公开(公告)号：US20160243149A1

公开(公告)日：2016-08-25

申请号：US15073037

申请日：2016-03-17

申请人： The Regents of the University of California

发明人： Bert L. Semler ,  Richard Virgen-Slane ,  Janet M. Rozovics ,  Paul D. Gershon

IPC分类号： A61K31/7076 ,  C12N9/16 ,  G01N27/447 ,  C12Q1/44

CPC分类号： A61K31/7076 ,  A61K31/00 ,  C07H19/20 ,  C12N9/16 ,  C12N9/22 ,  C12Q1/44 ,  C12Q1/70 ,  C12Y301/04 ,  G01N27/4476 ,  G01N27/44791 ,  G01N33/5008 ,  G01N2333/085 ,  G01N2333/916 ,  Y02A50/465

摘要： Anti-viral therapeutics for use in viral targets having VPg unlinkase activity, including the broad class of picornaviruses, and therapeutic methods directed at suppression of such activity are provided. Such anti-viral therapies for use against human rhinovirus, for example, are particularly desirable as they would lessen both the severity and duration of upper respiratory distress in both normal and asthmatic individuals. Assays and purification protocols for detecting and purifying VPg unlinkase are also provided.

公开(公告)号：US09402892B2

公开(公告)日：2016-08-02

申请号：US13977399

申请日：2011-12-29

申请人： Andrew MacAdam

发明人： Andrew MacAdam

IPC分类号： C07K14/105 ,  C07K14/085 ,  A61K39/13 ,  A61K39/125 ,  C12N15/43 ,  C12N15/41 ,  C12N7/00 ,  A61K39/12 ,  A61K39/00

CPC分类号： A61K39/13 ,  A61K39/12 ,  A61K39/125 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N2770/32011 ,  C12N2770/32611 ,  C12N2770/32621 ,  C12N2770/32634 ,  C12N2770/32662 ,  G01N2333/085 ,  Y02A50/466

摘要： The invention provides an attenuated polio virus having a 5′ non-coding region consisting of the 5′ non-coding region of Sabin 3, modified so that it does not have a base pair mismatch in stem (a) or (b) of domain V, wherein seven or eight of the base pairs in stems (a) and (b) are U-A or A-U base pairs; and a capsid protein from the Sabin 1, Mahoney, MEF or Saukett strain.

摘要翻译： 本发明提供了具有由Sabin 3的5'非编码区组成的5'非编码区的减毒脊髓灰质炎病毒，所述5'非编码区被修饰，使得其在结构域的茎（a）或（b）中不具有碱基对失配 V，其中茎（a）和（b）中的七个或八个碱基对是UA或AU碱基对; 和Sabin 1，Mahoney，MEF或Saukett菌株的衣壳蛋白。

公开(公告)号：US09175073B2

公开(公告)日：2015-11-03

申请号：US13840927

申请日：2013-03-15

申请人： Eisai R&D Management Co., Ltd.

发明人： Keiko Yamaguchi ,  Toshio Imai ,  Kenzo Muramoto

IPC分类号： C07K16/00 ,  C07K16/46 ,  C07K16/24 ,  C07K16/18 ,  C07K16/28 ,  G01N33/569

CPC分类号： C07K16/18 ,  C07K16/2803 ,  C07K2317/24 ,  C07K2317/569 ,  C07K2317/76 ,  G01N33/56972 ,  G01N2333/075 ,  G01N2333/085

摘要： The inventors discovered that the adhesion molecule CAR, known to be localized in intracellular adhesion sites, functioned as an adhesion molecule for activated lymphocytes. Further, the inventors identified CARL, a novel CAR ligand expressed in lymphocytes, and clarified that the ligand was expressed selectively in Th1 cells. In addition, they found that anti-CAR antibodies could inhibit the adhesion of activated lymphocytes to CAR molecules. Thus, the present invention provides methods for detecting Th1 cells using CAR or anti-CARL antibodies, and methods of screening for inhibitors suppressing the adhesion of Th1 cells using the binding between CAR and CARL as an index. Furthermore, the present invention relates to methods of screening for inhibitors of the binding between CAR and CARL, antibodies that inhibit the binding between CAR and CARL, and therapeutic compositions comprising these antibodies. These are expected to be useful in diagnosing diseases, such as inflammation, in which infiltration of Th1 cells is involved, and in providing pharmaceutical agents for alleviating such diseases.

