公开(公告)号：US20190283026A1

公开(公告)日：2019-09-19

申请号：US16253869

申请日：2019-01-22

申请人： Berkeley Lights, Inc.

发明人： Kevin D. Loutherback ,  Yelena Bronevetsky ,  Peter J. Beemiller ,  Xiaohua Wang ,  Kevin T. Chapman

IPC分类号： B01L3/00 ,  G01N1/34 ,  A61K35/17

摘要： Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

公开(公告)号：US10705082B2

公开(公告)日：2020-07-07

申请号：US15372094

申请日：2016-12-07

申请人： Berkeley Lights, Inc.

发明人： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L. S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC分类号： B01L3/00 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58

摘要： In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20210011015A1

公开(公告)日：2021-01-14

申请号：US16908265

申请日：2020-06-22

申请人： Berkeley Lights, Inc.

发明人： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L.S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC分类号： G01N33/543 ,  B01L3/00 ,  G01N33/545 ,  G01N33/58

摘要： In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20230313107A1

公开(公告)日：2023-10-05

申请号：US18147356

申请日：2022-12-28

申请人： BERKELEY LIGHTS, INC.

发明人： Andrew W. McFarland ,  Peter J. Beemiller ,  Guido K. Stadler ,  Alexander J. Mastroianni ,  Joshua J. Cardiel Rivera ,  Darcy K. Kelly-Greene ,  Jonathan Cloud Dragon Hubbard ,  Natalie C. Marks ,  Long Van Le ,  Ke-Chih Lin

IPC分类号： C12M1/00 ,  C12M3/06 ,  C12M3/00 ,  C12M1/34 ,  C12N5/0783

CPC分类号： C12M29/10 ,  C12M23/16 ,  C12M23/40 ,  C12M23/42 ,  C12M41/12 ,  C12M41/36 ,  C12N5/0636 ,  B01L2300/0883

摘要： Cartridge for manufacturing a population of cells suitable for formulation as a cellular therapeutic are disclosed herein, along with systems for operating the cartridges and performing methods to generate the population of cells suitable for formulation as a cellular therapeutic. The population of cells suitable for formulation as a cellular therapeutic can be T-cells, including CAR T-cells. The systems and methods can be largely automated.

公开(公告)号：US20220325240A1

公开(公告)日：2022-10-13

申请号：US17574459

申请日：2022-01-12

申请人： Berkeley Lights, Inc.

发明人： Andrew W. McFarland ,  Peter J. Beemiller ,  Guido K. Stadler ,  Alexander J. Mastroianni ,  Joshua J. Cardiel Rivera ,  Darcy K. Kelly-Greene ,  Jonathan Cloud Dragon Hubbard ,  Natalie C. Marks ,  Long Van Le ,  Ke-Chih Lin

IPC分类号： C12N5/0783 ,  C12N15/62 ,  C12N15/86 ,  C12M3/00 ,  C12M1/42 ,  C12M1/00

摘要： Cartridges for manufacturing a population of cells suitable for formulation as a cellular therapeutic are disclosed herein, along with systems and instruments for operating the cartridges and performing methods to generate the population of cells suitable for formulation as a cellular therapeutic. The population of cells suitable for formulation as a cellular therapeutic can be immunological cells, such as T lymphocytes, including endogenous T cells (ETCs), tumor infiltrating lymphocytes (TILs), CAR T-cells, TCR engineered T-cells, or otherwise engineered T-cells. The systems and methods can be largely automated.

公开(公告)号：US11454629B2

公开(公告)日：2022-09-27

申请号：US16908265

申请日：2020-06-22

申请人： Berkeley Lights, Inc.

发明人： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L. S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC分类号： B01L3/00 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  G01N33/50

摘要： In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20170184583A1

公开(公告)日：2017-06-29

申请号：US15372094

申请日：2016-12-07

申请人： Berkeley Lights, Inc.

发明人： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L.S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/545

摘要： In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US11666912B2

公开(公告)日：2023-06-06

申请号：US16253869

申请日：2019-01-22

申请人： Berkeley Lights, Inc.

发明人： Kevin D. Loutherback ,  Yelena Bronevetsky ,  Peter J. Beemiller ,  Xiaohua Wang ,  Kevin T. Chapman

IPC分类号： B01L3/00 ,  A61K35/17 ,  G01N1/34 ,  B03C5/02 ,  A61K35/28 ,  B03C5/00 ,  G01N1/40

CPC分类号： B01L3/502746 ,  A61K35/17 ,  A61K35/28 ,  B01L3/502761 ,  B01L3/502792 ,  B03C5/005 ,  B03C5/026 ,  G01N1/34 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C2201/26 ,  G01N2001/4088

摘要： Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.

公开(公告)号：US20200299351A1

公开(公告)日：2020-09-24

申请号：US16743849

申请日：2020-01-15

申请人： Berkeley Lights, Inc.

发明人： Peter J. Beemiller ,  Alexander J. Mastroianni ,  Shao Ning Pei ,  Randall D. Lowe, JR. ,  Annamaria Mocciaro ,  Kevin D. Loutherback ,  Yelena Bronevetsky ,  Guido K. Stadler ,  Andrew W. McFarland ,  Kevin T. Chapman ,  Duane Smith ,  Natalie C. Marks ,  Amanda L. Goodsell

IPC分类号： C07K14/725 ,  C07K16/28 ,  G01N33/50 ,  C07K14/74

摘要： In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

公开(公告)号：US20180135011A1

公开(公告)日：2018-05-17

申请号：US15802100

申请日：2017-11-02

申请人： Berkeley Lights, Inc.

发明人： Yelena Bronevetsky ,  Xiaohua Wang ,  Peter J. Beemiller ,  Kristin G. Beaumont ,  Randall D. Lowe, JR. ,  Alexander J. Mastroianni ,  Kevin T. Chapman

IPC分类号： C12N5/0783 ,  A61P35/00 ,  C12M3/06 ,  A61K39/00 ,  C12M1/00

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61K39/0011 ,  A61K2035/124 ,  A61K2039/5158 ,  A61P35/00 ,  C12M23/16 ,  C12M29/10 ,  C12N2501/2302 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2502/1121 ,  C12N2533/50

摘要： Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.