公开(公告)号：US20240133881A1

公开(公告)日：2024-04-25

申请号：US18491674

申请日：2023-10-19

申请人： Quidel Corporation

发明人： Valerio Pini ,  Adonis Stassinopoulos ,  Matthias Mösl ,  Jesús Óscar Ahumada Heredero ,  Chloé Alexia Rodriguez ,  Andreas Thon ,  Houman Yaghoubi ,  Scott Rongey

IPC分类号： G01N33/558 ,  B82Y30/00

CPC分类号： G01N33/558 ,  B82Y30/00

摘要： Disclosed are various embodiments of a device comprising a synthetic polymeric substrate having a high quality finish upper surface, the upper surface having at least a bilayer coating comprising a first, reflective layer and a second, transparent layer. Also disclosed are kits containing embodiments of the disclosed device and detectable particles. Also disclosed are various embodiments of a method of using the disclosed device and various embodiments of a method of using the disclosed kit.

公开(公告)号：US11726078B2

公开(公告)日：2023-08-15

申请号：US17091501

申请日：2020-11-06

申请人： General Electric Company ,  Tokitae LLC

发明人： Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Cathryn Ellen Olsen ,  John Richard Nelson ,  David Roger Moore ,  Paul Michael Smigelski, Jr. ,  John Thomas Connelly ,  Benjamin David Grant ,  Bernhard Hans Weigl

IPC分类号： G01N33/493 ,  C07K1/22 ,  B01D15/08 ,  G01N33/50 ,  G01N33/569 ,  B01D63/02 ,  A61B10/00 ,  C12M1/26 ,  G01N33/558 ,  G01N15/14

CPC分类号： G01N33/493 ,  A61B10/007 ,  B01D15/08 ,  B01D63/02 ,  C07K1/22 ,  C12M33/14 ,  G01N33/5038 ,  G01N33/558 ,  G01N33/569 ,  G01N33/5695 ,  A61M2202/005 ,  A61M2202/0028 ,  A61M2202/0071 ,  A61M2202/0496 ,  G01N2015/1493

摘要： A device for rapid detection of a tuberculosis lipoarabinomannan (TB-LAM) is provided. The device includes a pre-concentrator unit for concentrating the TB-LAM comprising: an ion-exchange medium comprising one or more ligands configured to capture the TB-LAM from the source biological sample, wherein the captured-TB-LAM is eluted from the ion-exchange medium as an eluate comprising a concentrated form of TB-LAM; a cassette; a lateral flow assay unit disposed in the cassette; and an integration unit attached to the pre-concentrator unit and the cassette. The integration unit is configured to operatively couple and de-couple the pre-concentrator unit and the cassette. The pre-concentrator unit and the lateral flow assay unit disposed in the cassette are in a fluidic communication in a coupled form. The device for rapid detection of TB-LAM further comprises a dilutor unit.

公开(公告)号：US11635432B2

公开(公告)日：2023-04-25

申请号：US16751114

申请日：2020-01-23

申请人： The Regents of the University of California

发明人： Daniel Takashi Kamei ,  Yin To Chiu ,  Benjamin Ming Wu ,  Garrett L. Mosley

IPC分类号： G01N33/558 ,  G01N33/543

摘要： Disclosed herein are devices and methods that use aqueous two phase systems and lateral flow assays to detect target analytes in a sample. These devices and methods may be used to diagnose a disease or condition in a biological sample, such as blood or serum. In addition, these devices and methods may be used to detect allergens in a food samples or contaminants, such as environmental toxins, in water samples. Device and kit components may be conveniently assembled in a portable container and are amenable to actuation in most settings. The devices are simple to use, requiring a non-trained operator to simply add the sample to the device. Conveniently, the time it takes to detect the target analyte is very short. Thus, the devices and methods disclosed herein provide novel and useful means for point-of-care.

公开(公告)号：US20230081132A1

公开(公告)日：2023-03-16

申请号：US17884792

申请日：2022-08-10

申请人： Jiangnan University

发明人： Yongli YE ,  Xiulan SUN ,  Zhixuan SHI ,  Jiadi SUN ,  Jian JI ,  Yinzhi ZHANG

IPC分类号： G01N33/543 ,  G01N33/558 ,  G01N33/533

摘要： The present invention discloses a time-resolved immunoquantitation test strip for detecting tetrodotoxin (TTX) in shellfish food, which belongs to the technical field of rapid detection of time-resolved immunoassay. In the present invention, fluorescent microspheres are adopted to replace the traditional colloidal gold; the fluorescent microsphere is used for labeling a TTX antibody complex; by utilizing a competitive immunization method, the fluorescent microsphere, serving as a fluorescent probe, is used for immunochromatography; and by reading the fluorescence value of a detection line on a fluoroimmunoassay instrument, the TTX in shellfish samples can be analyzed quantitatively and rapidly. The time-resolved immunoquantitation test strip of the present invention can be used for detecting the content of the TTX in various types of shellfish food quantitatively and rapidly and is strong in specificity and high in sensitivity, wherein when the concentration of the TTX is 0.5-40 ng/mL, the logarithmic value of the concentration has a linear relationship with T/To, a linear equation is: Y=0.57365-0.2668LgX, R2=0.9940, and the limit of detection can reach 0.047 ng/mL.

