公开(公告)号：US20130008795A1

公开(公告)日：2013-01-10

申请号：US13366816

申请日：2012-02-06

Applicant: Gyula VIGH ,  Peniel LIM

Inventor： Gyula VIGH ,  Peniel LIM

IPC: B01D61/46 ,  G01N27/453

CPC classification number: G01N27/44795 ,  B01D61/425 ,  C07K1/28 ,  G01N27/44773

Abstract: An electrophoresis apparatus for measuring, characterizing and/or altering a composition of a sample. The apparatus comprises an anode compartment having an anode and a cathode compartment having a cathode. The anode and cathode are spaced at a distance from one another to define an electric field having a direction along longitudinal axis, and between the anode and cathode compartments can be at least one separation compartment. Each compartment includes means for adding or removing a solution, a first dimension orthogonal to the direction of the electric field, a second dimension orthogonal to the electric field and the first dimension, and a third dimension parallel to the electric field and orthogonal to the first and second dimensions. A ratio of the first and second dimensions define an aspect ratio, at least one aspect ratio being less than one. An ion-permeable barrier is positioned between each compartment to prevent convective mixing therebetween.

Abstract translation: 一种用于测量，表征和/或改变样品组成的电泳装置。 该装置包括具有阳极和具有阴极的阴极室的阳极室。 阳极和阴极彼此间隔开一定距离以限定具有沿着纵向轴线的方向的电场，并且在阳极和阴极室之间可以是至少一个分隔室。 每个隔室包括用于添加或去除溶液的装置，与电场方向正交的第一维度，与电场和第一维度正交的第二维度，以及平行于电场并与第一维度正交的第三维度 和第二维度。 第一和第二尺寸的比率定义纵横比，至少一个纵横比小于1。 离子渗透屏障位于每个隔室之间，以防止它们之间的对流混合。

公开(公告)号：US08282799B2

公开(公告)日：2012-10-09

申请号：US13180823

申请日：2011-07-12

Applicant: Lotien Richard Huang ,  James Christopher Sturm ,  Robert Hamilton Austin

Inventor： Lotien Richard Huang ,  James Christopher Sturm ,  Robert Hamilton Austin

IPC: G01N27/447 ,  G01N27/453 ,  G01N30/02 ,  G01N15/06

CPC classification number: B07B1/00 ,  B01L3/5027 ,  G01N27/44704 ,  G01N27/44773 ,  G01N27/44791 ,  G01N30/0005 ,  G01N30/02 ,  G01N30/6095 ,  B01D15/34

Abstract: The present invention relates to methods and devices for separating particles according to size. More specifically, the present invention relates to a microfluidic method and device for the separation of particles according to size using an array comprising a network of gaps, wherein the field flux from each gap divides unequally into subsequent gaps. In one embodiment, the array comprises an ordered array of obstacles in a microfluidic channel, in which the obstacle array is asymmetric with respect to the direction of an applied field.

Abstract translation: 本发明涉及根据尺寸分离颗粒的方法和装置。 更具体地说，本发明涉及一种用于使用包括间隙网络的阵列根据尺寸分离颗粒的微流体方法和装置，其中来自每个间隙的场磁通不均匀地分成随后的间隙。 在一个实施例中，阵列包括在微流体通道中的有序阵列的障碍物，其中障碍物阵列相对于施加场的方向是不对称的。

公开(公告)号：US20120138468A1

公开(公告)日：2012-06-07

申请号：US13390352

申请日：2010-08-18

Applicant: Uri Sivan ,  Elad Brod

Inventor： Uri Sivan ,  Elad Brod

IPC: B01D61/44 ,  B01D57/02

CPC classification number: G01N27/44795 ,  B01D57/02 ,  B01D61/445 ,  C07K1/28 ,  G01N21/80 ,  G01N27/44747 ,  G01N27/44773

Abstract: A device for isoelectric focusing. The device comprises a focusing container configured to contain an electrolyte solution and having a longitudinal axis and at least one electrolysis unit mounted in a close proximity to the longitudinal axis. Each electrolysis unit injects an ion flow into the focusing container so as to create a pH gradient having a plurality of steps in the electrolyte solution, along the longitudinal axis. Each step has a substantially uniform pH level and the pH gradient is defined by at least one pH ramp between every two sequential steps of the plurality of steps.

