公开(公告)号：US12234498B2

公开(公告)日：2025-02-25

申请号：US17819151

申请日：2022-08-11

Applicant: Abbott Diabetes Care Inc.

Inventor： Tianmei Ouyang ,  Benjamin J. Feldman

IPC: G01N27/27 ,  A61B5/1486 ,  C12N11/082 ,  C12Q1/00 ,  C12Q1/26 ,  G01N27/327 ,  G01N33/573 ,  G01N33/66

Abstract: NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

公开(公告)号：US12215374B2

公开(公告)日：2025-02-04

申请号：US17055359

申请日：2019-05-15

Applicant: LIFE SCIENCE BIOSENSOR DIAGNOSTICS PTY LTD

Inventor： Paul Dastoor

IPC: C12Q1/00 ,  C12Q1/26 ,  G01N27/414 ,  H01L51/00 ,  H01L51/05 ,  H10K10/46 ,  H10K71/60 ,  H10K85/10 ,  A61B5/145

Abstract: The present invention relates to organic thin film sensors and the preparation and use thereof in sensing applications, and in particular in glucose sensing. The sensor is characterised by a layered structure comprising a porous wicking layer whose surface is configured to receive a liquid sample. An enzyme is disposed on or within the porous layer for facilitating the generation of a charge carrier from an analyte. A polymer layer in contact with the porous layer is connected to an ohmic conductor for applying a gate voltage to the polymer layer, the polymer layer being conductive to the charge carrier; and an organic semiconducting layer is connected to a source electrode and a drain electrode.

公开(公告)号：US20240294967A1

公开(公告)日：2024-09-05

申请号：US18569532

申请日：2023-01-18

Applicant: Illumina, Inc.

Inventor： Xiaolin WU ,  Antoine FRANCAIS ,  Xiaohai LIU

IPC: C12Q1/6806 ,  C12Q1/26 ,  C12Q1/48 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  C12Q1/26 ,  C12Q1/48 ,  C12Q1/6869

Abstract: Embodiments of the present disclosure relates to various bisulfite-free chemical methods for detecting methylation of cytosine in the DNA sample. These methods convert methylated and hydroxymethylated cytosine in the nucleic acid sequence to a modified or pseudo thymine or a uracil moiety which then can be detected in sequencing.

公开(公告)号：US20240288451A1

公开(公告)日：2024-08-29

申请号：US18562048

申请日：2022-05-18

Applicant: 7905122 CANADA INC.

Inventor： John Manioudakis ,  Kim Beauregard ,  Paul Jollez

IPC: G01N33/72 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/54 ,  G01N33/52

CPC classification number: G01N33/725 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/54 ,  G01N33/528

Abstract: A chromogenic absorbent material for hemoglobin and glucose detection in an animal excretion is provided. The chromogenic absorbent material comprises a benzidine-type compound, an organic hydroperoxide, an oxidoreductase, a peroxidase, a pseudoperoxidase or a combination thereof, and a polysaccharide matrix, comprising: from about 10 wt % to about 32.5 wt % chemically or mechanically processed non-functionalized cellulose; from about 25 wt % to about 70 wt % pregelatinized starch (PGS); from 0 wt % to about 20 wt % guar gum; from 0 wt % to about 25 wt % methyl hydroxyethyl cellulose (MHEC); from 0 wt % to about 15 wt % hydroxyethyl cellulose (HEC); and from 0 wt % to about 10 wt % carboxymethyl cellulose (CMC).

公开(公告)号：US20240271185A1

公开(公告)日：2024-08-15

申请号：US18569192

申请日：2022-08-16

Applicant: Illumina, Inc.

Inventor： Colin Brown ,  Xiaohai Liu ,  Xiaolin Wu ,  Eric Brustad ,  Sarah E. Shultzaberger

IPC: C12Q1/6806 ,  C12Q1/26 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  C12Q1/26 ,  C12Q1/6869

Abstract: Disclosed herein include methods, compositions, reaction mixtures, kits and systems for identification of methylated cytosines in nucleic acids using a bisulfite-free, one-step chemoenzymatic modification of methylated cytosines.

