公开(公告)号：US12146189B2

公开(公告)日：2024-11-19

申请号：US16433380

申请日：2019-06-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Earl Hubbell

IPC: C40B50/16 ,  C12Q1/6869 ,  C12Q1/6874 ,  C40B20/04 ,  C40B70/00 ,  G16B30/00 ,  G16C20/64 ,  G16C99/00

Abstract: A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.

公开(公告)号：US20240376434A1

公开(公告)日：2024-11-14

申请号：US18690262

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  NUNC A/S

Inventor： Debra JOHNSON ,  Sune SPORRING

IPC: C12N5/0775 ,  A61K35/28 ,  C12N9/22

Abstract: The instant technology generally relates to methods and compositions for expansion of mesenchymal stem cells in culture on a modified surface in the absence of a cell feeder layer and the absence of a cell adhesive coating on the surface. In some instances, the mesenchymal stem cells are expanded on the modified surface in a serum-free culture medium.

公开(公告)号：US20240376418A1

公开(公告)日：2024-11-14

申请号：US18780337

申请日：2024-07-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nephi D. Jones ,  Christopher D. Brau

IPC: C12M1/06 ,  B01F27/1111 ,  B01F27/191 ,  B01F27/2111 ,  B01F35/41 ,  B01F35/513 ,  B01F101/44 ,  C12M1/00 ,  C12M1/02 ,  C12N1/00

Abstract: A fluid mixing system includes a collapsible container bounding a compartment and extending between a first end and an opposing second end. An elongated continuous drive line is at least partially disposed within the compartment of the container. A first portion of the drive line is laterally spaced apart from a second portion of the drive line, and the first portion of the drive line and the second portion of the drive line are rotatable within the compartment of the container.

公开(公告)号：US20240361348A1

公开(公告)日：2024-10-31

申请号：US18640345

申请日：2024-04-19

Applicant: Life Technologies Corporation

Inventor： Alexandre VLASSOV ,  Timothy BARTA ,  Justin INSLEE ,  Ethan GENTERT ,  Robert SMITH ,  Sara THIELE ,  Carolyn ADAMS ,  Kraig KRUGER ,  Robert Schneider ,  Wade LUKIANOW ,  Jay GRECO ,  Jason JONES ,  David CERRONE ,  Sumonto GHOSH ,  Brian VAJDA ,  Andrew BASTA ,  Allan EVANS ,  Christopher CROWLEY ,  Robert SETTERQUIST ,  Anne COX ,  Mark MAYERNICK

IPC: G01N35/10 ,  B01D61/58 ,  B01D69/02 ,  G01N1/40 ,  G01N21/59 ,  G01N35/00

CPC classification number: G01N35/1016 ,  B01D61/58 ,  B01D69/02 ,  G01N1/405 ,  G01N1/4077 ,  G01N21/59 ,  G01N35/00584 ,  G01N35/1081 ,  B01D2311/2642 ,  B01D2313/44 ,  B01D2313/50 ,  B01D2313/58 ,  B01D2319/025 ,  B01D2325/42 ,  G01N2001/4088 ,  G01N2035/1025 ,  G01N2035/1053

Abstract: Purifying target biomolecules, such as nucleic acids or proteins, from a biological source is a time intensive process and is typically performed by a skilled technician or scientist owing to the highly technical nature of the work. Systems, devices, and methods disclosed herein enable the automated bioprocessing and purification of target biomolecules from a biological source. For example, an instrument and disposable cartridge are provided for automatedly isolating and purifying nucleic acids (such as plasmid DNA from a bacterial culture) or for isolating protein from any biological sample. Such an exemplary instrument and cartridge can work in concert to timely release, mix, and move the target biomolecule and various reagents and buffers through a target biomolecule purification process, resulting in a purified target biomolecule with less manual oversight than traditional approaches.

公开(公告)号：US12131399B2

公开(公告)日：2024-10-29

申请号：US16543475

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shanti Shankar ,  Sean Zimmerman ,  Mark Beauchemin ,  John Thompson

IPC: G16B45/00 ,  G01N1/28 ,  G06T1/00 ,  G16B30/10 ,  G01N35/00

CPC classification number: G06T1/0014 ,  G01N2035/00801

Abstract: A method for user guided initiating of an instrument includes receiving a run plan via a user interface of the instrument; indicating on the user interface, based on the run plan, a consumable to be provided to the instrument; detecting the presence of the consumable using a vision system; and indicating the presence of the consumable via the user interface.

