公开(公告)号：US20250091048A1

公开(公告)日：2025-03-20

申请号：US18888785

申请日：2024-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Serge Saxonov ,  Phillip Belgrader ,  Kevin D. Ness ,  Michael Y. Lucero ,  Billy W. Colston, JR. ,  Shawn Paul Hodges ,  Nicholas J. Heredia ,  Jeffrey Clark Mellen ,  Camille Bodley Troup ,  Paul Wyatt

IPC: B01L3/00 ,  B01F23/41 ,  B01F25/00 ,  B01F25/23 ,  B01F33/3011 ,  B01F33/302 ,  B01F33/81 ,  B01L9/00 ,  C12Q1/686 ,  G01N35/08

Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US20170144161A1

公开(公告)日：2017-05-25

申请号：US15365894

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Kevin D. Ness ,  Billy W. Colston, JR. ,  Fred P. Milanovich ,  Donald A. Masquelier ,  Anthony J. Makarewicz, JR.

IPC: B01L3/00 ,  B01F13/00 ,  G01N21/64 ,  B29C45/00 ,  C12Q1/68 ,  B01L7/00 ,  B01F3/08 ,  B01F15/00

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G06F19/00 ,  Y02A90/26

Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.

公开(公告)号：US20160059204A1

公开(公告)日：2016-03-03

申请号：US14938470

申请日：2015-11-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, JR. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: B01J19/00 ,  G01N1/44 ,  G01N1/30

CPC classification number: B01J19/0046 ,  B01F13/0071 ,  B01F13/0079 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00418 ,  B01J2219/00495 ,  B01J2219/0059 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00873 ,  B01J2219/00894 ,  B01J2219/00903 ,  B01J2219/00957 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/0677 ,  C12Q1/6844 ,  C40B50/08 ,  C40B60/14 ,  G01N1/30 ,  G01N1/44 ,  G01N21/75 ,  G01N33/50 ,  G01N35/08 ,  C12Q2547/107

Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

Abstract translation: 系统，包括方法，装置，组合物和试剂盒，用于通过多个乳液的聚结来混合小体积的流体。