公开(公告)号：US10240212B2

公开(公告)日：2019-03-26

申请号：US15388750

申请日：2016-12-22

申请人： GeneWeave Biosciences, Inc.

发明人： Diego Ariel Rey ,  Shaunak Roy ,  Leonardo Maestri Teixeira ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor H. Yee ,  Werner Frei

IPC分类号： C12Q1/06 ,  C12Q1/10 ,  C12Q1/66 ,  C12Q1/68 ,  C12Q1/6897 ,  C12Q1/04 ,  B01L3/00 ,  G01N21/01 ,  G01N21/76 ,  G01N21/03 ,  G01N21/13 ,  B01L7/00 ,  C12Q1/02 ,  G01J1/00 ,  C12Q1/14 ,  G01N35/00 ,  G01N35/04

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

公开(公告)号：US09481903B2

公开(公告)日：2016-11-01

申请号：US13802461

申请日：2013-03-13

申请人： GeneWeave Biosciences, Inc.

发明人： Diego Ariel Rey ,  Shaunak Roy ,  Leonardo Maestri Teixeira ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor Yee

IPC分类号： C12Q1/70 ,  C12Q1/66 ,  C12Q1/02 ,  C12Q1/68 ,  G01N21/00 ,  C12Q1/04 ,  B01L3/00 ,  G01N21/01 ,  G01N21/76 ,  G01N21/03 ,  G01N21/13 ,  B01L7/00 ,  G01J1/00 ,  G01N35/00 ,  G01N35/04

CPC分类号： C12Q1/6897 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/52 ,  B01L3/523 ,  B01L3/54 ,  B01L3/545 ,  B01L7/00 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2400/0478 ,  B01L2400/06 ,  B01L2400/0683 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/66 ,  G01J1/00 ,  G01N21/01 ,  G01N21/03 ,  G01N21/13 ,  G01N21/76 ,  G01N21/763 ,  G01N2035/00346 ,  G01N2035/0405 ,  G01N2333/195

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

公开(公告)号：US20140272928A1

公开(公告)日：2014-09-18

申请号：US13802461

申请日：2013-03-13

申请人： GeneWeave Biosciences, Inc.

发明人： Diego Ariel REY ,  Shaunak Roy ,  Leonardo Maestri Teixeira ,  Ryan C. Griswold ,  Kenneth G. Olson ,  Bruce J. Richardson ,  Victor Yee

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6897 ,  B01L3/502 ,  B01L3/50825 ,  B01L3/52 ,  B01L3/523 ,  B01L3/54 ,  B01L3/545 ,  B01L7/00 ,  B01L2200/028 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/047 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2400/0478 ,  B01L2400/06 ,  B01L2400/0683 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/66 ,  G01J1/00 ,  G01N21/01 ,  G01N21/03 ,  G01N21/13 ,  G01N21/76 ,  G01N21/763 ,  G01N2035/00346 ,  G01N2035/0405 ,  G01N2333/195

摘要： Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

摘要翻译： 本文描述了使用工程化转导颗粒检测和/或鉴定靶细胞（例如细菌）的系统和方法。 在一些实施方案中，一种方法包括将一定量的转导颗粒混合在样品内。 转导颗粒与靶细胞相关联。 转导颗粒是非复制型的，并被工程化以包括配制成使靶细胞产生一系列报道分子的核酸分子。 当靶细胞存在于样品中时，保持样品和转导颗粒以表达一系列报告分子。 接收与一定量的报道分子相关的信号。 在一些实施例中，信号的幅度与高于预定量的转导颗粒的数量无关。