公开(公告)号：US20160376613A1

公开(公告)日：2016-12-29

申请号：US15228770

申请日：2016-08-04

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： RITU BHALLA ,  GOPAL K. CHOTANI ,  MICHAEL DAUNER ,  MARK J. NELSON ,  DANIEL P. O'KEEFE ,  CAROLINE M. PERES ,  JAHNAVI CHANDRA PRASAD ,  JEAN-FRANCOIS TOMB

IPC分类号： C12P7/16 ,  C12N9/02

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N15/52 ,  C12Y101/01008 ,  C12Y101/01094 ,  C12Y101/05003 ,  C12Y102/01012 ,  Y02E50/10 ,  Y02P20/52

摘要： Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

摘要翻译： 本文提供了对NADH和GPD酶具有增加的KM的甘油-3-磷酸脱氢酶（GPD）酶，对NADH和NADPH具有基本上相同的亲和力和/或被甘油-3-磷酸抑制的反馈。 本文还提供了包含编码GPD的异源基因和编码GPD的内源基因中的缺失或破坏的重组微生物。 还提供了包含编码GPD的异源基因和丁醇生物合成途径的重组微生物。 还提供了生产丁醇的方法，包括提供本文所述的重组微生物，并且在产生丁醇的条件下使重组微生物与至少一种可发酵碳底物接触。