摘要翻译： 本发明人发现已知局限在细胞内粘附位点的粘附分子CAR用作活化淋巴细胞的粘附分子。 此外，本发明人鉴定了CARL，一种在淋巴细胞中表达的新型CAR配体，并阐明了配体在Th1细胞中有选择性表达。 此外，他们发现抗CAR抗体可以抑制活化淋巴细胞对CAR分子的粘附。 因此，本发明提供了使用CAR或抗CARL抗体检测Th1细胞的方法，以及使用CAR和CARL之间的结合作为指标筛选抑制Th1细胞粘附的抑制剂的方法。 此外，本发明涉及筛选CAR和CARL之间的结合抑制剂的方法，抑制CAR和CARL之间的结合的抗体以及包含这些抗体的治疗组合物。 预期这些可用于诊断涉及Th1细胞浸润的疾病，例如炎症，以及提供减轻这些疾病的药物。

公开(公告)号：US20140348798A1

公开(公告)日：2014-11-27

申请号：US14201640

申请日：2014-03-07

申请人： NEOTROPIX, INC.

发明人： Paul L. HALLENBECK ,  Seshidhar Reddy POLICE ,  Laura M. HALES ,  Carl M. HAY ,  Shanthi GANESH ,  Ling XU ,  Jingping YANG ,  Cheng CHENG

IPC分类号： A61K35/76 ,  G01N33/50 ,  C07K16/10 ,  C07K14/005 ,  C12N7/00

CPC分类号： A61K35/768 ,  A61K39/00 ,  A61K2039/525 ,  C07K14/005 ,  C07K16/1009 ,  C12N7/00 ,  C12N2770/32021 ,  C12N2770/32022 ,  C12N2770/32032 ,  C12N2770/32033 ,  C12N2770/32061 ,  G01N33/5011 ,  G01N2333/085

摘要： The present invention relates to a novel RNA picornavirus that is called Seneca Valley virus (“SVV”). The invention provides isolated SVV nucleic acids and proteins encoded by these nucleic acids. Further, the invention provides antibodies that are raised against the SVV proteins. Because SVV has the ability to selectively kill some types of tumors, the invention provides methods of using SVV and SVV polypeptides to treat cancer. Because SVV specifically targets certain tumors, the invention provides methods of using SVV nucleic acids and proteins to detect cancer. Additionally, due to the information provided by the tumor-specific mechanisms of SVV, the invention provides methods of making new oncolytic virus derivatives and of altering viruses to have tumor-specific tropisms.

摘要翻译： 本发明涉及一种称为塞尼卡谷病毒（“SVV”）的新型小RNA病毒RNA。 本发明提供了由这些核酸编码的分离的SVV核酸和蛋白质。 此外，本发明提供针对SVV蛋白产生的抗体。 由于SVV具有选择性地杀死一些类型的肿瘤的能力，本发明提供了使用SVV和SVV多肽治疗癌症的方法。 由于SVV特异性靶向某些肿瘤，本发明提供了使用SVV核酸和蛋白质来检测癌症的方法。 另外，由于SVV的肿瘤特异性机制提供的信息，本发明提供了制备新的溶瘤病毒衍生物和改变病毒以具有肿瘤特异性嗜性的方法。

公开(公告)号：US08753622B2

公开(公告)日：2014-06-17

申请号：US13209124

申请日：2011-08-12

申请人： Paul Hallenbeck ,  Seshidhar Reddy Police ,  Laura M. Hales ,  Carl Hay ,  Shanthi Ganesh ,  Ling Xu ,  Jingping Yang ,  Cheng Cheng

发明人： Paul Hallenbeck ,  Seshidhar Reddy Police ,  Laura M. Hales ,  Carl Hay ,  Shanthi Ganesh ,  Ling Xu ,  Jingping Yang ,  Cheng Cheng

IPC分类号： A61K35/76 ,  C12N7/00 ,  C07K14/005 ,  A61K39/00

CPC分类号： A61K35/768 ,  A61K39/00 ,  A61K2039/525 ,  C07K14/005 ,  C07K16/1009 ,  C12N7/00 ,  C12N2770/32021 ,  C12N2770/32022 ,  C12N2770/32032 ,  C12N2770/32033 ,  C12N2770/32061 ,  G01N33/5011 ,  G01N2333/085