Abstract translation: 等电聚焦装置。 该装置包括配置成容纳电解质溶液并具有纵向轴线的至少一个电解单元的聚焦容器，并且至少一个电解单元安装在靠近纵向轴线的位置。 每个电解单元将离子流注入到聚焦容器中，以沿着纵向轴线产生在电解液中具有多个步骤的pH梯度。 每个步骤具有基本上均匀的pH水平，并且pH梯度由多个步骤的每两个连续步骤之间的至少一个pH斜坡限定。

公开(公告)号：US20120058467A1

公开(公告)日：2012-03-08

申请号：US12746169

申请日：2008-12-05

Applicant: Nicholas Thomas ,  Michael Kenrick ,  Simon Stubbs

Inventor： Nicholas Thomas ,  Michael Kenrick ,  Simon Stubbs

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: G01N27/3275 ,  B01L3/5085 ,  B01L2300/046 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2400/0421 ,  G01N27/44773

Abstract: The invention relates to an apparatus and an in-situ method for detecting cellular DNA damage. The invention is particularly suited for use in Comet assays and for automation of such assays. The apparatus comprises a multiwell plate, the plate comprising an array of wells held in fixed relationship with each other and each well having an axis, side walls, and a base and wherein the well is provided with electrode pairs disposed therein; and wherein there is provided means for parallel connection of the electrode pairs to an external voltage supply.

Abstract translation: 本发明涉及用于检测细胞DNA损伤的装置和原位方法。 本发明特别适用于彗星测定和这种测定的自动化。 该设备包括多孔板，该板包括彼此保持固定关系的孔阵列，每个孔具有轴线，侧壁和基底，并且其中设置有设置在其中的电极对; 并且其中提供了用于将电极对并联连接到外部电压源的装置。

公开(公告)号：US08031925B2

公开(公告)日：2011-10-04

申请号：US11971041

申请日：2008-01-08

Applicant: Jeffrey S. Morris ,  Howard Gutstein

Inventor： Jeffrey S. Morris ,  Howard Gutstein

IPC: G06K9/00 ,  G01N33/48

CPC classification number: G01N27/44721 ,  G01N27/44773 ,  G06T7/0012 ,  G06T2207/30072

Abstract: The present inventions is directed to a method and computer program product for detecting and quantifying protein spots, including: generating an average gel image by taking a pixel-by-pixel average of the intensities of a plurality of aligned gel images; detecting spots on the average gel image using pinnacle detection; and quantifying spots on individual gels using the maximum intensity within fixed neighborhoods surrounding pinnacle locations found in the average gel image.

Abstract translation: 本发明涉及一种用于检测和定量蛋白质斑点的方法和计算机程序产品，包括：通过采取多个对准凝胶图像的强度的逐像素平均值来产生平均凝胶图像; 使用峰值检测在平均凝胶图像上检测斑点; 并使用在平均凝胶图像中发现的围绕顶点位置的固定邻域内的最大强度来量化单个凝胶上的斑点。

公开(公告)号：US20110114485A1

公开(公告)日：2011-05-19

申请号：US13054271

申请日：2009-07-15

Applicant: Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

Inventor： Koji Sakairi ,  Satonari Akutsu ,  Yuji Maruo ,  Takateru Matsunaga ,  Yutaka Unuma ,  Atsunori Hiratsuka ,  Hideki Kinoshita ,  Nao Sakaguchi ,  Kenji Yokoyama

IPC: G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44773

Abstract: An electrophoresis apparatus includes a sample separating section for containing a sample separating medium for separating a sample in a horizontal direction, the sample separating section containing the sample separating medium in such a manner that the sample separating medium has an exposed portion at least one end of a surface of the sample separating medium, the surface being in parallel with the horizontal direction; and medium connecting means for connecting a sample containing medium to the sample separating medium at a connecting region, the sample containing medium containing a sample, and the connecting region satisfying a particular equation. By this, an electrophoresis technique with better accuracy than conventional arts can be provided.

