公开(公告)号：US10533219B2

公开(公告)日：2020-01-14

申请号：US15716058

申请日：2017-09-26

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US11168362B2

公开(公告)日：2021-11-09

申请号：US16418104

申请日：2019-05-21

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US10577650B2

公开(公告)日：2020-03-03

申请号：US15716331

申请日：2017-09-26

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US10011870B2

公开(公告)日：2018-07-03

申请号：US15372279

申请日：2016-12-07

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US12024738B2

公开(公告)日：2024-07-02

申请号：US16382520

申请日：2019-04-12

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Raheleh Salari ,  Ryan Swenerton ,  Hsin-Ta Wu ,  Himanshu Sethi

IPC分类号： C12Q1/6827 ,  C12Q1/6886

CPC分类号： C12Q1/6827 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

摘要： The invention provides methods for detecting single nucleotide variants in breast cancer, bladder cancer, or colorectal cancer. Additional methods and compositions, such as reaction mixtures and solid supports comprising clonal populations of nucleic acids, are provided. For example, provided here is a method for monitoring and detection of early relapse or metastasis of breast cancer, bladder cancer, or colorectal cancer, comprising generating a set of amplicons by performing a multiplex amplification reaction on nucleic acids isolated from a sample of blood or urine or a fraction thereof from a patient who has been treated for a breast cancer, bladder cancer, or colorectal cancer, wherein each amplicon of the set of amplicons spans at least one single nucleotide variant locus of a set of patient-specific single nucleotide variant loci associated with the breast cancer, bladder cancer, or colorectal cancer; and determining the sequence of at least a segment of each amplicon of the set of amplicons that comprises a patient-specific single nucleotide variant locus, wherein detection of one or more patient-specific single nucleotide variants is indicative of early relapse or metastasis of breast cancer, bladder cancer, or colorectal cancer.

公开(公告)号：US11525159B2

公开(公告)日：2022-12-13

申请号：US17165592

申请日：2021-02-02

申请人： Natera, Inc.

发明人： Solomon Moshkevich ,  Bernhard Zimmermann ,  Tudor Pompiliu Constantin ,  Huseyin Eser Kirkizlar ,  Allison Ryan ,  Styrmir Sigurjonsson ,  Felipe Acosta Archila ,  Ryan Swenerton

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6851

摘要： The present disclosure provides methods for determining the status of an allograft within a transplant recipient from genotypic data measured from a mixed sample of DNA comprising DNA from both the transplant recipient and from the donor. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

公开(公告)号：US11530442B2

公开(公告)日：2022-12-20

申请号：US16418104

申请日：2019-05-21

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US11519028B2

公开(公告)日：2022-12-06

申请号：US17494726

申请日：2021-10-05

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/6806 ,  C12Q1/6869

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US11066703B2

公开(公告)日：2021-07-20

申请号：US16418104

申请日：2019-05-21

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.