公开(公告)号：US20030077769A1

公开(公告)日：2003-04-24

申请号：US10149821

申请日：2002-09-18

发明人： Andrew John Smallridge ,  Maurice Arthur Trewhella ,  Kylie Anne Wilkinson

IPC分类号： C12P007/42 ,  C12P007/22 ,  C12N001/18

CPC分类号： C12P7/26 ,  Y02P20/544

摘要： The invention provides a method of synthesis of a substituted or unsubstituted carbinol compound, comprising the steps of subjecting the corresponding substituted or unsubstituted aromatic aldehyde to acyloin condensation mediated by yeast in the presence of either (a) a supercritical fluid or (b) a liquefied gas, and recovering the carbinol compound. Preferably the yeast is Saccharomyces cerevisiae. In a particularly preferred embodiment the aromatic aldehyde is benzaldehyde and the carbinol is pheny-lacetylcarbinol, according to the reaction (1): in which the benzaldehyde, the pyruvic acid, or both may optionally be substituted.

摘要翻译： 本发明提供了一种合成取代或未取代的甲醇化合物的方法，包括以下步骤：在（a）超临界流体或（b）液化的（a）超临界流体存在下，使相应的取代或未取代的芳族醛经酵母介导的偶姻缩合 气体，并回收甲醇化合物。 优选地，酵母是酿酒酵母（Saccharomyces cerevisiae）。 在特别优选的实施方案中，根据反应（1），芳族醛是苯甲醛，甲醇是苯基 - 乙酰基甲醇，其中苯甲醛，丙酮酸或二者可以任选地被取代。

公开(公告)号：US20020039770A1

公开(公告)日：2002-04-04

申请号：US09921260

申请日：2001-08-02

发明人： Michael G. Bramucci ,  Robert Dicosimo ,  Robert Fallon ,  John E. Gavagan ,  Frank Herkes ,  Lech Wilczek

IPC分类号： C12P007/42

CPC分类号： C12P7/625 ,  C08G63/06 ,  C08G63/85 ,  C12P7/42 ,  C12R1/01 ,  C12R1/40

摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for null-caprolactone in the preparation of highly branched copolyester.

摘要翻译： 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 在制备高支化共聚酯时，使用3-羟基戊酸作为ε-己内酯的替代物。

公开(公告)号：US20030134402A1

公开(公告)日：2003-07-17

申请号：US10004115

申请日：2001-12-06

发明人： Hiroyuki Asako ,  Kenji Matsumura ,  Masatoshi Shimizu ,  Nobuya Ito ,  Ryuhei Wakita

IPC分类号： C12P007/42 ,  C12N009/02 ,  C12N009/18 ,  C07H021/04

CPC分类号： C12N9/0006 ,  C12P7/42 ,  C12P7/62 ,  C12P13/002

摘要： There are provided a polynucleotide sequence coding for an amino acid sequence capable of preferentially producing (S)-4-bromo-3-hydroxy-butanoate by asymmetrically reducing 4-bromo-3-oxobutanoate, A DNA construct having a promoter in operative linkage with the polynucleotide sequence, a recombinant vector containing the polynucleotide sequences a transformant, a recombinant vector and the like.

摘要翻译： 提供了编码能够通过不对称还原4-溴-3-氧代丁酸酯优先产生（S）-4-溴-3-羟基丁酸酯的氨基酸序列的多核苷酸序列，具有与 多核苷酸序列，含有多核苷酸序列的重组载体，转化体，重组载体等。

公开(公告)号：US20010053847A1

公开(公告)日：2001-12-20

申请号：US09765873

申请日：2001-01-19

发明人： Xiao-Song Tang

IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12P005/02 ,  C12P007/42 ,  C12N001/20

CPC分类号： C12N9/0042 ,  C12N9/0077 ,  C12N9/88 ,  C12N15/8243 ,  C12P7/42

摘要： The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

摘要翻译： 本发明提供了几种生物制备对羟基肉桂酸（PHCA）的方法。 本发明还涉及具有将肉桂酸转化为PHCA的能力的新真菌和细菌的发现。 本发明涉及通过将来自酵母红酵母的野生型PAL掺入到大肠杆菌中的葡萄糖转化为PHCA的新的生物催化剂的开发，其强调野生型PAL将酪氨酸转化为PHCA的能力。 本发明还涉及开发新的生物催化剂，用于通过将来自酵母红酵母（Rhodotorula glutinis）加植物细胞色素P-450和细胞色素P-450还原酶的野生型PAL掺入大肠杆菌将葡萄糖转化为PHCA。 在另一个实施方案中，本发明提供了通过具有增强的酪氨酸氨裂解酶（TAL）活性的野生型酵母PAL的诱变来开发新的生物催化剂。