Abstract translation: 电泳装置包括样品分离部分，用于容纳用于在水平方向上分离样品的样品分离介质，样品分离部分包含样品分离介质，使得样品分离介质具有暴露部分，至少一端 所述样品分离介质的表面与所述水平方向平行; 以及介质连接装置，用于在连接区域连接包含样品的介质到样品分离介质，所述样品包含含有样品的介质和满足特定等式的连接区域。 由此，可以提供比现有技术更好的精度的电泳技术。

公开(公告)号：USRE42249E1

公开(公告)日：2011-03-29

申请号：US12217114

申请日：2008-07-01

Applicant: Gabriel P. Lopez ,  Steven R. J. Brueck ,  Linnea K. Ista

Inventor： Gabriel P. Lopez ,  Steven R. J. Brueck ,  Linnea K. Ista

IPC: B01D21/00

CPC classification number: G01N27/44773 ,  B82Y30/00 ,  G01N30/00 ,  G01N30/88 ,  G01N2030/8813 ,  Y10S977/717 ,  Y10S977/845 ,  Y10T436/25375 ,  Y10T436/255

Abstract: The present invention provides a nonostructured device comprising a substrate including nanotroughs therein; and a lipid bilayer suspended on or supported in the substrate. A separation method is also provided comprising the steps of supporting or suspending a lipid bilayer on a substrate; wherein the subtrate comprises nanostructures and wherein the lipid bilayer comprises at least one membrane associated biomolecule; and applying a driving force to the lipid bilayer to separate the membrane associated biomolecule from the lipid bilayer and to drive the membrane associated biomolecule into the nanostructures. A fluidic device for separating particles according to size is provided including a fluidic channel, and a matrix comprising a plurality of protrusions within the fluidic channel, wherein the device provides a driving force to the particles being separated through the fluidic channel; and wherein a flow of the driving force from between the protrusions is divided unequally into a major flow component and a minor flow component, each component flowing between subsequent protrusions in the matrix, such that the average direction of the major flow component is not parallel to the average direction of the driving force, and, when particles are introduced into the matrix, particles having a size less than a predetermined critical size are transported generally in the average direction of the driving force, and particles having a size at least that of the critical size are transported generally in the average direction of the major flow component, thereby separating the particles according to size. Methods for separating particles including steps of separation based on size and affinity are also provided.

Abstract translation: 本发明提供一种非结构化装置，其包括其中包括纳入通道的基板; 和悬浮在或支撑在基底中的脂质双层。 还提供了一种分离方法，包括以下步骤：在基质上支持或悬浮脂质双层; 其中所述缓冲液包含纳米结构，并且其中所述脂质双层包含至少一个膜相关的生物分子; 并将驱动力施加到脂质双层以将膜相关生物分子与脂质双层分离并将膜相关联的生物分子驱动到纳米结构中。 提供了一种用于根据尺寸分离颗粒的流体装置，包括流体通道和在流体通道内包括多个突起的基体，其中该装置为通过流体通道分离的颗粒提供驱动力; 并且其中来自所述突起之间的驱动力的流动不均匀地分成主流动分量和小流动分量，每个分量在所述基体中的后续突起之间流动，使得主流动分量的平均方向不平行于 驱动力的平均方向，并且当颗粒被引入到基质中时，尺寸小于预定临界尺寸的颗粒通常沿驱动力的平均方向传送，并且尺寸至少大于 临界尺寸通常沿主流体组分的平均方向输送，从而根据尺寸分离颗粒。 还提供了基于尺寸和亲和力分离包括分离步骤的颗粒的方法。

公开(公告)号：US07687257B2

公开(公告)日：2010-03-30

申请号：US11386781

申请日：2006-03-23

Applicant: Nicolas Ugolin ,  Sylvie Chevillard ,  Catherine Ory ,  Jerome Lebeau

Inventor： Nicolas Ugolin ,  Sylvie Chevillard ,  Catherine Ory ,  Jerome Lebeau

IPC: C12M1/34

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  B01L2300/0819 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0478 ,  G01N1/405 ,  G01N27/44773 ,  G01N27/44782

Abstract: The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.

