公开(公告)号：US20050019819A1

公开(公告)日：2005-01-27

申请号：US10924151

申请日：2004-08-23

Applicant: Nigel Tooke ,  Philip Thomas ,  Michael Kenrick

Inventor： Nigel Tooke ,  Philip Thomas ,  Michael Kenrick

IPC: C12N15/09 ,  C12M1/00 ,  C12N5/06 ,  C12N15/10 ,  C12Q1/68 ,  C07H21/04 ,  C12M1/34

CPC classification number: C12N15/1003

Abstract: Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.

Abstract translation: 公开了一种用于从CD装置中的细胞样品分离DNA或细胞核或其混合物的方法和装置。 所述方法包括用裂解试剂处理全细胞的悬浮液，以便裂解细胞质膜和至少一些核膜，并将溶胞产物引入微加工装置的微通道中，其中每个微通道为 具有设置在通道中的屏障，以阻止DNA和细胞核的通过或流动，同时允许液体通过微通道，使得在通道中形成包含DNA的网。

公开(公告)号：US20120058467A1

公开(公告)日：2012-03-08

申请号：US12746169

申请日：2008-12-05

Applicant: Nicholas Thomas ,  Michael Kenrick ,  Simon Stubbs

Inventor： Nicholas Thomas ,  Michael Kenrick ,  Simon Stubbs

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: G01N27/3275 ,  B01L3/5085 ,  B01L2300/046 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2400/0421 ,  G01N27/44773

Abstract: The invention relates to an apparatus and an in-situ method for detecting cellular DNA damage. The invention is particularly suited for use in Comet assays and for automation of such assays. The apparatus comprises a multiwell plate, the plate comprising an array of wells held in fixed relationship with each other and each well having an axis, side walls, and a base and wherein the well is provided with electrode pairs disposed therein; and wherein there is provided means for parallel connection of the electrode pairs to an external voltage supply.

Abstract translation: 本发明涉及用于检测细胞DNA损伤的装置和原位方法。 本发明特别适用于彗星测定和这种测定的自动化。 该设备包括多孔板，该板包括彼此保持固定关系的孔阵列，每个孔具有轴线，侧壁和基底，并且其中设置有设置在其中的电极对; 并且其中提供了用于将电极对并联连接到外部电压源的装置。

公开(公告)号：US20070196822A1

公开(公告)日：2007-08-23

申请号：US11568574

申请日：2005-05-05

Applicant: Nicholas Thomas ,  Simon Stubbs ,  Rahman Ismail ,  Nigel Michael ,  Michael Kenrick ,  Johathan Kendall

Inventor： Nicholas Thomas ,  Simon Stubbs ,  Rahman Ismail ,  Nigel Michael ,  Michael Kenrick ,  Johathan Kendall

IPC: C12Q1/70 ,  G06F19/00 ,  C12N15/861

CPC classification number: G01N33/6803

Abstract: An in vitro method for characterising the activity and/or the function of a test agent on signalling pathways and/or cellular processes within a host cell is disclosed which is conducted on living cells in a none-destructive manner. The method of the invention may be used with an imaging system and a computerised data processing device.

Abstract translation: 公开了一种用于表征测试试剂在宿主细胞内的信号传导途径和/或细胞过程上的活性和/或功能的体外方法，其以无损的方式在活细胞上进行。 本发明的方法可以与成像系统和计算机化的数据处理装置一起使用。