公开(公告)号：US20230392198A1

公开(公告)日：2023-12-07

申请号：US18324885

申请日：2023-05-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Congcong MA ,  Duc DO ,  Douglas GREINER

IPC: C12Q1/6844 ,  C12N9/12 ,  C12Q1/6876

CPC classification number: C12Q1/6844 ,  C12N9/1276 ,  C12Q1/6876

Abstract: Method and system for detecting reverse transcriptase activity of a sample by digital assay. In an exemplary method, a reaction mixture including the sample, an RNA polymer, and reagents for reverse transcription may be prepared. Complementary DNA (cDNA) may be synthesized in the reaction mixture using the RNA polymer as a template. An amount of the cDNA synthesized may be proportional to the reverse transcriptase activity of the sample. Partitions may be formed after synthesizing the cDNA. The partitions may contain copies of the cDNA at partial occupancy. Each partition may include a portion of the reaction mixture. A target representing the cDNA may be amplified in the partitions. Amplification data for the target may be collected from the partitions.

公开(公告)号：US20240012017A1

公开(公告)日：2024-01-11

申请号：US18347549

申请日：2023-07-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steve HOBBS ,  Darren R. LINK ,  Stuart YOUNG ,  Andrew WALGRAVE ,  Carolyn REIFSNYDER ,  Jonathan C. FEARNOW ,  Douglas GREINER ,  Chris GERGLEY

IPC: G01N35/08 ,  B01D17/02

CPC classification number: G01N35/08 ,  B01D17/0214

Abstract: Systems, including methods and apparatus, for analyzing partitioned samples, such as droplets, using recirculated fluid. The systems may be used to analyze a plurality of partitioned samples, with fluid used with initial samples reused with later samples. This reuse, or recirculation, may reduce the amount of fluid required for performing multiple analyses, with concomitant reductions in costs. Moreover, in some embodiments, it may simplify operation by increasing the number of analyses that may be performed before fluid must be replenished or replaced.

公开(公告)号：US20210147925A1

公开(公告)日：2021-05-20

申请号：US17098006

申请日：2020-11-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： George KARLIN-NEUMANN ,  Douglas GREINER

IPC: C12Q1/6851 ,  C12Q1/6827 ,  C12Q1/6806 ,  C12Q1/6886

Abstract: Methods of analyzing a sample including cells and/or cell-free nuclei. In an exemplary method, partitions may be formed, with each partition including a portion of the same sample. Each partition of at least a subset of the partitions may contain only one of the cells/nuclei from the sample. Cells and/or cell-free nuclei from the sample may be lysed in the partitions. At least one amplification reaction may be performed for a target or set of targets in the partitions. Amplification data may be collected from the partitions in an exponential/linear phase of each amplification reaction. A copy number of the target or set of targets may be determined for individual partitions using the amplification data, to determine if either a duplication or deletion is present in all or a subset of the cells analyzed.