公开(公告)号：US12006536B2

公开(公告)日：2024-06-11

申请号：US17399449

申请日：2021-08-11

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Linda Lee ,  Sam Woo ,  Peter Ma

IPC分类号： C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874

CPC分类号： C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

摘要： A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

公开(公告)号：US20240182988A1

公开(公告)日：2024-06-06

申请号：US18331811

申请日：2023-06-08

申请人： Montana State University

发明人： Blake A. WIEDENHEFT ,  Andrew SANTIAGO-FRANGOS ,  Anna A. NEMUDRAIA ,  Artem A. NEMUDRYI

IPC分类号： C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6853

CPC分类号： C12Q1/701 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6853

摘要： The disclosure relates to engineered systems and methods for detecting target nucleic acid in a sample, which may be a complex mixture. The systems and methods may improve sensitivity of target nucleic acid detection by enhancing signal generation. For example, signal generation may be enhanced through programmable capture and concentration of the target nucleic acid using an engineered type III CRISPR complex. Various ancillary nucleases such as Can1, Can2, and NucC are identified and may be used for detection. For example, binding of the engineered type III CRISPR complex may produce products that activate the identified ancillary nucleases. Different activators trigger changes in the substrate specificity of these nucleases. The activated nucleases may be used to detect programmatic detection of the target nucleic in the sample. The systems and methods are shown to detect viral RNA directly from nasopharyngeal swab samples.

公开(公告)号：US11993813B2

公开(公告)日：2024-05-28

申请号：US16643161

申请日：2018-07-06

申请人： MGI TECH CO., LTD.

发明人： Erkai Liu ,  Wenwei Zhang ,  Ao Chen ,  Chongjun Xu

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/6874 ,  C12Q1/6855

CPC分类号： C12Q1/6874 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q2521/307 ,  C12Q2525/197 ,  C12Q2531/137 ,  C12Q2537/157 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/197 ,  C12Q2531/137 ,  C12Q2537/157

摘要： A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.

公开(公告)号：US20240167104A1

公开(公告)日：2024-05-23

申请号：US18505697

申请日：2023-11-09

申请人： NIPPN CORPORATION ,  FASMAC CO., LTD.

发明人： Mika TSUNA ,  Yoshitaka HARADA ,  Yasuhiro SETO ,  Kazuto TAKASAKI

IPC分类号： C12Q1/689 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N33/52

CPC分类号： C12Q1/689 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N33/52 ,  C12Q2600/156 ,  G01N2458/00 ,  G01N2469/00

摘要： Novel means that enables detection of the monocytogenes bacterium alone distinctly from other bacteria belonging to the genus Listeria with sufficiently high accuracy is disclosed. The present inventors intensively analyzed the genome of the monocytogenes bacterium to identify two genes (the lmo0084 gene and the lmo2736 gene) as target regions with which the monocytogenes bacterium can be specifically detected distinctly from other bacteria belonging to the genus Listeria utilizing a nucleic acid amplification method. By a further intensive study of the base sequences of these two genes, primer setting regions for highly accurate, specific detection of the monocytogenes bacterium alone were identified, and preferred particular examples of PCR primer sets, LAMP primer sets, and real-time PCR primer-probe sets were established.

公开(公告)号：US11970733B2

公开(公告)日：2024-04-30

申请号：US16671979

申请日：2019-11-01

申请人： Synthego Corporation

发明人： Timothy Hsiau ,  Richard Stoner ,  Travis Maures ,  David Conant

IPC分类号： C12Q1/6869 ,  B01L3/00 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6806 ,  C12Q1/6853 ,  G01N33/487 ,  G06F17/18

CPC分类号： C12Q1/6869 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6806 ,  C12Q1/6853 ,  G01N33/48721 ,  G06F17/18 ,  B01L3/502761 ,  B01L2200/10 ,  C12N2310/20 ,  C12Q2563/116

摘要： The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.

公开(公告)号：US20240132534A1

公开(公告)日：2024-04-25

申请号：US18520609

申请日：2023-11-28

申请人： JIANGSU SYNTHGENE BIOTECHNOLOGY CO., LTD.

发明人： Jiaying MIAO ,  Lei HUANG ,  Qi SHEN

IPC分类号： C07H21/02 ,  C12Q1/6853

CPC分类号： C07H21/02 ,  C12Q1/6853

摘要： Provided is an oligonucleotide primer with an acyclic nucleoside structure for initial capping, which has a molecular structural formula of m7UNGpppA2′omepG, and has higher mRNA in vitro transcription efficiency, higher capping efficiency, lower immunogenicity and higher protein translation efficiency.

公开(公告)号：US20240117427A1

公开(公告)日：2024-04-11

申请号：US18527896

申请日：2023-12-04

申请人： President and Fellows of Harvard College

发明人： Xiaoliang Sunney Xie ,  Katsuyuki Shiroguchi ,  Peter A. Sims ,  Tony Z. Jia

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  C12Q1/6853

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6853 ,  C12N2320/10

摘要： Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

公开(公告)号：US11946101B2

公开(公告)日：2024-04-02

申请号：US17845169

申请日：2022-06-21

申请人： Natera, Inc.

发明人： Huseyin Eser Kirkizlar ,  Raheleh Salari ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann ,  Allison Ryan ,  Naresh Vankayalapati

IPC分类号： C07H21/04 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/00 ,  G16B20/10 ,  G16H10/40

CPC分类号： C12Q1/6858 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/00 ,  G16B20/10 ,  G16H10/40 ,  C12Q2539/10 ,  C12Q2600/106 ,  C12Q2600/156 ,  Y02A90/10

摘要： The invention provides improved methods, compositions, and kits for detecting ploidy of chromosome regions, e.g. for detecting cancer or a chromosomal abnormality in a gestating fetus. The methods can utilize a set of more than 200 SNPs that are found within haploblocks and can include analyzing a series of target chromosomal regions related to cancer or a chromosomal abnormality in a gestating fetus. Finally the method may use knowledge about chromosome crossover locations or a best fit algorithm for the analysis. The compositions may comprise more than 200 primers located within haplotype blocks known to show CNV.

公开(公告)号：US20240102086A1

公开(公告)日：2024-03-28

申请号：US18370566

申请日：2023-09-20

申请人： Bio-Rad Laboratories, Inc.

发明人： Séverine MARGERIDON ,  Monica HERRERA ,  Richard DANNEBAUM ,  Nyaradzo DZVOVA ,  Raymond-John ABAYAN ,  Darren R. LINK

IPC分类号： C12Q1/6853

CPC分类号： C12Q1/6853

摘要： Methods and compositions comprising primers and probes to detect nucleic acids are provided. The probes comprise a ribonucleotide that can be cleaved by an RNase H2 enzyme when the probe is annealed to a reverse complement of a universal sequence that is introduced to a target nucleic acid, for example via amplification.

公开(公告)号：US20240068015A1

公开(公告)日：2024-02-29

申请号：US18347910

申请日：2023-07-06

申请人： Triad National Security, LLC

发明人： Hong Cai ,  Jian Song

IPC分类号： C12Q1/6816 ,  C12Q1/6853 ,  C12Q1/70

CPC分类号： C12Q1/6816 ,  C12Q1/6853 ,  C12Q1/701 ,  C12Q2600/158 ,  C12Q2600/16

摘要： The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.