摘要： The present invention relates to a novel RNA picornavirus that is called Seneca Valley virus (“SVV”). The invention provides isolated SVV nucleic acids and proteins encoded by these nucleic acids. Further, the invention provides antibodies that are raised against the SVV proteins. Because SVV has the ability to selectively kill some types of tumors, the invention provides methods of using SVV and SVV polypeptides to treat cancer. Because SVV specifically targets certain tumors, the invention provides methods of using SVV nucleic acids and proteins to detect cancer. Additionally, due to the information provided by the tumor-specific mechanisms of SVV, the invention provides methods of making new oncolytic virus derivatives and of altering viruses to have tumor-specific tropisms.

摘要翻译： 本发明涉及一种称为塞尼卡谷病毒（“SVV”）的新型小RNA病毒RNA。 本发明提供了由这些核酸编码的分离的SVV核酸和蛋白质。 此外，本发明提供针对SVV蛋白产生的抗体。 由于SVV具有选择性地杀死一些类型的肿瘤的能力，本发明提供了使用SVV和SVV多肽治疗癌症的方法。 由于SVV特异性靶向某些肿瘤，本发明提供了使用SVV核酸和蛋白质来检测癌症的方法。 另外，由于SVV的肿瘤特异性机制提供的信息，本发明提供了制备新的溶瘤病毒衍生物和改变病毒以具有肿瘤特异性嗜性的方法。

公开(公告)号：US20140024015A1

公开(公告)日：2014-01-23

申请号：US13787982

申请日：2013-03-07

申请人： IDEXX Laboratories, Inc.

发明人： Lela Kay Riley ,  Judith D. Gohndrone ,  Matthew Howard Myles

IPC分类号： C12Q1/70

CPC分类号： C12Q1/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/32221 ,  C12N2770/32222 ,  C12Q1/701 ,  G01N2333/085 ,  G01N2469/20

摘要： The invention provides an isolated Boone cardiovirus, Boone cardiovirus polypeptides, polynucleotides and antibodies specific for Boone cardiovirus polypeptides. Also provided are methods for detection of Boone cardiovirus.

摘要翻译： 本发明提供了分离的Boone心脏病毒，Boone心脏病毒多肽，多核苷酸和对Boone心脏病毒多肽特异的抗体。 还提供了用于检测Boone心脏病毒的方法。

公开(公告)号：US20130344108A1

公开(公告)日：2013-12-26

申请号：US13977399

申请日：2011-12-29

申请人： Andrew MacAdam

发明人： Andrew MacAdam

IPC分类号： C12N7/00

CPC分类号： A61K39/13 ,  A61K39/12 ,  A61K39/125 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/5256 ,  C12N7/00 ,  C12N2770/32011 ,  C12N2770/32611 ,  C12N2770/32621 ,  C12N2770/32634 ,  C12N2770/32662 ,  G01N2333/085 ,  Y02A50/466

摘要： The invention provides an attenuated polio virus having a 5′ non-coding region consisting of the 5′ non-coding region of Sabin 3, modified so that it does not have a base pair mismatch in stem (a) or (b) of domain V, wherein seven or eight of the base pairs in stems (a) and (b) are U-A or A-U base pairs; and a capsid protein from the Sabin 1, Mahoney, MEF or Saukett strain.

摘要翻译： 本发明提供了具有由Sabin 3的5'非编码区组成的5'非编码区的减毒脊髓灰质炎病毒，所述5'非编码区被修饰，使得其在结构域的茎（a）或（b）中不具有碱基对失配 V，其中茎（a）和（b）中的七个或八个碱基对是UA或AU碱基对; 和Sabin 1，Mahoney，MEF或Saukett菌株的衣壳蛋白。

公开(公告)号：US20130230523A1

公开(公告)日：2013-09-05

申请号：US13840927

申请日：2013-03-15

申请人： EISAI R&D MANAGEMENT CO., LTD.