公开(公告)号：US20230067165A1

公开(公告)日：2023-03-02

申请号：US18053052

申请日：2022-11-07

申请人： Xtrava, Inc.

发明人： Sameh Sarhan ,  Lawrence H. Zuckerman

IPC分类号： G01N33/558 ,  G01D5/14 ,  G01R29/14 ,  G01R33/09 ,  G01R33/12

摘要： A method for reading a lateral flow assay test strip comprises providing a microwave antenna in proximity of a stain line region of the test strip. The method includes causing a chemical compound to travel to the stain line region, an amount of the chemical compound varying in accordance with a quantity of an analyte. The method comprises connecting an instrumentation to the microwave antenna. The method includes measuring a feed impedance of the microwave antenna using the instrumentation, the feed impedance of the microwave antenna varying with the amount of the chemical compound. The method comprises determining the quantity of the analyte based on the feed impedance of the microwave antenna.

公开(公告)号：US20230032349A1

公开(公告)日：2023-02-02

申请号：US17888396

申请日：2022-08-15

申请人： Keith Rubin ,  Ken Solovay ,  James Layer ,  Alfred A. Desimone ,  Bin Yan

发明人： Keith Rubin ,  Ken Solovay ,  James Layer ,  Alfred A. Desimone ,  Bin Yan

IPC分类号： A61B10/00 ,  G01N33/558 ,  A61B5/1468 ,  A61B5/145 ,  A61B5/00 ,  A61M3/02 ,  G01N33/487 ,  G01N33/543 ,  G01N33/569

摘要： A nasal irrigation diagnostic assembly comprising an irrigation device including a fluid collection portion structured to retain a biological sample, in the form of waste solution from the nasal cavity resulting from irrigation. A detection member disposed on said irrigation device is exposed to the biological sample and is structured to determine the existence of at least one analyte within the biological sample of the waste solution. The detection member comprises a plurality of detection zones individually structured to analyze the biological sample upon engagement therewith, wherein said plurality of zones include at least a reaction zone and a detection zone, which respectively include reagents cooperatively and collectively formulated to detect the existence of the at least one analyte within biological sample of the waste solution. A control zone may also be included to indicate the intended operability of at least the detection member.

公开(公告)号：US11566244B2

公开(公告)日：2023-01-31

申请号：US16703190

申请日：2019-12-04

申请人： ABBOTT DIAGNOSTICS SCARBOROUGH, INC.

发明人： Olaf Piepenburg ,  Colin H. Williams ,  Niall A. Armes

IPC分类号： C12Q1/68 ,  C12N15/113 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6804 ,  G01N21/78 ,  G01N33/558 ,  G01N21/77

摘要： This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.

公开(公告)号：US11565259B2

公开(公告)日：2023-01-31

申请号：US16683798

申请日：2019-11-14

申请人： Berkeley Lights, Inc.

发明人： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC分类号： B01L3/00 ,  G01N33/558

摘要： A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US11561229B2

公开(公告)日：2023-01-24

申请号：US15988939

申请日：2018-05-24

申请人： Hoffmann-La Roche Inc.

发明人： Kay-Gunnar Stubenrauch

IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/558 ,  G01N33/543

摘要： Herein is reported a method for the determination of the amount of a bivalent antibody in a serum or plasma sample obtained from a non-human experimental animal, whereby the antibody comprises one or more mutations in the Fc-region compared to the corresponding wild-type Fc-region that has a sequence of SEQ ID NO: 01, 02, or 03, wherein the method comprises the following steps a) immobilizing a non-antibody polypeptide to which more than one copy of the antigen of the antibody is covalently conjugated on a solid surface, b) incubating the immobilized antigen with the sample to form an immobilized antigen-antibody complex, c) incubating the immobilized antigen-antibody complex with the antigen conjugated to a detectable label to form an immobilized ternary complex, and d) determining the amount of the antibody by determining the amount of the detectable label in the immobilized ternary complex.

公开(公告)号：US20230009655A1

公开(公告)日：2023-01-12

申请号：US17842651

申请日：2022-06-16

申请人： NOUL CO., LTD.

发明人： Dong Young LEE ,  Chan Yang LIM ,  Kyung Hwan KIM

IPC分类号： G01N1/31 ,  G01N1/30 ,  C12Q1/6844 ,  C12Q1/6848 ,  G01N33/50 ,  G01N21/77 ,  G01N33/558 ,  B01L3/00 ,  G01N15/06 ,  G01N33/52 ,  G01N33/574 ,  G01N15/14 ,  G01N33/49 ,  G01N33/60 ,  C12Q1/686 ,  C07K16/30 ,  C12Q1/70 ,  G01N33/483 ,  G06T7/00 ,  G01N33/53 ,  G01N33/533

摘要： The present disclosure relates to an immunoassay method for performing immunoassay by using a patch that contains antibodies. An immunoassay method according to an aspect of the present disclosure performs diagnosis by detecting a target protein from a sample to be diagnosed by using a patch which includes a mesh structural body forming micro-cavities and is configured to contain a liquid substance in the micro-cavities, and includes placing the sample to be diagnosed in a reaction region, and providing an antibodies that react specifically with a target protein to the reaction region by using a patch that contains the antibodies.