Abstract translation: 本发明涉及用于分离和/分析溶解在复合混合物中的几种分子靶的装置，其特征在于其包括a）微柱基质，其中每个微柱（2）包含固定的分子探针，用于保留 通过特异性探针/靶连接物包含在复合物混合物中的特定分子靶，b）用于将引入本发明装置的复合物混合物循环到a），（c）中定义的基质的每个微柱的第一毛细管网（3） 第二毛细管网络（4），用于在洗脱后使保留在微柱上的分子靶朝向传感器（5）循环，用于进行其回收和/或分析，以及d）如果需要，传感器（5） ，优选以质谱仪的形式，用于进行不同分子靶标的回收和/或分析。

公开(公告)号：US20090319450A1

公开(公告)日：2009-12-24

申请号：US12373675

申请日：2007-07-09

Applicant: Reiji Teramoto ,  Hirotaka Minagawa ,  Kenichi Kamijo

Inventor： Reiji Teramoto ,  Hirotaka Minagawa ,  Kenichi Kamijo

IPC: G06F15/18 ,  G06F19/00 ,  G06N7/02

CPC classification number: G16B40/00 ,  G01N27/44773 ,  G16B20/00

Abstract: A protein search method for searching for, as a target protein, a protein having direct or indirect relevance to information based on protein representation profiling data acquired by means of proteome analysis includes: determining, as a target protein, a protein that is relevant to the information based on significance of proteins obtained by using supervised learning from the information and the protein representation in the profiling data; and evaluating the performance of the target protein by means of evaluation data.

Abstract translation: 一种蛋白质搜索方法，用于搜索基于通过蛋白质组分析获得的蛋白质表达谱分析数据与信息直接或间接相关的蛋白质的蛋白质搜索方法，包括：将作为靶蛋白的蛋白质确定为与 基于通过使用来自信息的监督学习获得的蛋白质的信息和在分析数据中的蛋白质表示的信息; 并通过评估数据评估靶蛋白的表现。

公开(公告)号：US07514000B2

公开(公告)日：2009-04-07

申请号：US11479125

申请日：2006-06-30

Applicant: John R. Gilbert ,  Manish Deshpande ,  Jaishree Trikha

Inventor： John R. Gilbert ,  Manish Deshpande ,  Jaishree Trikha

IPC: B01D15/00 ,  B01D61/00 ,  B01D63/00

CPC classification number: B01D63/087 ,  B01D63/088 ,  B01D67/0034 ,  B01D67/0062 ,  B01D2313/13 ,  B01D2313/40 ,  B01D2325/028 ,  B01D2325/12 ,  B01L3/06 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/146 ,  B01L2300/041 ,  B01L2300/047 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2400/0421 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0666 ,  C02F2209/36 ,  C30B7/00 ,  C30B29/58 ,  G01N27/44743 ,  G01N27/44773 ,  Y10S977/786 ,  Y10T156/10

Abstract: A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. The microfluidic element may comprise a matrix having an affinity for selected molecules in a sample. The matrix binds, reacts with and/or retains the selected molecules without affecting other molecules in the sample.

Abstract translation: 用于在微流体系统中整合微流体组分的系统和方法使得微流体系统能够执行选定的微流体功能。 封盖模块包括用于执行微流控功能的微流体元件。 封盖模块堆叠在具有微流管道的微流体衬底上，以将微流控功能结合到系统中。 微流体元件可以包含对样品中选定分子具有亲和性的基质。 该基质与所选择的分子结合，反应和/或保留，而不影响样品中的其它分子。