公开(公告)号：US20180057814A1

公开(公告)日：2018-03-01

申请号：US15691721

申请日：2017-08-30

Applicant: Life Technologies Corporation

Inventor： Christopher BURNETT ,  Nitin Puri ,  Susan Magdaleno ,  Alexander Vlassov

IPC: C12N15/113 ,  A61K31/713 ,  C12N15/11

CPC classification number: C12N15/113 ,  A61K31/713 ,  A61K47/50 ,  C12N15/111 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/332 ,  C12N2310/3515 ,  C12N2310/3519 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/31

Abstract: The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.

公开(公告)号：US20180051281A1

公开(公告)日：2018-02-22

申请号：US15607295

申请日：2017-05-26

Applicant: Agilent Technologies, Inc.

Inventor： Daniel E. Ryan ,  Douglas J. Dellinger ,  Jeffrey R. Sampson ,  Robert Kaiser ,  Joel Myerson

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/113 ,  C12N2310/20

CPC classification number: C12N15/111 ,  C07H21/02 ,  C12N9/22 ,  C12N15/113 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/312 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/323 ,  C12N2310/3231 ,  C12N2310/333 ,  C12N2310/335 ,  C12N2310/346 ,  C12N2310/3517 ,  C12N2310/531 ,  C12N2320/51 ,  C12N2320/53 ,  C12N2330/31 ,  C12Q1/6876 ,  C12N2310/3521 ,  C12N2310/3533

Abstract: The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

公开(公告)号：US20180016585A1

公开(公告)日：2018-01-18

申请号：US15523829

申请日：2015-05-18

Applicant: Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

Inventor： Yong Wang ,  Zhiqiang Xiong ,  Shujie Song ,  Qiaoxia Liu ,  Jianfeng Wang

IPC: C12N15/70 ,  C12Q1/18 ,  C12N15/09 ,  C12R1/465 ,  C07H17/08

CPC classification number: C12N15/70 ,  C07H17/08 ,  C12N9/1288 ,  C12N15/09 ,  C12N15/113 ,  C12N2320/12 ,  C12N2330/31 ,  C12N2330/50 ,  C12P17/08 ,  C12P19/62 ,  C12Q1/18 ,  C12R1/465

Abstract: The present invention relates to a method for improving the heterologous synthesis of a polyketide by E. coli and use thereof. The yield of the polyketide heterologously synthesized by E. coli is significantly increased by attenuating the expression of seventy-two genes, such as sucC and talB, in a host strain, wherein the highest yield increase rate can reach 60% or more. Currently, erythromycin is the most clear model compound in the study on the biosynthesis of polyketids. The production strain of the present invention enables massive accumulation of 6-deoxyerythronolide (6-dEB), an erythromycin precursor, in the fermentation process, laying the foundation for the industrial production of the heterologous synthesis of erythromycin by E. coli.

公开(公告)号：US09850477B2

公开(公告)日：2017-12-26

申请号：US14936896

申请日：2015-11-10

Applicant: LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES

Inventor： Gerard Perret ,  Nicolas Bihoreau ,  Laurent Siret

IPC: C12N15/11 ,  C12N9/64 ,  C07K14/745 ,  C12N15/115

CPC classification number: C12N9/6432 ,  C07K14/745 ,  C12N9/6437 ,  C12N9/644 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/351 ,  C12N2330/31 ,  C12Y304/21006 ,  C12Y304/21021 ,  C12Y304/21022

Abstract: The invention relates to a method for purifying biologically active GLA-domain coagulation proteins, comprising the following steps: a) bringing a sample that contains one or more GLA-domain coagulation proteins and may contain biologically inactive molecules of GLA-domain protein(s), into contact with an affinity support on which nucleic aptamers that bind specifically to at least one biologically active GLA-domain coagulation protein are immobilized, in order to form complexes between (i) said nucleic aptamers and (ii) said GLA-domain coagulation protein(s), b) releasing the GLA-domain coagulation protein(s) from the complexes formed in step a), and c) recovering said biologically active GLA-domain coagulation protein(s) in a purified form.

公开(公告)号：US20170275620A1

公开(公告)日：2017-09-28

申请号：US15449444

申请日：2017-03-03

Applicant: Ionis Pharmaceuticals, Inc.

Inventor： Rosanne M. Crooke ,  Mark J. Graham ,  Kristina M. Lemonidis ,  Kenneth W. Dobie

IPC: C12N15/113 ,  A61K38/00

CPC classification number: C12N15/113 ,  A61K38/00 ,  C12N2310/11 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2310/351 ,  C12N2320/30 ,  C12N2330/31 ,  C12N2310/3521

Abstract: Compounds, compositions and methods are provided for modulating the expression of apolipoprotein C-III. The compositions comprise oligonucleotides, targeted to nucleic acid encoding apolipoprotein C-III. Methods of using these compounds for modulation of apolipoprotein C-III expression and for diagnosis and treatment of disease associated with expression of apolipoprotein C-III are provided

公开(公告)号：US20170081660A1

公开(公告)日：2017-03-23

申请号：US15268422

申请日：2016-09-16

Applicant: Twist Bioscience Corporation

Inventor： Anthony COX ,  Sebastian TREUSCH ,  Siyuan CHEN

IPC: C12N15/10 ,  C12N15/113 ,  C12Q1/68 ,  G01N33/50

CPC classification number: C12N15/1093 ,  C12N15/1079 ,  C12N15/113 ,  C12N2320/30 ,  C12N2330/31 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q2600/158 ,  C40B50/08 ,  C40B50/14 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/5041

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

公开(公告)号：US20170044546A1

公开(公告)日：2017-02-16

申请号：US15331833

申请日：2016-10-22

Applicant: Base Pair Biotechnologies, Inc.

