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公开(公告)号：US11007520B2
公开(公告)日：2021-05-18
申请号：US16196649
申请日：2018-11-20
发明人： Randall D. Lowe, Jr. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC分类号： B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
摘要： In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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2.发明申请公开(公告)号：US20210011015A1
公开(公告)日：2021-01-14
申请号：US16908265
申请日：2020-06-22
发明人： Kristin G. Beaumont , Peter J. Beemiller , Volker L.S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号： G01N33/543 , B01L3/00 , G01N33/545 , G01N33/58
摘要： In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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3.发明授权公开(公告)号：US11454629B2
公开(公告)日：2022-09-27
申请号：US16908265
申请日：2020-06-22
发明人： Kristin G. Beaumont , Peter J. Beemiller , Volker L. S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号： B01L3/00 , G01N33/543 , G01N33/545 , G01N33/58 , G01N33/50
摘要： In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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公开(公告)号：US11365381B2
公开(公告)日：2022-06-21
申请号：US16920365
申请日：2020-07-02
发明人： Randall D. Lowe, Jr. , Kristin G. Beaumont , Aathavan Karunakaran , Natalie C. Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
摘要： Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
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5.发明申请公开(公告)号：US20170184583A1
公开(公告)日：2017-06-29
申请号：US15372094
申请日：2016-12-07
发明人： Kristin G. Beaumont , Peter J. Beemiller , Volker L.S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号： G01N33/543 , G01N33/58 , G01N33/545
摘要： In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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公开(公告)号：US20230182136A1
公开(公告)日：2023-06-15
申请号：US18164124
申请日：2023-02-03
发明人： Kristin G. Beaumont , Non-Linda Ding , Volker L.S. Kurz , Troy A. Lionberger , Randall D. Lowe, JR. , Daniele Malleo , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
IPC分类号： B01L3/00 , B01J19/00 , G01N27/447
CPC分类号： B01L3/502753 , B01L3/502761 , B01L3/502738 , B01J19/0093 , B01L3/5023 , B01L3/502707 , G01N27/44791 , B01L2400/0424 , B01L2300/0816 , B01L2300/0864 , B01L2400/0677 , B01L2200/0668 , B01L2300/16 , B01L2400/08
摘要： In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
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公开(公告)号：US20190275516A1
公开(公告)日：2019-09-12
申请号：US16196649
申请日：2018-11-20
发明人： Randall D. Lowe, JR. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
摘要： In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号：US20180135011A1
公开(公告)日：2018-05-17
申请号：US15802100
申请日：2017-11-02
发明人： Yelena Bronevetsky , Xiaohua Wang , Peter J. Beemiller , Kristin G. Beaumont , Randall D. Lowe, JR. , Alexander J. Mastroianni , Kevin T. Chapman
IPC分类号： C12N5/0783 , A61P35/00 , C12M3/06 , A61K39/00 , C12M1/00
CPC分类号： C12N5/0636 , A61K35/17 , A61K39/0011 , A61K2035/124 , A61K2039/5158 , A61P35/00 , C12M23/16 , C12M29/10 , C12N2501/2302 , C12N2501/51 , C12N2501/515 , C12N2502/1121 , C12N2533/50
摘要： Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.
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公开(公告)号：US11801508B2
公开(公告)日：2023-10-31
申请号：US17162467
申请日：2021-01-29
发明人： Randall D. Lowe, Jr. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC分类号： B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
CPC分类号： B01L3/502707 , B01L3/502715 , B01L3/502761 , B81C1/00206 , C12M23/16 , G01N33/54393 , B01L2300/0636 , B01L2300/088 , B01L2300/0816 , B01L2300/0864 , B81B2201/058
摘要： In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号：US11666913B2
公开(公告)日：2023-06-06
申请号：US15359115
申请日：2016-11-22
发明人： Kristin G. Beaumont , Nan-Linda Ding , Volker L. S. Kurz , Troy A. Lionberger , Randall D. Lowe, Jr. , Daniele Malleo , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
IPC分类号： B01L3/00 , B01J19/00 , G01N27/447
CPC分类号： B01L3/502753 , B01J19/0093 , B01L3/5023 , B01L3/502707 , B01L3/502738 , B01L3/502761 , G01N27/44791 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/16 , B01L2400/0424 , B01L2400/0677 , B01L2400/08
摘要： In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
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