公开(公告)号：US10179899B2

公开(公告)日：2019-01-15

申请号：US14731459

申请日：2015-06-05

申请人： LUMINEX CORPORATION

发明人： Doug Whitman ,  James Heisler ,  Colin Bozarth ,  Germaine Siew ,  Charles Collins ,  Eric Smith

IPC分类号： B01L7/00 ,  C12M1/06 ,  B01F13/08

摘要： Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.

公开(公告)号：US20180318838A1

公开(公告)日：2018-11-08

申请号：US16032183

申请日：2018-07-11

申请人： LUMINEX CORPORATION

发明人： Dylan Hilmer BIRD ,  Jesus CHING ,  Bruce A. JOHNSON ,  Keith E. MORAVICK ,  Bruce RICHARDSON

IPC分类号： B01L7/00 ,  C12Q1/6806 ,  C12Q1/686 ,  B01F5/12 ,  B01F11/00 ,  B01L3/00 ,  B01F15/02 ,  G01N1/38 ,  G01N35/04 ,  G01N35/10

CPC分类号： B01L7/52 ,  B01F5/12 ,  B01F11/0071 ,  B01F15/0203 ,  B01F15/0237 ,  B01F2015/0221 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50851 ,  B01L3/523 ,  B01L3/527 ,  B01L7/00 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0683 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N1/38 ,  G01N35/1079 ,  G01N2035/0436 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/255

摘要： Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.

公开(公告)号：US09970050B2

公开(公告)日：2018-05-15

申请号：US14602846

申请日：2015-01-22

申请人： LUMINEX CORPORATION

发明人： Brian Schrader ,  Douglas F. Whitman

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6823 ,  C12Q1/6818

摘要： Methods and compositions for the detection and quantification of nucleic acids are provided. In one embodiment, a sample is contacted with a primer complementary to a first region of a target nucleic acid and a probe complementary to a second region of the target nucleic acid downstream of the first region under conditions suitable for hybridization of the target nucleic acid with the primer and the probe. The probe in this embodiment comprises a fluorophore and is attached to a solid support. The hybridized probe is cleaved with a nucleic acid polymerase having exonuclease activity to release the reporter from the solid support. The presence of the target nucleic acid is then detected and optionally quantified by detecting a decrease in signal from the reporter on the solid support.

公开(公告)号：US20170321259A1

公开(公告)日：2017-11-09

申请号：US15656541

申请日：2017-07-21

申请人： LUMINEX CORPORATION

发明人： Scott C. JOHNSON ,  Nicolas ARAB ,  Doug WHITMAN

IPC分类号： C12Q1/68

摘要： Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US20170321254A1

公开(公告)日：2017-11-09

申请号：US15654942

申请日：2017-07-20

申请人： LUMINEX CORPORATION

发明人： Scott JOHNSON ,  Jen DILLMAN

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/6848 ,  C12Q2549/101 ,  C12Q2563/137 ,  C12Q2563/149 ,  C12Q2563/159

摘要： Lyophilized biological reagents, such as enzymes (e.g., PCR reagents) and antibodies, are provided that include a wax component. Thus, in some aspects, a method is provided for storing a biological reagent comprising formulating the reagent into a lyophilized composition including a wax component. Methods for using such lyophilized reagents are likewise provided.

公开(公告)号：US09745438B2

公开(公告)日：2017-08-29

申请号：US14499343

申请日：2014-09-29

申请人： LUMINEX CORPORATION

发明人： Jason Bedre ,  Don Chandler ,  Ben Mize

IPC分类号： G01N21/76 ,  C08J9/26 ,  G01N33/543 ,  C09B67/20 ,  H01F1/00

CPC分类号： C08J9/26 ,  C08J2325/06 ,  C09B67/006 ,  G01N33/54313 ,  H01F1/00

摘要： Macroporous beads and a method of manufacturing and using such macroporous beads. wherein the beads are distinguishable for use in a multiplex assay. Preferably, the beads are distinguishable by two or more unique fluorochromes, and at least some of the beads are magnetically responsive. In a preferred form, some of the macroporous beads have interior pores with a different moiety from the exterior surface, allowing beads with different attached functional groups.

公开(公告)号：US20170080427A1

公开(公告)日：2017-03-23

申请号：US15366186

申请日：2016-12-01

申请人： LUMINEX CORPORATION

发明人： Dylan Hilmer BIRD ,  Jesus CHING ,  Bruce A. JOHNSON ,  Keith E. MORAVICK ,  Bruce RICHARDSON

IPC分类号： B01L7/00 ,  B01L3/00 ,  B01F5/12 ,  C12Q1/68

CPC分类号： B01L7/52 ,  B01F5/12 ,  B01F11/0071 ,  B01F15/0203 ,  B01F15/0237 ,  B01F2015/0221 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50851 ,  B01L3/523 ,  B01L3/527 ,  B01L7/00 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0683 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N1/38 ,  G01N35/1079 ,  G01N2035/0436 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/255

摘要： Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.

公开(公告)号：US09539577B2

公开(公告)日：2017-01-10

申请号：US14723586

申请日：2015-05-28

申请人： LUMINEX CORPORATION

发明人： Dylan Hilmer Bird ,  Jesus Ching ,  Bruce A. Johnson ,  Keith E. Moravick ,  Bruce Richardson

IPC分类号： C12M1/00 ,  B01L7/00 ,  B01F11/00 ,  B01F15/02 ,  B01L3/00 ,  G01N1/38 ,  C12Q1/68 ,  G01N35/10 ,  G01N35/04

CPC分类号： B01L7/52 ,  B01F5/12 ,  B01F11/0071 ,  B01F15/0203 ,  B01F15/0237 ,  B01F2015/0221 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50851 ,  B01L3/523 ,  B01L3/527 ,  B01L7/00 ,  B01L2200/025 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0622 ,  B01L2400/0633 ,  B01L2400/0644 ,  B01L2400/0683 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N1/38 ,  G01N35/1079 ,  G01N2035/0436 ,  Y10T436/118339 ,  Y10T436/25375 ,  Y10T436/255

摘要： Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.

摘要翻译： 还提供了用于分离生物样品和样品上的下游生物测定的柱，以及使用这种盒的方法。 在一个实施方案中，从生物样品中分离核酸样品，并且例如通过聚合酶链式反应扩增核酸样品。 本文提供的盒还可用于分离非核酸样品，例如蛋白质，并对蛋白质进行下游反应，例如结合测定。 还提供了用于进行下游生物测定和检测测定结果的仪器。

公开(公告)号：US09286504B2

公开(公告)日：2016-03-15

申请号：US14257630

申请日：2014-04-21

申请人： Luminex Corporation

发明人： Wayne D. Roth

IPC分类号： G06K9/00 ,  G01N15/14 ,  G06T7/00

CPC分类号： G06K9/0014 ,  G01N15/1463 ,  G06T7/0012 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30072

摘要： Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

公开(公告)号：US20160032373A1

公开(公告)日：2016-02-04

申请号：US14885351

申请日：2015-10-16

申请人： LUMINEX CORPORATION

发明人： Douglas F. WHITMAN ,  Charles J. COLLINS

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6834 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2561/113 ,  C12Q2563/143 ,  C12Q2537/143

摘要： The present invention provides methods and systems for real-time measurements of PCR with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the PCR process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the PCR process.