Inventor： George W. Jackson

IPC: C12N15/115 ,  C12N15/10 ,  G01N33/53

CPC classification number: C12N15/115 ,  C12N15/1048 ,  C12N2310/16 ,  C12N2320/13 ,  C12N2330/31 ,  G01N33/5308 ,  G01N2333/70596 ,  G01N2333/765 ,  G01N2333/79

Abstract: The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules.

Abstract translation: 本发明涉及功能性配体与靶分子，特别是功能性核酸及其修饰，以及用于同时产生例如多种不同功能生物分子的方法，特别涉及同时产生多种不同功能性核酸的方法。 本发明还涉及与靶分子具有亲和力结合的功能性配体。

公开(公告)号：US20170022504A1

公开(公告)日：2017-01-26

申请号：US15171860

申请日：2016-06-02

Applicant: The General Hospital Corporation

Inventor： Jeannie T. Lee ,  Jing Zhao ,  Kavitha Sarma ,  Mark Borowsky ,  Toshiro Kendrick Ohsumi

IPC: C12N15/113 ,  A61K9/127 ,  A61K31/713

CPC classification number: C12N15/1137 ,  A61K9/1271 ,  A61K31/713 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/1136 ,  C12N2310/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2310/3233 ,  C12N2310/346 ,  C12N2320/30 ,  C12N2320/32 ,  C12N2330/31 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Q2600/178 ,  C12Y201/01043

Abstract: This invention relates to long non-coding RNAs (lncRNAs), libraries of those ncRNAs that bind chromatin modifiers, such as Polycomb Repressive Complex 2, inhibitory nucleic acids and methods and compositions for targeting lncRNAs.

Abstract translation: 本发明涉及长非编码RNA（lncRNA），结合染色质修饰剂的那些ncRNA的文库，例如Polycomb Repressive Complex 2，抑制性核酸以及用于靶向lncRNA的方法和组合物。

公开(公告)号：US20160340671A1

公开(公告)日：2016-11-24

申请号：US15174634

申请日：2016-06-06

Applicant: Cellecta, Inc.

Inventor： Alex Chenchik ,  Donato Tedesco ,  Mikhail Makhanov

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1082 ,  C12N15/1034 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/1079 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531 ,  C12N2330/31 ,  G01N33/5008 ,  C12Q2563/179

Abstract: Methods of clonal analysis of functional genomic assays are provided. Aspects of the invention include transducing a population of target cells with a packaged viral effector library made up of a plurality of effector construct subsets, wherein each effector construct subset of the library includes a plurality of effector constructs having a common effector cassette linked to a distinct clonal barcode. Inclusion of distinct clonal barcodes in the effector construct subset allows for determination of the clonal representation of an effector construct subset in transduced target cells that exhibit a specific phenotype. Aspects of the invention further include compositions, e.g., libraries and components thereof, which find use in practicing the methods.

Abstract translation: 提供功能基因组测定的克隆分析方法。 本发明的方面包括用由多个效应子构建子集构成的包装的病毒效应物库转导靶细胞群，其中文库的每个效应子构建子集包括多个效应构建体，其具有连接到不同 克隆条形码。 在效应子构建子集中包含不同的克隆条形码允许确定表现出特定表型的转导的靶细胞中效应子构建子集的克隆表达。 本发明的方面还包括可用于实施该方法的组合物，例如其文库及其组分。

公开(公告)号：US09493767B2

公开(公告)日：2016-11-15

申请号：US14406422

申请日：2013-06-19

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Gyoo Yeol Jung ,  Jina Yang ,  SungHo Jang ,  Sang Woo Seo

IPC: C12N15/11 ,  C12N15/10 ,  C12N15/115

CPC classification number: C12N15/1048 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/3519 ,  C12N2320/10 ,  C12N2320/13 ,  C12N2330/31

Abstract: A method of screening a high L-tryptophan-producing microorganism using a riboswitch is provided. More particularly, a riboswitch for screening a high L-tryptophan-producing microorganism including a tryptophan aptamer, a DNA sequence consisting of 1 to 20 nucleotides and a selectable marker gene, and a method of screening a high L-tryptophan-producing microorganism using the same are provided. The riboswitch and the method of screening a high L-tryptophan-producing microorganism using the same can be useful in selecting a strain producing a high concentration of L-tryptophan in a relatively quick and easy manner, and thus enhancing price competitiveness of tryptophan production using microorganisms.

Abstract translation: 提供了使用核糖开关筛选高L-色氨酸生产微生物的方法。 更具体地，涉及用于筛选含有色氨酸适体的高L-色氨酸生物微生物，由1至20个核苷酸组成的DNA序列和选择性标记基因的核糖开关以及使用该L-选择标记基因筛选高L-色氨酸的微生物的方法 同样提供。 核糖开关和使用其的高L-色氨酸生产微生物的筛选方法可用于以相对快速和容易的方式选择产生高浓度L-色氨酸的菌株，从而提高使用色氨酸生产的价格竞争力 微生物。