公开(公告)号：US20180179557A1

公开(公告)日：2018-06-28

申请号：US15894581

申请日：2018-02-12

申请人： Gevo, Inc.

发明人： Reid FELDMAN ,  Uvini GUNAWARDENA ,  Jun URANO ,  Peter MEINHOLD ,  Aristos ARISTIDOU ,  Catherine Asleson DUNDON ,  Christopher SMITH

IPC分类号： C12P7/16 ,  C12N9/04 ,  C12N9/90 ,  C12N9/02 ,  C12N15/81 ,  C12N9/88 ,  C12N9/10

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y101/05003 ,  C12Y202/01006 ,  C12Y401/01001 ,  C12Y401/01074 ,  C12Y402/01009 ,  Y02E50/10

摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

公开(公告)号：US20150218596A1

公开(公告)日：2015-08-06

申请号：US14482308

申请日：2014-09-10

申请人： GEVO, INC.

发明人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

IPC分类号： C12P7/16 ,  C12N9/88 ,  C12N9/04 ,  C12N9/10

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y101/05003 ,  C12Y202/01006 ,  C12Y401/01001 ,  C12Y401/01074 ,  C12Y402/01009 ,  Y02E50/10

摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

摘要翻译： 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。

公开(公告)号：US08426165B2

公开(公告)日：2013-04-23

申请号：US12522304

申请日：2008-01-22

申请人： Kwang-myung Cho ,  Kyung-oh Choi ,  Hyun-ae Bae

发明人： Kwang-myung Cho ,  Kyung-oh Choi ,  Hyun-ae Bae

IPC分类号： C12N15/00

CPC分类号： C12P13/14 ,  C12N9/0006 ,  C12N9/1205 ,  C12P13/08 ,  C12Y101/05003 ,  C12Y207/0103

摘要： The present invention relates to a method for producing fermentation product from various carbon sources containing glycerol using Corynebacteria. More particularly, the present invention relates to a method for producing fermentation product from carbon sources containing glycerol or a part of glycerol with high yield and high productivity, by fermenting Corynebacteria introduced with the foreign gene glpDFK facilitating the use of glycerol and accumulating industrially useful amino acids in the culture medium.

摘要翻译： 本发明涉及使用棒杆菌从含有甘油的各种碳源生产发酵产物的方法。 更具体地说，本发明涉及通过发酵引入外源基因glpDFK的棒状杆菌来促进甘油的使用并累积工业上有用的氨基，从含有甘油或一部分甘油的甘油或一部分甘油产生发酵产物的方法 培养基中的酸。

公开(公告)号：US20160333380A1

公开(公告)日：2016-11-17

申请号：US15153492

申请日：2016-05-12

申请人： Samsung Electronics Co., Ltd.

发明人： Soonchun Chung ,  Jinha Kim ,  Kwangmyung Cho

IPC分类号： C12P7/42 ,  C12N15/52 ,  C12N9/88 ,  C12N9/02 ,  C12N15/81 ,  C12N9/04

CPC分类号： C12P7/42 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N15/52 ,  C12N15/81 ,  C12P7/56 ,  C12Y101/01001 ,  C12Y101/01008 ,  C12Y101/01027 ,  C12Y101/01028 ,  C12Y101/01094 ,  C12Y101/02003 ,  C12Y101/02004 ,  C12Y101/05003 ,  C12Y102/01004 ,  C12Y102/0101 ,  C12Y401/01001

摘要： Provided are a genetically engineered yeast cell having increased activity of SUL1, STR3, HXT7, ERR1, GRX8, MXR1, GRE1, MRK1, AAD10 or a combination thereof, compared to a parent cell, and also having acid tolerance, a method of preparing the same, and a method of producing lactate using the same.

摘要翻译： 提供了与亲本细胞相比，具有增加的SUL1，STR3，HXT7，ERR1，GRX8，MXR1，GRE1，MRK1，AAD10或其组合的活性的遗传工程酵母细胞，并且还具有耐酸性，制备 相同，以及使用其制造乳酸盐的方法。

公开(公告)号：US20120171735A1

公开(公告)日：2012-07-05

申请号：US13378479

申请日：2010-06-15

申请人： Anthony John Sinskey ,  Daniel MacEachran ,  Kazuhiko Kurosawa ,  Paolo Boccazzi ,  Jason W. Holder

发明人： Anthony John Sinskey ,  Daniel MacEachran ,  Kazuhiko Kurosawa ,  Paolo Boccazzi ,  Jason W. Holder

IPC分类号： C12P7/64 ,  C12N9/02 ,  C07K14/195 ,  C12N1/21

CPC分类号： C12N15/8247 ,  A61K39/05 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1205 ,  C12P7/6463 ,  C12Y101/05003 ,  C12Y207/0103

摘要： The invention relates to production of triacylglycerols in cells.

