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公开(公告)号：US20180003672A1
公开(公告)日：2018-01-04
申请号：US15704695
申请日：2017-09-14
Applicant: Life Technologies Corporation
Inventor： James Ball , Brian Reed
CPC classification number: G01N27/4145 , B08B3/08 , B08B9/08 , C11D3/162 , C11D3/3418 , C11D3/43 , C11D7/34 , C11D7/5013 , C11D7/5022 , C11D7/5027
Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.
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公开(公告)号：US09534215B2
公开(公告)日：2017-01-03
申请号：US14736734
申请日：2015-06-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed
CPC classification number: C12N15/1013 , C07H1/06 , C12Q1/6806 , C12Q1/6844 , C12Q2563/143 , C12Q2563/157 , C12Q2565/519
Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.
Abstract translation: 分离珠粒基材的方法包括将乳液施加到破乳溶液中。 乳液的分散相包括未结合的多核苷酸，第一组珠粒基质和第二组珠粒基底。 未结合的多核苷酸包括与偶联寡核苷酸互补的区段。 第一组珠底物包括扩增为包含与未结合多核苷酸的一部分互补的区段的偶联寡核苷酸。 第二组珠底物包括偶联寡核苷酸。 破乳溶液包括具有与偶联寡核苷酸相似的序列或与偶联寡核苷酸互补的序列的干扰探针。 该方法还包括将第一组胎圈衬底的珠粒与分离衬底结合，并将第二组胎圈衬底的未结合的珠粒与与分离衬底结合的第一组胎圈衬底的珠粒分离。
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公开(公告)号：US20220193576A1
公开(公告)日：2022-06-23
申请号：US17566511
申请日：2021-12-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed , James A. Ball
IPC: B01D17/04 , C07H1/06 , C07H21/00 , C12Q1/6806
Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
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公开(公告)号：US10576396B2
公开(公告)日：2020-03-03
申请号：US15375271
申请日：2016-12-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed , James A. Ball
IPC: C12Q1/6806 , B01D17/04 , C07H1/06 , C07H21/00
Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
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公开(公告)号：US09458485B2
公开(公告)日：2016-10-04
申请号：US14799259
申请日：2015-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech , John Andrew Sheridan
IPC: C12P19/34 , C12M1/26 , B01F15/00 , B04B11/02 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , B01F3/08 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
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Patent Agency Ranking
公开(公告)号：US11833450B2
公开(公告)日：2023-12-05
申请号：US17566511
申请日：2021-12-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed , James A. Ball
IPC: B01D17/04 , C07H1/06 , C07H21/00 , C12Q1/6806
CPC classification number: B01D17/047 , B01D17/04 , B01D17/041 , C07H1/06 , C07H21/00 , C12Q1/6806 , C12Q1/6806 , C12Q2527/125 , C12Q2527/137 , C12Q2563/159
Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
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公开(公告)号：US10184146B2
公开(公告)日：2019-01-22
申请号：US15387525
申请日：2016-12-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed
IPC: C07H1/06 , C12Q1/6806 , C12N15/10 , C12Q1/6844
Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.
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公开(公告)号：US09121047B2
公开(公告)日：2015-09-01
申请号：US13775855
申请日：2013-02-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Thwar , Todd Roswech , Andrew Sheridan
IPC: C12M1/00 , C12M3/00 , C12M1/34 , C12M1/36 , C12M1/38 , C12P19/34 , C12M1/26 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。
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公开(公告)号：US20140201172A1
公开(公告)日：2014-07-17
申请号：US14154240
申请日：2014-01-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed
IPC: G06F17/30
CPC classification number: G16B30/00 , C12Q1/6874
Abstract: Systems and method for identifying duplicate reads can receive first and second reads, determine if the first and second reads have a same start and end position, determine a binary flow difference, and identify the second read as a duplicate of the first read when the binary flow difference exceeds a threshold.
Abstract translation: 用于识别重复读取的系统和方法可以接收第一和第二读取，确定第一和第二读取是否具有相同的开始和结束位置，确定二进制流差异，并且当二进制文件的二进制 流量差超过阈值。
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公开(公告)号：US11773426B2
公开(公告)日：2023-10-03
申请号：US16514814
申请日：2019-07-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： David Joun , Chieh-Yuan Li , Brian Reed , Craig Obergfell , Devin Dressman , Abraham Rosenbaum , Scott Benson , Andi Broka , Srinka Ghosh
IPC: C12Q1/68 , C12Q1/6806 , C12Q1/6844
CPC classification number: C12Q1/6806 , C12Q1/6844 , C12Q1/6846 , C12Q1/6844 , C12Q2537/143 , C12Q2563/149 , C12Q2565/537 , C12Q1/6844 , C12Q2525/191 , C12Q2537/143 , C12Q2563/149 , C12Q2565/537
Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.
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