公开(公告)号：US12098424B2

公开(公告)日：2024-09-24

申请号：US16999001

申请日：2020-08-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marcin Sikora

IPC: C12Q1/6874 ,  G16B30/00 ,  G16B30/10

CPC classification number: C12Q1/6874 ,  G16B30/00 ,  G16B30/10 ,  C12Q1/6874 ,  C12Q2537/157 ,  C12Q2545/101

Abstract: A method for designing test or control sequences may include identifying, using a variant caller, loci with systematic errors present in a plurality of sequencing runs included in a training set of sequencing runs obtained using sequencing-by-synthesis; and selecting a representative set of loci, including selecting from the identified loci an approximately equal number of loci involving errors in A, T, C, and G homopolymers and selecting from the identified loci an approximately equal number of loci involving homopolymers having a length of two, three, and four.

公开(公告)号：US20240309441A1

公开(公告)日：2024-09-19

申请号：US18583747

申请日：2024-02-21

Applicant: Life Technologies Corporation

Inventor： Mingsong Chen ,  Soo Yong Lau ,  Kuan Moon Boo ,  Mauro Aguanno ,  Huei Yeo ,  Tiong Han Toh ,  Wei Fuh Teo

IPC: C12Q1/686 ,  B01L7/00 ,  G01N21/64 ,  G02B21/24

CPC classification number: C12Q1/686 ,  B01L7/52 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6458 ,  G02B21/244 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/168 ,  B01L2300/18 ,  G01N2021/6463

Abstract: An instrument for biological analysis includes a base, an excitation source, an optical sensor, an excitation optical system, and an emission optical system. The base is configured to receive a sample holder comprising a plurality of biological samples. The optical sensor is configured to receive emissions from the biological samples in response to the excitation source. The instrument may additionally include a sensor lens enclosed by a lens case and a focusing mechanism including a gear that engages the lens case, the focusing mechanism being accessible outside the enclosure for adjusting a focus. The instrument may further include a sensor aperture dispose along an emission optical path and a blocking structure disposed to cooperate with the sensor aperture such that none of the reflected radiation from an illuminated surface near the sample holder is received by the optical sensor that does not also reflect off another surface of the instrument.

公开(公告)号：US12092605B2

公开(公告)日：2024-09-17

申请号：US18166745

申请日：2023-02-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marc Haberstroh ,  Geoffrey Dahlhoff

IPC: G01N27/447 ,  G01F23/263 ,  G01R19/25

CPC classification number: G01N27/44713 ,  G01F23/263 ,  G01N27/44782 ,  G01R19/25

Abstract: The present disclosure relates, in some embodiments, to a system for measuring capillary electrophoresis current. The system includes a plurality of capillaries, where each capillary has a cathode end and an anode end. The system further includes a plurality of cathode buffers. Each of the cathode buffers is configured to be electrically isolated from the other cathode buffers. Further, each cathode buffer is associated with one capillary of the plurality of capillaries. The cathode end of each capillary is immersed in its associated cathode buffer. The system includes a plurality of current sensors, each current sensor associated with one capillary of the plurality of capillaries for measuring current. In some embodiments, the plurality of capillaries is four capillaries.

公开(公告)号：US12077742B2

公开(公告)日：2024-09-03

申请号：US16853865

申请日：2020-04-21

Applicant: Life Technologies Corporation

Inventor： Nephi D. Jones ,  Christopher D. Brau

IPC: C12M1/06 ,  B01F27/1111 ,  B01F27/191 ,  B01F27/2111 ,  B01F35/41 ,  B01F35/513 ,  B01F101/44 ,  C12M1/00 ,  C12M1/02 ,  C12N1/00

CPC classification number: C12M27/02 ,  B01F27/1111 ,  B01F27/191 ,  B01F27/2111 ,  B01F35/4121 ,  B01F35/513 ,  C12M1/007 ,  C12M1/02 ,  C12M23/14 ,  C12N1/00 ,  B01F2101/44

Abstract: A method for mixing a biological suspension includes disposing a biological suspension within a compartment of a container, the biological suspension comprising cells or microorganisms suspended within a nutrient growth medium; and rotating a first drive line and laterally spaced apart second drive line within the compartment of the container so as to cause the drive lines to twist into a helical configuration and mix the biological suspension.

公开(公告)号：US20240287428A1

公开(公告)日：2024-08-29

申请号：US18654917

申请日：2024-05-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael E. Goodwin ,  Nephi D. JONES

IPC: C12M1/00 ,  B01F27/07 ,  B01F27/113 ,  B01F27/2121 ,  B01F27/213 ,  B01F27/231 ,  B01F27/61 ,  B01F33/00 ,  B01F35/22 ,  B01F35/513 ,  B01F35/71 ,  C12M1/06 ,  C12M1/34 ,  C12M3/00

CPC classification number: C12M23/14 ,  B01F27/071 ,  B01F27/113 ,  B01F27/2121 ,  B01F27/213 ,  B01F27/2312 ,  B01F27/61 ,  B01F33/86 ,  B01F35/2209 ,  B01F35/513 ,  C12M23/26 ,  C12M23/48 ,  C12M23/52 ,  C12M27/02 ,  C12M27/06 ,  C12M41/00 ,  B01F35/715

Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.