公开(公告)号：US20240254541A1

公开(公告)日：2024-08-01

申请号：US18588930

申请日：2024-02-27

Applicant: The Children's Medical Center Corporation ,  The United States of America, As Represented by the Secretary, Department of Health & Human Services

Inventor： Anjana Rao ,  Mamta Tahiliani ,  Kian Peng Koh ,  Suneet Agarwal ,  Aravind Iyer

IPC: C12Q1/6827 ,  C12N9/10 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/53

CPC classification number: C12Q1/6827 ,  C12N9/1007 ,  C12Q1/26 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N33/5308 ,  C12N2501/70 ,  C12N2501/71 ,  C12N2501/724 ,  C12Q2521/531 ,  C12Q2522/10 ,  C12Q2537/164 ,  C12Q2600/154

Abstract: The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.

公开(公告)号：US20240247297A1

公开(公告)日：2024-07-25

申请号：US18597704

申请日：2024-03-06

Applicant: Abbott Diabetes Care Inc.

Inventor： Tianmei OUYANG ,  Benjamin J. FELDMAN

IPC: C12Q1/00 ,  A61B5/1486 ,  C12N11/082 ,  C12Q1/26 ,  G01N27/27 ,  G01N27/327 ,  G01N33/573 ,  G01N33/66

CPC classification number: C12Q1/001 ,  A61B5/14865 ,  C12N11/082 ,  C12Q1/004 ,  C12Q1/005 ,  C12Q1/006 ,  C12Q1/26 ,  G01N27/3275 ,  G01N33/5735 ,  G01N33/66 ,  C12Y106/05002 ,  G01N27/27

Abstract: NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P)+) or derivative thereof; an NAD(P)+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

公开(公告)号：US20240191212A1

公开(公告)日：2024-06-13

申请号：US18491078

申请日：2023-10-20

Applicant: LANKENAU INSTITUTE FOR MEDICAL RESEARCH

Inventor： George C. Prendergast ,  Richard Metz

IPC: C12N9/02 ,  A01K67/0276 ,  C12Q1/26

CPC classification number: C12N9/0069 ,  A01K67/0276 ,  C12Q1/26 ,  C07K2319/21 ,  C07K2319/40 ,  C12N2830/006

Abstract: The nucleotide and amino acid sequences of indoleamine 2,3-dioxygenase-2 (IDO2) and methods of use thereof are provided.

公开(公告)号：US20240026416A1

公开(公告)日：2024-01-25

申请号：US18320182

申请日：2023-05-18

Applicant: NEOGEN FOOD SAFETY US HOLDCO CORPORATION

Inventor： Jason W. Bjork ,  Adam James Stanenas ,  Wensheng Xia

IPC: C12Q1/04 ,  C12Q1/26 ,  C12N1/20

CPC classification number: C12Q1/04 ,  C12Q1/26 ,  C12N1/20

Abstract: A culture system for culturing a microaerophilic or an anaerobic microorganism is provided. The culture system can include effective amounts of i) an enzyme of an oxidoreductase family and ii) a substrate for said enzyme, a container, and a predetermined volume of aqueous medium that supports growth of said anaerobic or microaerophilic microorganism. The enzyme can be selected from a group consisting of ascorbic acid oxidase and laccase. The effective amounts are effective to deplete dissolved oxygen in the predetermined volume to a concentration that facilitates growth of a microaerophilic microorganism or an obligately-anaerobic microorganism. A method of using the system is also provided.

公开(公告)号：US20230384255A1

公开(公告)日：2023-11-30

申请号：US18034216

申请日：2021-11-22

Applicant: Roche Diabetes Care, Inc.

Inventor： Arnfried Guenther ,  Alexander Steck

IPC: G01N27/327 ,  C12Q1/00 ,  C12Q1/26

CPC classification number: G01N27/3271 ,  C12Q1/006 ,  C12Q1/26 ,  A61B5/1486

Abstract: The present invention relates to a method for the preparation of a working electrode, the method comprising application of a sensing material in several steps. Further, the present invention relates to an analyte sensor comprising the working electrode as well as to the use of the analyte sensor for detecting at least one analyte in a sample.