公开(公告)号：US11946101B2

公开(公告)日：2024-04-02

申请号：US17845169

申请日：2022-06-21

申请人： Natera, Inc.

发明人： Huseyin Eser Kirkizlar ,  Raheleh Salari ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann ,  Allison Ryan ,  Naresh Vankayalapati

IPC分类号： C07H21/04 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/00 ,  G16B20/10 ,  G16H10/40

CPC分类号： C12Q1/6858 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6886 ,  G16B20/00 ,  G16B20/10 ,  G16H10/40 ,  C12Q2539/10 ,  C12Q2600/106 ,  C12Q2600/156 ,  Y02A90/10

摘要： The invention provides improved methods, compositions, and kits for detecting ploidy of chromosome regions, e.g. for detecting cancer or a chromosomal abnormality in a gestating fetus. The methods can utilize a set of more than 200 SNPs that are found within haploblocks and can include analyzing a series of target chromosomal regions related to cancer or a chromosomal abnormality in a gestating fetus. Finally the method may use knowledge about chromosome crossover locations or a best fit algorithm for the analysis. The compositions may comprise more than 200 primers located within haplotype blocks known to show CNV.

公开(公告)号：US12024738B2

公开(公告)日：2024-07-02

申请号：US16382520

申请日：2019-04-12

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Raheleh Salari ,  Ryan Swenerton ,  Hsin-Ta Wu ,  Himanshu Sethi

IPC分类号： C12Q1/6827 ,  C12Q1/6886

CPC分类号： C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： The invention provides methods for detecting single nucleotide variants in breast cancer, bladder cancer, or colorectal cancer. Additional methods and compositions, such as reaction mixtures and solid supports comprising clonal populations of nucleic acids, are provided. For example, provided here is a method for monitoring and detection of early relapse or metastasis of breast cancer, bladder cancer, or colorectal cancer, comprising generating a set of amplicons by performing a multiplex amplification reaction on nucleic acids isolated from a sample of blood or urine or a fraction thereof from a patient who has been treated for a breast cancer, bladder cancer, or colorectal cancer, wherein each amplicon of the set of amplicons spans at least one single nucleotide variant locus of a set of patient-specific single nucleotide variant loci associated with the breast cancer, bladder cancer, or colorectal cancer; and determining the sequence of at least a segment of each amplicon of the set of amplicons that comprises a patient-specific single nucleotide variant locus, wherein detection of one or more patient-specific single nucleotide variants is indicative of early relapse or metastasis of breast cancer, bladder cancer, or colorectal cancer.

公开(公告)号：US11479812B2

公开(公告)日：2022-10-25

申请号：US15573800

申请日：2016-05-10

申请人： Natera, Inc.

发明人： Huseyin Eser Kirkizlar ,  Raheleh Salari ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann ,  Allison Ryan ,  Naresh Vankayalapati

IPC分类号： C07H21/04 ,  C12Q1/6858 ,  G16B20/00 ,  C12Q1/6869 ,  G16B20/10 ,  G16H10/40 ,  C12Q1/6886

摘要： The invention provides improved methods, compositions, and kits for detecting ploidy of chromosome regions, e.g. for detecting cancer or a chromosomal abnormality in a gestating fetus. The methods can utilize a set of more than 200 SNPs that are found within haploblocks and can include analyzing a series of target chromosomal regions related to cancer or a chromosomal abnormality in a gestating fetus. Finally the method may use knowledge about chromosome crossover locations or a best fit algorithm for the analysis. The compositions may comprise more than 200 primers located within haplotype blocks known to show CNV.

公开(公告)号：US11447820B2

公开(公告)日：2022-09-20

申请号：US15573800

申请日：2016-05-10

申请人： Natera, Inc.

发明人： Huseyin Eser Kirkizlar ,  Raheleh Salari ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann ,  Allison Ryan ,  Naresh Vankayalapati

IPC分类号： C07H21/04 ,  C12Q1/6858 ,  G16B20/00 ,  C12Q1/6869 ,  G16B20/10 ,  G16H10/40 ,  C12Q1/6886

摘要： The invention provides improved methods, compositions, and kits for detecting ploidy of chromosome regions, e.g. for detecting cancer or a chromosomal abnormality in a gestating fetus. The methods can utilize a set of more than 200 SNPs that are found within haploblocks and can include analyzing a series of target chromosomal regions related to cancer or a chromosomal abnormality in a gestating fetus. Finally the method may use knowledge about chromosome crossover locations or a best fit algorithm for the analysis. The compositions may comprise more than 200 primers located within haplotype blocks known to show CNV.