28

    Applicant: Stephen Qitu Wu ,  Michael A. Gill ,  Hsien-Jue Chu

    Inventor： Stephen Qitu Wu ,  Michael A. Gill ,  Hsien-Jue Chu

    IPC: A61K39/205 ,  A61K39/275 ,  A61K39/295 ,  A61K39/116 ,  C12N7/01 ,  C12N15/863

    CPC classification number: A61K39/205 ,  A61K39/0225 ,  A61K39/12 ,  A61K2039/521 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/552 ,  A61K2039/55566 ,  A61K2039/70 ,  C12N15/86 ,  C12N2710/10334 ,  C12N2710/24143 ,  C12N2750/14034 ,  C12N2760/18434 ,  C12N2760/18734 ,  C12N2760/20134 ,  C12N2770/20034

    Abstract: The present invention relates to recombinant raccoon poxvirus vectors that express the rabies virus glycoprotein gene at the hemagglutinin (ha) locus of the poxvirus genome or express the glycoprotein gene of the same or different rabies strains at the thymidine kinase (tk) and the hemagglutinin (ha) loci of the poxvirus genome, and their use as adjuvant-free vaccines. The raccoon poxvirus vector comprises the nucleic acid molecules encoding the glycoprotein of a Challenge Virus Standard rabies strain inserted and expressed at the tk locus of the poxvirus genome and of a Pasteur-Paris rabies strain inserted and expressed at the ha locus of the poxvirus genome. The vaccine may optionally contain a mixture of additional feline and canine antigens for immunization of animals. Also disclosed are methods for inducing an immune response to rabies in a mammal by administering to the mammal an effective immunizing amount of the vaccine of the invention.

    Abstract translation: 本发明涉及在痘病毒基因组的血凝素（ha）位点处表达狂犬病病毒糖蛋白基因的重组浣熊痘病毒载体，或表达胸苷激酶（tk）和血凝素（tk）上相同或不同狂犬病毒株的糖蛋白基因 ha）痘病毒基因组的基因座，以及它们作为无佐剂疫苗的用途。 浣熊痘病毒载体包含编码在痘病毒基因组的tk基因座插入和表达的挑战病毒标准狂犬病毒株的糖蛋白的核酸分子和在痘病毒基因组的ha位点插入和表达的巴斯德 - 巴黎狂犬病毒株。 疫苗可以任选地含有用于免疫动物的另外的猫科动物和犬抗原的混合物。 还公开了通过向哺乳动物施用有效免疫量的本发明的疫苗来诱导对哺乳动物的狂犬病免疫应答的方法。

    PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS
    30.
    发明申请
    PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS 有权
    PORCINE TORQUE TENO VIRUS疫苗和诊断

    公开(公告)号：US20110045019A1

    公开(公告)日：2011-02-24

    申请号：US12861378

    申请日：2010-08-23

    Applicant: XIANG-JIN MENG ,  YAOWEI HUANG

    Inventor： XIANG-JIN MENG ,  YAOWEI HUANG

    IPC: A61K39/12 ,  C12N15/33 ,  C12N15/70 ,  C12N1/21 ,  C12N7/00 ,  C12Q1/70 ,  G01N33/53

    CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/552 ,  C07K14/005 ,  C07K16/081 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/14034 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2333/085 ,  G01N2469/20

    Abstract: The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.

    Abstract translation: 本发明提供了含有编码猪扭转腱病毒（PTTV）基因型或亚型PTTV1a-VA，PTTV1b-VA，PTTV2b-VA和PTTV2c-VA的多核酸分子的四种纯化制剂。 本发明还提供含有感染性核酸基因组分子的感染性DNA克隆，生物功能质粒或病毒载体。 本发明进一步提供活的，减毒的载体表达和纯化的重组衣壳亚单位或灭活的病毒疫苗以防止PTTV感染。 本发明另外提供了包含PTTV特异性基因产物的亚单位疫苗，特别是用于保护免受PTTV感染的ORF1衣壳基因产物。 此外，本发明提供了使用PTTV1，PTTV2和个体PTTV1基因型的特异性引物通过聚合酶链反应（PCR）诊断PTTV感染的方法。 最后，本发明提供了通过免疫学方法诊断PTTV感染的方法，例如使用PTTV特异性抗原检测血清PTTV特异性抗体的酶联免疫吸附测定（ELISA）和蛋白质印迹。

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