公开(公告)号：US20040171119A1

公开(公告)日：2004-09-02

申请号：US10782998

申请日：2004-02-20

Applicant: Sumitomo Chemical Company, Limited

Inventor： Nobuya Itoh

IPC: C12N009/02 ,  C07H021/04

CPC classification number: C12N9/0006 ,  C12P7/22 ,  C12R1/01

Abstract: A gene containing a DNA having the nucleotide sequence encoding any one of the amino acid sequences of the following (a) to (e): (a) an amino acid sequence set out in SEQ ID NO: 1; (b) an amino acid sequence having the sequence homology of 80% or more with (a); (c) an amino acid sequence having the sequence homology of 90% or more with (a); (d) an amino acid sequence encoded by a DNA having the nucleotide sequence set out in SEQ ID NO: 2; (e) an amino acid sequence encoded by a DNA having the nucleotide sequence having the sequence homology of 80% or more with (d).

Abstract translation: 含有具有编码以下（a）〜（e）的氨基酸序列的任一个的核苷酸序列的DNA的基因：（a）SEQ ID NO：1所示的氨基酸序列; （b）与（a）的序列同源性为80％以上的氨基酸序列; （c）与（a）的序列同源性为90％以上的氨基酸序列; （d）由具有SEQ ID NO：2所示核苷酸序列的DNA编码的氨基酸序列; （e）由具有（d）的序列同源性为80％以上的核苷酸序列的DNA编码的氨基酸序列。

公开(公告)号：US20030186400A1

公开(公告)日：2003-10-02

申请号：US10327108

申请日：2002-12-24

Applicant: SUMITOMO CHEMICAL COMPANY, LIMITED

Inventor： Hiroyuki Asako ,  Ryuhei Wakita ,  Nobuya Itoh

IPC: C12P007/62 ,  C12P007/42 ,  C12N009/02 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

CPC classification number: C12N9/0008 ,  C12P7/42 ,  C12P7/62

Abstract: There is disclosed a method for producing optically active 2-hydroxycycloalkanecarboxylic acid characterized by the steps of: allowing 2-oxocycloalkanecarboxylic acid ester to react with a transformant or a dead cell artificially provided with an ability to asymmetrically reduce 2-oxocycloalkanecarboxylic acid ester to optically active 2-hydroxycycloalkanecarboxylic acid ester and an ability to regenerate a coenzyme on which an enzyme having the former ability depends; and collecting the produced optically active 2-hydroxycycloalkanecarboxylic acid ester.

Abstract translation: 公开了光学活性2-羟基环烷烃羧酸的制造方法，其特征在于，使2-氧代环烷烃羧酸酯与转化体或死细胞反应，所述转化体或死细胞具有将2-氧代环烷烃羧酸酯不对称地还原成光学活性的能力 2-羟基环烷羧酸酯和再生具有先前能力的酶所依赖的酶的辅酶的能力; 并收集所产生的光学活性2-羟基环烷羧酸酯。