公开(公告)号：US20010041359A1

公开(公告)日：2001-11-15

申请号：US09834926

申请日：2001-04-16

发明人： Peter Pochlauer ,  Herbert Mayrhofer

IPC分类号： C12P007/42 ,  C07C051/42

CPC分类号： C07C51/08 ,  C07C51/43 ,  C07C59/56

摘要： Process for the preparation of optically highly pure (R)null and (S)nullnull-hydroxycarboxylic acids, in which either isolated, impure (R)null and (S)nullnull-hydroxycarboxylic acids prepared by acidic hydrolysis of the (R)null and (S)nullcyanohydrins obtained by enzyme-catalyzed addition of a cyanide group donor to the corresponding aldehydes or ketones are recrystallized in an aromatic hydrocarbon, optionally in the presence of a cosolvent, and optically highly pure (R)null and (S)nullnull-hydroxycarboxylic acids having an optical purity of over 98%ee are obtained or the hydrolysis solution obtained by acidic hydrolysis of the (R)null and (S)nullcyanohydrins is treated directly with an aromatic hydrocarbon, optionally in combination with a cosolvent, and is then extracted at hydrolysis temperature, whereupon after cooling of the organic phase the corresponding chemically and optically highly pure (R)null and (S)nullnull-hydroxycarboxylic acids having an optical purity of over 98%ee crystallize out.

摘要翻译： 用于制备光学高纯度（R） - 和（S）-α-羟基羧酸的方法，其中通过（R） - （S）-α-羟基羧酸的酸性水解制备的分离的，不纯的（R） - 和（S） - 和（S） - 氰基醇通过酶催化加成氰化物基团给予相应的醛或酮在芳香烃中任选在共溶剂存在下重结晶，和光学上高纯度（R） - 和（S ） - 光学纯度超过98％ee的α-羟基羧酸，或者通过（R） - 和（S） - 氰基醇的酸性水解获得的水解溶液直接用芳烃处理，任选地与 共溶剂，然后在水解温度下提取，随后有机相冷却后，光学纯度超过98％ee的相应的化学和光学高纯度（R） - 和（S）-α-羟基羧酸结晶出来。

公开(公告)号：US20040002147A1

公开(公告)日：2004-01-01

申请号：US10241742

申请日：2002-09-09

发明人： Grace Desantis ,  Jennifer Ann Chaplin ,  Ellen Chi ,  Aileen Milan ,  Jay M. Short ,  David Weiner ,  Mark J. Burk ,  Eric J. Mathur ,  Darcy Madden (Heir)

IPC分类号： C12P013/04 ,  C12P007/42 ,  C12N009/78 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

CPC分类号： C12P7/42 ,  C12N9/78 ,  C12P13/004 ,  C12P13/04 ,  C12P41/006

摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

摘要翻译： 本发明涉及腈水解酶和编码腈水解酶的核酸。 另外还提​​供了设计新腈水解酶的方法及其使用方法。 在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

公开(公告)号：US20030186400A1

公开(公告)日：2003-10-02

申请号：US10327108

申请日：2002-12-24

申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

发明人： Hiroyuki Asako ,  Ryuhei Wakita ,  Nobuya Itoh

IPC分类号： C12P007/62 ,  C12P007/42 ,  C12N009/02 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

CPC分类号： C12N9/0008 ,  C12P7/42 ,  C12P7/62

摘要： There is disclosed a method for producing optically active 2-hydroxycycloalkanecarboxylic acid characterized by the steps of: allowing 2-oxocycloalkanecarboxylic acid ester to react with a transformant or a dead cell artificially provided with an ability to asymmetrically reduce 2-oxocycloalkanecarboxylic acid ester to optically active 2-hydroxycycloalkanecarboxylic acid ester and an ability to regenerate a coenzyme on which an enzyme having the former ability depends; and collecting the produced optically active 2-hydroxycycloalkanecarboxylic acid ester.

