公开(公告)号：US10597737B2

公开(公告)日：2020-03-24

申请号：US16109288

申请日：2018-08-22

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Methods and kits for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. At least one of the primers is configured to hybridize to a region of the polynucleotide analyte encoding the genetic variation. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US09618477B2

公开(公告)日：2017-04-11

申请号：US13967108

申请日：2013-08-14

Applicant: California Institute of Technology

Inventor： Sameer Walavalkar ,  Axel Scherer ,  Thomas A. Tombrello ,  Aditya Rajagopal ,  Andrew P. Homyk ,  Erika Garcia

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487 ,  B82Y30/00

CPC classification number: G01N27/447 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Methods and devices for sequencing nucleic acids are disclosed herein. Devices are also provided herein for measuring DNA with nano-pores sized to allow DNA to pass through the nano-pore. The capacitance can be measured for the DNA molecule passing through the nano-pore. The capacitance measurements can be correlated to determine the sequence of base pairs passing through the nano-pore to sequence the DNA.

公开(公告)号：US20230151440A1

公开(公告)日：2023-05-18

申请号：US17810696

申请日：2022-07-05

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/703 ,  C12Q1/68 ,  C12Q1/6851 ,  Y10T436/143333

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US11414716B2

公开(公告)日：2022-08-16

申请号：US16827590

申请日：2020-03-23

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US09390936B2

公开(公告)日：2016-07-12

申请号：US14527039

申请日：2014-10-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  Stephanie Tombrello

Inventor： Michael D. Henry ,  Andrew P. Homyk ,  Axel Scherer ,  Thomas A. Tombrello ,  Sameer Walavalkar

IPC: G01N1/18 ,  H01L21/308 ,  B81C1/00 ,  H01L21/3065 ,  G01N1/40

CPC classification number: B81C1/00111 ,  B01J19/0046 ,  B01J2219/00504 ,  B01J2219/00664 ,  B81B1/006 ,  B81B2203/0361 ,  B81B2207/056 ,  B81C2201/0112 ,  B81C2201/0132 ,  B81C2201/0133 ,  B81C2201/0159 ,  G01N1/405 ,  H01L21/3065 ,  H01L21/30655 ,  H01L21/3081 ,  H01L21/3086 ,  Y10T436/25375

Abstract: Methods for fabricating of high aspect ratio probes and deforming micropillars and nanopillars are described. Use of polymers in deforming nanopillars and micropillars is also described.

公开(公告)号：US08841712B2

公开(公告)日：2014-09-23

申请号：US13852480

申请日：2013-03-28

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Axel Scherer ,  Michael D. Henry ,  Sameer Walavalkar ,  Thomas A. Tombrello ,  Andrew P. Homyk

IPC: H01L29/76 ,  G01N27/327 ,  H01L29/66 ,  H01L29/78

CPC classification number: C12Q1/02 ,  A61B5/04001 ,  G01N27/3275 ,  G01N27/4146 ,  G01R1/06711 ,  G01R1/07342 ,  H01L29/413 ,  H01L29/42376 ,  H01L29/4966 ,  H01L29/66477 ,  H01L29/78 ,  H01L29/7831

Abstract: A field effect nano-pillar transistor has a pillar shaped gate element incorporating a biomimitec portion that provides various advantages over prior art devices. The small size of the nano-pillar transistor allows for advantageous insertion into cellular membranes, and the biomimitec character of the gate element operates as an advantageous interface for sensing small amplitude voltages such as transmembrane cell potentials. The nano-pillar transistor can be used in various embodiments to stimulate cells, to measure cell response, or to perform a combination of both actions.

Abstract translation: 场效应纳米柱晶体管具有结合有生物医学部分的柱形门元件，其提供了优于现有技术器件的各种优点。 纳米柱晶体管的小尺寸允许有利地插入到细胞膜中，并且栅极元件的生物学特性作为用于感测小幅度电压（例如跨膜电池电位）的有利接口。 纳米柱晶体管可用于各种实施例中以刺激细胞，测量细胞反应或执行两种动作的组合。

公开(公告)号：US11879162B2

公开(公告)日：2024-01-23

申请号：US17810696

申请日：2022-07-05

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q1/68 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2565/101

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US20210002732A1

公开(公告)日：2021-01-07

申请号：US16827590

申请日：2020-03-23

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US09846125B2

公开(公告)日：2017-12-19

申请号：US14621295

申请日：2015-02-12

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Sameer Walavalkar ,  Axel Scherer ,  Thomas A. Tombrello

IPC: G01N21/65 ,  G01N33/00 ,  G01N33/18 ,  G01N33/483 ,  A61B5/00

CPC classification number: G01N21/658 ,  A61B5/00 ,  A61B5/0031 ,  A61B5/14503 ,  A61B5/1455 ,  A61B5/14552 ,  A61B5/1459 ,  A61B5/686 ,  G01N33/0004 ,  G01N33/18 ,  G01N33/483

Abstract: Methods and systems for nanopillar sensors are described. Nanopillars can be defined on a substrate, and metal deposited on the nanopillars. A thermal treatment can reflow the metal on the nanopillars forming metallic bulbs on the top end of the nanopillars. These structures can have enhanced optical detection when functionalized with biological agents, or can detect gases, particles and liquids through interaction with the metal layer on the nanopillars.

公开(公告)号：US09243277B2

公开(公告)日：2016-01-26

申请号：US14753741

申请日：2015-06-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Aditya Rajagopal ,  Axel Scherer ,  Michael D. Henry ,  Sameer Walavalkar ,  Thomas A. Tombrello ,  Andrew P. Homyk

IPC: G01R1/067 ,  G01R31/00 ,  C12Q1/02 ,  H01L29/78 ,  H01L29/66 ,  G01N27/327 ,  G01N27/414 ,  A61B5/04 ,  H01L29/41 ,  H01L29/423 ,  H01L29/49 ,  G01R1/073

CPC classification number: C12Q1/02 ,  A61B5/04001 ,  G01N27/3275 ,  G01N27/4146 ,  G01R1/06711 ,  G01R1/07342 ,  H01L29/413 ,  H01L29/42376 ,  H01L29/4966 ,  H01L29/66477 ,  H01L29/78 ,  H01L29/7831

Abstract: The basic structure and functionality of a probe as disclosed herein allows for flexibly incorporating into the probe, various sensing elements for various sensing applications. Two example applications among these various sensing applications include bio-sensing and chemical-sensing applications. For bio-sensing applications the probe, which is fabricated upon a silicon substrate, includes a bio-sensing element such as a nano-pillar transistor, and for chemical-sensing applications the probe includes a sensing element that has a functionalized contact area whereby the sensing element generates a voltage when exposed to one or more chemicals of interest.