发明人： Keiko Yamaguchi ,  Toshio Imai ,  Kenzo Muramoto

IPC分类号： C07K16/28

CPC分类号： C07K16/18 ,  C07K16/2803 ,  C07K2317/24 ,  C07K2317/569 ,  C07K2317/76 ,  G01N33/56972 ,  G01N2333/075 ,  G01N2333/085

摘要： The inventors discovered that the adhesion molecule CAR, known to be localized in intracellular adhesion sites, functioned as an adhesion molecule for activated lymphocytes. Further, the inventors identified CARL, a novel CAR ligand expressed in lymphocytes, and clarified that the ligand was expressed selectively in Th1 cells. In addition, they found that anti-CAR antibodies could inhibit the adhesion of activated lymphocytes to CAR molecules. Thus, the present invention provides methods for detecting Th1 cells using CAR or anti-CARL antibodies, and methods of screening for inhibitors suppressing the adhesion of Th1 cells using the binding between CAR and CARL as an index. Furthermore, the present invention relates to methods of screening for inhibitors of the binding between CAR and CARL, antibodies that inhibit the binding between CAR and CARL, and therapeutic compositions comprising these antibodies. These are expected to be useful in diagnosing diseases, such as inflammation, in which infiltration of Th1 cells is involved, and in providing pharmaceutical agents for alleviating such diseases.

公开(公告)号：US07815913B2

公开(公告)日：2010-10-19

申请号：US12211523

申请日：2008-09-16

申请人： Hema Masarapu ,  Singanallur Balasubramanian Nagendrakumar ,  Dorairajan Thiagarajan ,  Villuppanoor Alwar Srinivasan

发明人： Hema Masarapu ,  Singanallur Balasubramanian Nagendrakumar ,  Dorairajan Thiagarajan ,  Villuppanoor Alwar Srinivasan

IPC分类号： A61K39/12 ,  A61K39/00 ,  A61K39/135

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N2750/14322 ,  C12N2770/32122 ,  C12N2770/40022 ,  C12N2770/40023 ,  G01N2333/08 ,  G01N2333/085 ,  G01N2469/20

摘要： The present disclosure relates to chimeric tymovirus-like particles (TVLPs) comprising a fusion protein that further comprises of a first protein that is a truncated tymovirus coat protein and a second protein. These chimeric TVLPs are useful as antigens. The present disclosure provides a highly efficient means for differentiating Foot and Mouth Disease Virus (FMDV) infected animals from vaccinated animals. The present disclosure further provides a process for the production of chimeric TVLPs and a diagnostic kit for the determination of specific antibodies of FMDV to differentiate FMDV infected from vaccinated animals. The present disclosure also provides the use of the chimeric TVLPs for diagnostic purposes.

摘要翻译： 本公开涉及包含融合蛋白的嵌合型病毒样颗粒（TVLP），其还包含第一种蛋白质，其是截短的胸膜病毒外壳蛋白质和第二种蛋白质。 这些嵌合的TVLP可用作抗原。 本公开提供了用于区分接种疫苗的动物的口蹄疫病毒（FMDV）感染动物的高效手段。 本公开还提供了用于产生嵌合型TVLP的方法和用于测定FMDV的特异性抗体以区分被接种疫苗的动物感染的FMDV的诊断试剂盒。 本公开还提供了用于诊断目的的嵌合型TVLP的用途。

公开(公告)号：US4952493A

公开(公告)日：1990-08-28

申请号：US37963

申请日：1987-04-13

申请人： Charles A. Kettner ,  Bruce D. Korant

发明人： Charles A. Kettner ,  Bruce D. Korant

IPC分类号： G01N33/50 ,  A61K38/00 ,  C07K5/00 ,  C07K5/10 ,  C07K5/107 ,  C07K14/00 ,  C12P21/00 ,  C12Q1/37 ,  C12Q1/70

CPC分类号： C12Q1/37 ,  C07K5/00 ,  C07K5/1016 ,  C12Q1/70 ,  C12Q2337/12 ,  C12Q2337/22 ,  G01N2333/085 ,  Y10S530/802 ,  Y10S530/807 ,  Y10S530/826

摘要： A method for preparing selected peptide substrates for detecting the activity of virus-specified proteases is provided. Specific tetrapeptide substrates are disclosed which are conjugates of protease-cleavable indicator groups and peptide sequences resembling picornavirus protease cleavage recognition sites.

摘要翻译： 提供了用于制备用于检测病毒特异性蛋白酶活性的选定肽底物的方法。 公开了特异性四肽底物，其为蛋白酶切割指示剂基团和类似于小核糖核酸酶切割识别位点的肽序列的缀合物。