公开(公告)号：US20210299670A1

公开(公告)日：2021-09-30

申请号：US17211594

申请日：2021-03-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. LINK

IPC: B01L7/00 ,  C12Q1/686 ,  B01L3/02 ,  G01N21/64

Abstract: Methods and systems for thermally controlling a chemical reaction in droplets. In an exemplary method, a first thermal zone and a second thermal zone having different temperatures from one another may be created in a reaction chamber. An emulsion including droplets encapsulated by a carrier fluid may be held in the reaction chamber. The droplets may have a density mismatch with the carrier fluid, and each droplet may include one or more reactants for the chemical reaction. An orientation of the reaction chamber may be changed to move the droplets from the first thermal zone to the second thermal zone, such that a rate of the chemical reaction changes in at least a subset of the droplets.

公开(公告)号：US20240012017A1

公开(公告)日：2024-01-11

申请号：US18347549

申请日：2023-07-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steve HOBBS ,  Darren R. LINK ,  Stuart YOUNG ,  Andrew WALGRAVE ,  Carolyn REIFSNYDER ,  Jonathan C. FEARNOW ,  Douglas GREINER ,  Chris GERGLEY

IPC: G01N35/08 ,  B01D17/02

CPC classification number: G01N35/08 ,  B01D17/0214

Abstract: Systems, including methods and apparatus, for analyzing partitioned samples, such as droplets, using recirculated fluid. The systems may be used to analyze a plurality of partitioned samples, with fluid used with initial samples reused with later samples. This reuse, or recirculation, may reduce the amount of fluid required for performing multiple analyses, with concomitant reductions in costs. Moreover, in some embodiments, it may simplify operation by increasing the number of analyses that may be performed before fluid must be replenished or replaced.

公开(公告)号：US20240102086A1

公开(公告)日：2024-03-28

申请号：US18370566

申请日：2023-09-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Séverine MARGERIDON ,  Monica HERRERA ,  Richard DANNEBAUM ,  Nyaradzo DZVOVA ,  Raymond-John ABAYAN ,  Darren R. LINK

IPC: C12Q1/6853

CPC classification number: C12Q1/6853

Abstract: Methods and compositions comprising primers and probes to detect nucleic acids are provided. The probes comprise a ribonucleotide that can be cleaved by an RNase H2 enzyme when the probe is annealed to a reverse complement of a universal sequence that is introduced to a target nucleic acid, for example via amplification.

公开(公告)号：US20240082847A1

公开(公告)日：2024-03-14

申请号：US18518124

申请日：2023-11-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. LINK

IPC: B01L7/00 ,  B01L3/02 ,  C12Q1/686 ,  G01N21/64

CPC classification number: B01L7/5255 ,  B01L3/0241 ,  C12Q1/686 ,  G01N21/6428 ,  B01L2300/08 ,  B01L2300/18 ,  B01L2400/021 ,  B01L2400/0409 ,  G01N2021/6432 ,  G01N2021/6439

Abstract: Methods and systems for thermally controlling a chemical reaction in droplets. In an exemplary method, a first thermal zone and a second thermal zone having different temperatures from one another may be created in a reaction chamber. An emulsion including droplets encapsulated by a carrier fluid may be held in the reaction chamber. The droplets may have a density mismatch with the carrier fluid, and each droplet may include one or more reactants for the chemical reaction. An orientation of the reaction chamber may be changed to move the droplets from the first thermal zone to the second thermal zone, such that a rate of the chemical reaction changes in at least a subset of the droplets.

公开(公告)号：US20220251628A1

公开(公告)日：2022-08-11

申请号：US17679310

申请日：2022-02-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. SAMUELS ,  Jeffrey Charles OLSON ,  Andrew WATSON ,  Keith BROWN ,  Darren R. LINK

IPC: C12Q1/6806 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.