摘要翻译： 公开了光学活性2-羟基环烷烃羧酸的制造方法，其特征在于，使2-氧代环烷烃羧酸酯与转化体或死细胞反应，所述转化体或死细胞具有将2-氧代环烷烃羧酸酯不对称地还原成光学活性的能力 2-羟基环烷羧酸酯和再生具有先前能力的酶所依赖的酶的辅酶的能力; 并收集所产生的光学活性2-羟基环烷羧酸酯。

公开(公告)号：US20020142407A1

公开(公告)日：2002-10-03

申请号：US10042991

申请日：2002-01-09

申请人： Firmenich SA

发明人： Ian Michael Whitehead ,  Alan John Slusarenko ,  Urs Waspi ,  Duncan James Horatio Gaskin ,  Alan Richard Brash ,  Nathalie Tijet

IPC分类号： C12P007/40 ,  C12P007/42 ,  C12P007/02

CPC分类号： C12P7/42 ,  C12N9/88 ,  C12P7/02 ,  C12P7/40

摘要： The present invention relates to fatty acid 13-hydroperoxide lyase protein from guava (Psidium guajava) and the gene encoding the protein. Expression systems for recombinant guava 13-hydroperoxide lyase and methods of using recombinant guava 13-hydroperoxide lyase for the production of green notes are provided.

摘要翻译： 本发明涉及来自番石榴（Psidium guajava）的脂肪酸13-氢过氧化物裂解酶蛋白质和编码该蛋白质的基因。 提供重组番石榴13-氢过氧化物裂解酶的表达系统和使用重组番石榴13-氢过氧化物裂解酶生产绿色笔记的方法。

公开(公告)号：US20040053382A1

公开(公告)日：2004-03-18

申请号：US10433835

申请日：2003-06-09

发明人： Richard F. Senkpeil ,  David P. Pantaleone ,  Paul P. Taylor

IPC分类号： C12P007/42

CPC分类号： C12P7/42 ,  C12N15/52 ,  Y02P20/582

摘要： An economical and expedient method is disclosed for the preparation of null-hydroxy-carboxylic acids or salts thereof in very high enantiomeric purity which comprises oxidizing a corresponding null-amino-carboxylic acid or salt thereof using an amino acid deaminase followed by reducing the corresponding null-keto-carboxylic acid or salt produced using a D- or L-lactate dehydrogenase in the combination with an electron donor and an enzyme/substrate system for recycling the electron donor. The resulting null-hydroxy-carboxylic acids, hydrates, and salts thereof are valuable components and intermediates in the preparation of chiral compounds, especially pharmaceuticals. This invention also relates to the use of null-amino-carboxylic acids, hydrates, and salts thereof and a coupled enzyme system in the production of null-hydroxy-carboxylic acids, hydrates, and salts thereof.

摘要翻译： 公开了一种用于制备非常高对映体纯度的α-羟基 - 羧酸或其盐的经济和方便的方法，其包括使用氨基酸脱氨酶氧化相应的α-氨基 - 羧酸或其盐，随后还原相应的α - 酮 - 羧酸或使用D-或L-乳酸脱氢酶与电子给体和用于再循环电子给体的酶/底物系统组合产生的盐。 所得的α-羟基羧酸，水合物和盐是制备手性化合物，特别是药物的有价值的成分和中间体。 本发明还涉及α-氨基羧酸，水合物及其盐的用途以及在制备α-羟基 - 羧酸，水合物及其盐中的偶联酶系统。

公开(公告)号：US20040005678A1

公开(公告)日：2004-01-08

申请号：US10411066

申请日：2003-04-09

发明人： Jay Keasling ,  Vincent Martin ,  Douglas Pitera ,  Sydnor T. Withers III ,  Jack Newman

IPC分类号： C12P007/42 ,  C12N009/10 ,  C07H021/04 ,  C12N001/21 ,  C12N015/74

CPC分类号： C12N9/88 ,  C12N9/1205 ,  C12N15/52 ,  C12P5/007

摘要： Methods for synthesizing amorpha-4,11-diene synthase from isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. Amorpha-4,11-diene synthase is then produced using an optimized amorpha-4,11-diene synthase gene. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.

摘要翻译： 提供了从异戊烯焦磷酸酯合成紫罗兰-4,11-二烯合成酶的方法。 第一种方法包括向宿主微生物中引入多个异源核酸序列，每个异源核酸序列编码甲羟戊酸途径中用于产生异戊烯基焦磷酸盐的不同酶。 然后使用优化的Amorpha-4,11-二烯合酶基因产生Amorpha-4,11-二烯合酶。 本发明还提供了用于实施该方法的核酸序列，酶，表达载体和转化的宿主细胞。