摘要翻译： 本发明涉及细胞内三酰基甘油的生产。

公开(公告)号：US20120028323A1

公开(公告)日：2012-02-02

申请号：US13267771

申请日：2011-10-06

申请人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

发明人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

IPC分类号： C12P7/16 ,  C12N1/19

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y101/05003 ,  C12Y202/01006 ,  C12Y401/01001 ,  C12Y401/01074 ,  C12Y402/01009 ,  Y02E50/10

摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

摘要翻译： 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。

公开(公告)号：US20110020889A1

公开(公告)日：2011-01-27

申请号：US12820505

申请日：2010-06-22

申请人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

发明人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

IPC分类号： C12P7/16 ,  C12N1/19

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y101/05003 ,  C12Y202/01006 ,  C12Y401/01001 ,  C12Y401/01074 ,  C12Y402/01009 ,  Y02E50/10

摘要： The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

摘要翻译： 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可以包含导致丙酮酸脱羧酶和/或甘油-3-磷酸脱氢酶活性降低的修饰。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。

公开(公告)号：US10041094B2

公开(公告)日：2018-08-07

申请号：US14233830

申请日：2011-07-22

申请人： R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Hu

发明人： R. Rogers Yocum ,  Theron Hermann ,  Xiaohui Hu

IPC分类号： C12P7/46 ,  C12N1/20 ,  C12N15/52 ,  C07K14/245 ,  C12N9/04 ,  C12N9/12 ,  C12P7/56

CPC分类号： C12P7/46 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/52 ,  C12P7/56 ,  C12Y101/01008 ,  C12Y101/05003

摘要： The present invention is in the field of producing organic acids and other useful chemicals via biological fermentation using glycerol as a source of carbon. Novel microorganisms and fermentation processes are described that are capable of converting glycerol to useful organic acids in high yield and high purity.

公开(公告)号：US09926577B2

公开(公告)日：2018-03-27

申请号：US14482308

申请日：2014-09-10

申请人： GEVO, INC.

发明人： Reid M. Renny Feldman ,  Uvini Gunawardena ,  Jun Urano ,  Peter Meinhold ,  Aristos Aristidou ,  Catherine Asleson Dundon ,  Christopher Smith

IPC分类号： C12N1/19 ,  C12N15/81 ,  C12P7/16 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N9/60

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01008 ,  C12Y101/01086 ,  C12Y101/05003 ,  C12Y202/01006 ,  C12Y401/01001 ,  C12Y401/01074 ,  C12Y402/01009 ,  Y02E50/10

摘要： The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and/or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

公开(公告)号：US20160376613A1

公开(公告)日：2016-12-29

申请号：US15228770

申请日：2016-08-04

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： RITU BHALLA ,  GOPAL K. CHOTANI ,  MICHAEL DAUNER ,  MARK J. NELSON ,  DANIEL P. O'KEEFE ,  CAROLINE M. PERES ,  JAHNAVI CHANDRA PRASAD ,  JEAN-FRANCOIS TOMB

IPC分类号： C12P7/16 ,  C12N9/02

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/52 ,  C12Y101/01008 ,  C12Y101/01094 ,  C12Y101/05003 ,  C12Y102/01012 ,  Y02E50/10 ,  Y02P20/52

摘要： Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

摘要翻译： 本文提供了对NADH和GPD酶具有增加的KM的甘油-3-磷酸脱氢酶（GPD）酶，对NADH和NADPH具有基本上相同的亲和力和/或被甘油-3-磷酸抑制的反馈。 本文还提供了包含编码GPD的异源基因和编码GPD的内源基因中的缺失或破坏的重组微生物。 还提供了包含编码GPD的异源基因和丁醇生物合成途径的重组微生物。 还提供了生产丁醇的方法，包括提供本文所述的重组微生物，并且在产生丁醇的条件下使重组微生物与至少一种可发酵碳底物接触。