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    Nucleic acid amplification
    11.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US09309558B2

    公开(公告)日：2016-04-12

    申请号：US14085727

    申请日：2013-11-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Shiaw-Min Chen ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Nucleic acid amplification
    12.
    发明授权
    Nucleic acid amplification 有权

    公开(公告)号：US09309557B2

    公开(公告)日：2016-04-12

    申请号：US13842296

    申请日：2013-03-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chieh-Yuan Li ,  David Ruff ,  Shiaw-Min Chen ,  Jennifer O'Neil ,  Rachel Kasinskas ,  Jonathan Rothberg ,  Bin Li ,  Kai Qin Lao

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Apparatus and methods for performing electrochemical reactions
    14.
    发明授权
    Apparatus and methods for performing electrochemical reactions 有权

    公开(公告)号：US11567029B2

    公开(公告)日：2023-01-31

    申请号：US14162612

    申请日：2014-01-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John Nobile ,  George T. Roth ,  Todd Rearick ,  Jonathan M. Schultz ,  Jonathan Rothberg ,  David Marran

    IPC: G01N27/30 ,  G01N27/414 ,  B01L3/00

    Abstract: The invention is directed to apparatus and methods for delivering multiple reagents to, and monitoring, a plurality of analytical reactions carried out on a large-scale array of electronic sensors under minimal noise conditions. In one aspect, the invention provides method of improving signal-to-noise ratios of output signals from the electronic sensors sensing analytes or reaction byproducts by subtracting an average of output signals measured from neighboring sensors where analyte or reaction byproducts are absent. In other aspects, the invention provides an array of electronic sensors integrated with a microwell array for confining analytes and/or particles for analytical reactions and a method for identifying microwells containing analytes and/or particles by passing a sensor-active reagent over the array and correlating sensor response times to the presence or absence of analytes or particles. Such detection of analyte- or particle-containing microwells may be used as a step in additional noise reduction methods.

    Methods and apparatus for measuring analytes using large scale FET arrays
    15.
    发明授权
    Methods and apparatus for measuring analytes using large scale FET arrays 有权

    公开(公告)号：US11339430B2

    公开(公告)日：2022-05-24

    申请号：US16846113

    申请日：2020-04-10

    Applicant: Life Technologies Corporation

    Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

    IPC: C12Q1/6869 ,  H01L23/00 ,  H01L29/78 ,  G01N27/414 ,  C12Q1/6874 ,  H01L21/306 ,  H01L27/088

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

    Methods and apparatus for detecting molecular interactions using FET arrays
    17.
    发明授权
    Methods and apparatus for detecting molecular interactions using FET arrays 有权
    使用FET阵列检测分子相互作用的方法和装置

    公开(公告)号：US09039888B2

    公开(公告)日：2015-05-26

    申请号：US14033934

    申请日：2013-09-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Rothberg ,  Wolfgang Hinz

    IPC: G01N27/414 ,  G01N33/50 ,  G01N33/543 ,  C12Q1/68 ,  H01L29/78 ,  G01N27/26 ,  G01N33/68

    CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

    Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

    Abstract translation: 提供了与用于分析物检测和测量的大规模FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。

    Methods and Apparatus for Measuring Analytes Using Large Scale FET Arrays
    20.
    发明申请
    Methods and Apparatus for Measuring Analytes Using Large Scale FET Arrays 有权

    公开(公告)号：US20220340965A1

    公开(公告)日：2022-10-27

    申请号：US17738427

    申请日：2022-05-06

    Applicant: Life Technologies Corporation

    Inventor： Jonathan Rothberg ,  Wolfgang HINZ ,  Kim Johnson ,  James Bustillo

    IPC: C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  H01L21/306 ,  H01L23/00 ,  H01L27/088 ,  H01L29/78

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

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