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    CELL CARRIER, ASSOCIATED METHODS FOR MAKING CELL CARRIER AND CULTURING CELLS USING THE SAME
    13.
    发明申请
    CELL CARRIER, ASSOCIATED METHODS FOR MAKING CELL CARRIER AND CULTURING CELLS USING THE SAME 有权
    细胞载体，用于制备细胞载体和培养细胞的相关方法

    公开(公告)号：US20140356949A1

    公开(公告)日：2014-12-04

    申请号：US14461860

    申请日：2014-08-18

    Applicant: General Electric Company

    Inventor： Brian Michael Davis ,  Kenneth Roger Conway ,  Evelina Roxana Loghin ,  Andrew Arthur Paul Burns ,  David Gilles Gascoyne ,  Scott Michael Miller

    IPC: C12N5/074

    CPC classification number: C12N5/0696 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/00 ,  Y10T156/1039

    Abstract: A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.

    Abstract translation: 提供了用于诱导多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体的方法，以及使用相同的载体培养诱导的多能干细胞。

    SYSTEM FOR OPTIMIZING THE INTRODUCTION OF NUCLEIC ACIDS INTO CELLS USING MAGNETIC PARTICLES
    14.
    发明申请
    SYSTEM FOR OPTIMIZING THE INTRODUCTION OF NUCLEIC ACIDS INTO CELLS USING MAGNETIC PARTICLES 审中-公开
    使用磁性颗粒将核酸引入细胞的优化系统

    公开(公告)号：US20140093946A1

    公开(公告)日：2014-04-03

    申请号：US14039323

    申请日：2013-09-27

    Applicant: General Electric Company

    Inventor： Jean-Baptiste Mathieu ,  Brian Michael Davis ,  Vasile Bogdan Neculaes ,  Evelina Roxana Loghin ,  Kenneth Roger Conway

    IPC: C12N15/64

    CPC classification number: C12N15/64 ,  C12N15/87

    Abstract: An embodiment of a system is provided herein, wherein the system allows for the analysis and selection of numerous experimental conditions to optimize transfection efficiency and cell viability. The system is used for magnetic particle based nucleic acid delivery by optimizing various parameters. The system comprises a control module; an incubation module for incubating magnetic nanoparticle and nucleic acid; a transfection module and an analysis module.

    Abstract translation: 本文提供了一种系统的实施例，其中所述系统允许分析和选择许多实验条件以优化转染效率和细胞活力。 该系统通过优化各种参数用于基于磁性粒子的核酸递送。 该系统包括控制模块; 用于孵育磁性纳米颗粒和核酸的孵育模块; 转染模块和分析模块。

    SYSTEM AND METHOD FOR INITIATING A CELL CULTURE
    17.
    发明申请
    SYSTEM AND METHOD FOR INITIATING A CELL CULTURE 有权
    用于启动细胞培养的系统和方法

    公开(公告)号：US20170058252A1

    公开(公告)日：2017-03-02

    申请号：US14841072

    申请日：2015-08-31

    Applicant: General Electric Company

    Inventor： Chengkun Zhang ,  Reginald Donovan Smith ,  Kenneth Roger Conway ,  Anshika Bajaj

    IPC: C12M1/02 ,  C12M3/00 ,  C12M1/00 ,  C12Q3/00

    CPC classification number: C12M41/22 ,  C12M21/08 ,  C12M23/14 ,  C12M23/38 ,  C12M23/48 ,  C12M27/16 ,  C12M29/24 ,  C12M41/48 ,  C12Q3/00

    Abstract: System and methods are provided for initiating a cell culture. The systems and methods include a first enclosure containing a thermal mass positioned on a surface area of a platform. The systems and methods include a second enclosure, containing a culture fluid and a gas mixture, placed on the first enclosure such that the first enclosure is positioned between the second enclosure and the platform. The systems and methods further include a cover secured on the second enclosure, and generating first thermal energy on the surface area of the platform and second thermal energy from the cover.

    Abstract translation: 提供了用于启动细胞培养的系统和方法。 该系统和方法包括一个包含位于平台表面区域上的热质量的第一外壳。 所述系统和方法包括放置在第一外壳上的包含培养液和气体混合物的第二外壳，使得第一外壳位于第二外壳和平台之间。 所述系统和方法还包括固定在第二外壳上的盖，并且在平台的表面区域上产生第一热能并且从盖产生第二热能。

    SEMI-AUTOMATED SAMPLING SYSTEM FOR ASEPTIC SAMPLING
    20.
    发明申请
    SEMI-AUTOMATED SAMPLING SYSTEM FOR ASEPTIC SAMPLING 审中-公开
    用于自动采样的半自动采样系统

    公开(公告)号：US20160123848A1

    公开(公告)日：2016-05-05

    申请号：US14529201

    申请日：2014-10-31

    Applicant: General Electric Company

    Inventor： Weston Blaine Griffin ,  Chengkun Zhang ,  Kenneth Roger Conway ,  Reginald Donovan Smith

    IPC: G01N1/14 ,  G01N33/48 ,  G01N35/10

    CPC classification number: G01N1/14 ,  C12M29/12 ,  C12M33/06 ,  C12M37/02 ,  C12M41/00

    Abstract: A semi-automated sampling assembly configured for aseptic sampling at one or more instances from a sample source having a biological inoculum is provided. The semi-automated sampling assembly includes a sampling conduit, a recovery conduit, one or more sampling kits, and a pumping device. The sampling conduit includes a first port and a second port, where the first port of the sampling conduit is configured to be operatively coupled to the sample source. Further, the recovery conduit includes a first port and a second port, where the first port of the recovery conduit is configured to be operatively coupled to the sample source. Also, the second port of the recovery conduit is operatively coupled to at least a portion of the sampling conduit. Moreover, the one or more sampling kits are operatively coupled to the sampling conduit, and the pumping device is operatively coupled to the sampling conduit.

    Abstract translation: 提供了一种半自动采样组件，其被配置为在具有生物接种物的样品源的一个或多个实例处进行无菌取样。 半自动采样组件包括采样导管，回收管道，一个或多个采样套件以及泵送装置。 采样导管包括第一端口和第二端口，其中采样导管的第一端口被配置为可操作地耦合到采样源。 此外，回收管道包括第一端口和第二端口，其中回收管道的第一端口被配置为可操作地耦合到采样源。 此外，回收管道的第二端口可操作地联接到采样管道的至少一部分。 此外，一个或多个采样套件可操作地耦合到采样导管，并且泵送装置可操作地耦合到采样导管。

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